Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis

11 páginas, 7 figuras. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/133/21/4257/DC1

Detalles Bibliográficos
Autores: Simões, Sergio, Denholm, Barry, Azevedo, Dulce, Sotillos, Sol, Martín, Paul, Skaer, Helen, Castelli-Gair Hombría, James, Jacinto, Antonio
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/41261
Acceso en línea:http://hdl.handle.net/10261/41261
Access Level:acceso abierto
Palabra clave:Cell invagination
Myosin II
Actin cytoskeleton
Small GTPase Rho1
RhoGEFs
RhoGAPs
Posterior spiracles
Drosophila
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spelling Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesisSimões, SergioDenholm, BarryAzevedo, DulceSotillos, SolMartín, PaulSkaer, HelenCastelli-Gair Hombría, JamesJacinto, AntonioCell invaginationMyosin IIActin cytoskeletonSmall GTPase Rho1RhoGEFsRhoGAPsPosterior spiraclesDrosophila11 páginas, 7 figuras. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/133/21/4257/DC1During development, small RhoGTPases control the precise cell shape changes and movements that underlie morphogenesis. Their activity must be tightly regulated in time and space, but little is known about how Rho regulators (RhoGEFs and RhoGAPs) perform this function in the embryo. Taking advantage of a new probe that allows the visualisation of small RhoGTPase activity in Drosophila, we present evidence that Rho1 is apically activated and essential for epithelial cell invagination, a common morphogenetic movement during embryogenesis. In the posterior spiracles of the fly embryo, this asymmetric activation is achieved by at least two mechanisms: the apical enrichment of Rho1; and the opposing distribution of Rho activators and inhibitors to distinct compartments of the cell membrane. At least two Rho1 activators, RhoGEF2 and RhoGEF64C are localised apically, whereas the Rho inhibitor RhoGAP Cv-c localises at the basolateral membrane. Furthermore, the mRNA of RhoGEF64C is also apically enriched, depending on signals present within its open reading frame, suggesting that apical transport of RhoGEF mRNA followed by local translation is a mechanism to spatially restrict Rho1 activity during epithelial cell invagination.This work was supported by grants from Fundação para a Ciência e Tecnologia, Portugal and European Union Framework Programme 6 (Network of Excellence Cells into Organs) to A.J. and by a grant from the Ministerio de Educación y Ciencia, Spain/FEDER BFU2004-1069 to J.H.Peer reviewedCompany of Biologists201120112006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/41261reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/dev.02588info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/412612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
title Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
spellingShingle Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
Simões, Sergio
Cell invagination
Myosin II
Actin cytoskeleton
Small GTPase Rho1
RhoGEFs
RhoGAPs
Posterior spiracles
Drosophila
title_short Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
title_full Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
title_fullStr Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
title_full_unstemmed Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
title_sort Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis
dc.creator.none.fl_str_mv Simões, Sergio
Denholm, Barry
Azevedo, Dulce
Sotillos, Sol
Martín, Paul
Skaer, Helen
Castelli-Gair Hombría, James
Jacinto, Antonio
author Simões, Sergio
author_facet Simões, Sergio
Denholm, Barry
Azevedo, Dulce
Sotillos, Sol
Martín, Paul
Skaer, Helen
Castelli-Gair Hombría, James
Jacinto, Antonio
author_role author
author2 Denholm, Barry
Azevedo, Dulce
Sotillos, Sol
Martín, Paul
Skaer, Helen
Castelli-Gair Hombría, James
Jacinto, Antonio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell invagination
Myosin II
Actin cytoskeleton
Small GTPase Rho1
RhoGEFs
RhoGAPs
Posterior spiracles
Drosophila
topic Cell invagination
Myosin II
Actin cytoskeleton
Small GTPase Rho1
RhoGEFs
RhoGAPs
Posterior spiracles
Drosophila
description 11 páginas, 7 figuras. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/133/21/4257/DC1
publishDate 2006
dc.date.none.fl_str_mv 2006
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/41261
url http://hdl.handle.net/10261/41261
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/dev.02588
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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