Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

Reactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine inve...

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Detalhes bibliográficos
Autores: Diaz de Cerio Arruabarrena, Oihane, Reina, Lander, Squatrito, Valeria, Etxebarria Loizate, Nestor, González Gaya, Belén, Cancio Uriarte, Ibon
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42590
Acesso em linha:http://hdl.handle.net/10810/42590
Access Level:acceso abierto
Palavra-chave:Mytilus galloprovincialis ovothiol
ovothiol synthase
glutathione
oxidative stress
metabolome
oocytes
pollution biomonitoring
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spelling Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted WatersDiaz de Cerio Arruabarrena, OihaneReina, LanderSquatrito, ValeriaEtxebarria Loizate, NestorGonzález Gaya, BelénCancio Uriarte, IbonMytilus galloprovincialis ovothiolovothiol synthaseglutathioneoxidative stressmetabolomeoocytespollution biomonitoringReactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization.Basque Government through project IT1302-19 and Spanish MINECO through project EU-FEDER/ERDF AGL2015-63936-R. B.G.G. is the recipient of a postdoctoral Juan de la Cierva fellowship of the Spanish Ministry of Science and Innovation.MDPI2020202020202020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/42590reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL2015-63936-R/https://www.mdpi.com/2218-273X/10/3/373info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)oai:addi.ehu.eus:10810/425902026-06-18T09:23:17Z
dc.title.none.fl_str_mv Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
title Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
spellingShingle Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
Diaz de Cerio Arruabarrena, Oihane
Mytilus galloprovincialis ovothiol
ovothiol synthase
glutathione
oxidative stress
metabolome
oocytes
pollution biomonitoring
title_short Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
title_full Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
title_fullStr Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
title_full_unstemmed Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
title_sort Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters
dc.creator.none.fl_str_mv Diaz de Cerio Arruabarrena, Oihane
Reina, Lander
Squatrito, Valeria
Etxebarria Loizate, Nestor
González Gaya, Belén
Cancio Uriarte, Ibon
author Diaz de Cerio Arruabarrena, Oihane
author_facet Diaz de Cerio Arruabarrena, Oihane
Reina, Lander
Squatrito, Valeria
Etxebarria Loizate, Nestor
González Gaya, Belén
Cancio Uriarte, Ibon
author_role author
author2 Reina, Lander
Squatrito, Valeria
Etxebarria Loizate, Nestor
González Gaya, Belén
Cancio Uriarte, Ibon
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mytilus galloprovincialis ovothiol
ovothiol synthase
glutathione
oxidative stress
metabolome
oocytes
pollution biomonitoring
topic Mytilus galloprovincialis ovothiol
ovothiol synthase
glutathione
oxidative stress
metabolome
oocytes
pollution biomonitoring
description Reactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/42590
url http://hdl.handle.net/10810/42590
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL2015-63936-R/
https://www.mdpi.com/2218-273X/10/3/373
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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