Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius

28 pages, 4 figures.--Article first published online: 27 FEB 2015

Detalles Bibliográficos
Autores: Crespo Sempere, Ana, Selma Lázaro, Cristina, Palumbo, Jeffrey D., González-Candelas, Luis, Martínez-Culebras, Pedro V.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127715
Acceso en línea:http://hdl.handle.net/10261/127715
Access Level:acceso abierto
Palabra clave:Aspergillus carbonarius
Ochratoxin production
Oxidative stress
Grapes
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spelling Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonariusCrespo Sempere, AnaSelma Lázaro, CristinaPalumbo, Jeffrey D.González-Candelas, LuisMartínez-Culebras, Pedro V.Aspergillus carbonariusOchratoxin productionOxidative stressGrapes28 pages, 4 figures.--Article first published online: 27 FEB 2015BACKGROUND:There are few studies dealing with the relationship between oxidative stress and ochratoxin A (OTA) biosynthesis. In this work, we analyzed the effect of the oxidant stressor menadione and the antioxidants 3,5-di-tert-butyl-4-hydroxytoluene (BHT), catechin, resveratrol and a polyphenolic extract on growth, generation of reactive oxygen species (ROS), OTA production and gene expression of antioxidant enzymes of Aspergillus carbonarius. RESULTS:Exposure to menadione concentrations higher than 20 µmol L−1 led to increases in ROS and OTA levels and a decrease in growth rate. Exposure to 2.5–10 mmol L−1 BHT also led to higher ROS and OTA levels, although growth rate was only affected above 5 mmol L−1. Naturally occurring concentrations of catechin, resveratrol and polyphenolic extract barely affected growth rate, but they produced widely different effects on OTA production level depending on the antioxidant concentration used. In general, gene expression of antioxidant enzymes superoxide dismutase (SOD) and peroxiredoxin (PRX) was downregulated after exposure to oxidant and antioxidant concentrations that enhanced OTA production level. CONCLUSION:Aspergillus carbonarius responds to oxidative stress, increasing OTA production. Nevertheless, the use of naturally occurring concentrations of antioxidant phenolic compounds to reduce oxidative stress is not a valid approach by itself for OTA contamination control in grapes.A Crespo-Sempere is the recipient of an FPI fellowship from the Spanish Government. This research was supported by grants AGL2005-00707 and AGL2008-04828-C03-02 from the Spanish Government.Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127715reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/jsfa.7077Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1277152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
title Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
spellingShingle Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
Crespo Sempere, Ana
Aspergillus carbonarius
Ochratoxin production
Oxidative stress
Grapes
title_short Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
title_full Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
title_fullStr Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
title_full_unstemmed Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
title_sort Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
dc.creator.none.fl_str_mv Crespo Sempere, Ana
Selma Lázaro, Cristina
Palumbo, Jeffrey D.
González-Candelas, Luis
Martínez-Culebras, Pedro V.
author Crespo Sempere, Ana
author_facet Crespo Sempere, Ana
Selma Lázaro, Cristina
Palumbo, Jeffrey D.
González-Candelas, Luis
Martínez-Culebras, Pedro V.
author_role author
author2 Selma Lázaro, Cristina
Palumbo, Jeffrey D.
González-Candelas, Luis
Martínez-Culebras, Pedro V.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Aspergillus carbonarius
Ochratoxin production
Oxidative stress
Grapes
topic Aspergillus carbonarius
Ochratoxin production
Oxidative stress
Grapes
description 28 pages, 4 figures.--Article first published online: 27 FEB 2015
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127715
url http://hdl.handle.net/10261/127715
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/jsfa.7077
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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