3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation

The control of cell-microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with a precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, the...

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Detalhes bibliográficos
Autores: Xiao, Miao, Ulloa Severino, Francesco Paolo, Iseppon, Federico, Cheng, Guosheng, Torre, Vincent, Tang, Mingliang
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414135
Acesso em linha:http://hdl.handle.net/10261/414135
https://api.elsevier.com/content/abstract/scopus_id/85092945106
Access Level:Acceso aberto
Palavra-chave:Three dimension
electrical activity
geometric regulation
neuronal network
ordered graphene
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network FormationXiao, MiaoUlloa Severino, Francesco PaoloIseppon, FedericoCheng, GuoshengTorre, VincentTang, MingliangThree dimensionelectrical activitygeometric regulationneuronal networkordered graphenehttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesThe control of cell-microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with a precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, they form well-defined 3D connections. Astrocytes have a more ramified shape similar to that seen in vivo because of the nanosized ripples and wrinkles on the surface of graphene skeleton. Neurons have axons and dendrites aligned along the graphene skeleton, allowing the formation of neuronal networks with highly controlled connections. Neuronal networks have higher electrical activity with functional signaling over a long distance along the graphene skeleton. Our study, for the first time, investigated the geometrical cues on ordered neuronal growth and network formation with the support of graphene in 3D, which therefore advanced the development of customized scaffolds for brain-machine interfaces or neuroprosthetic devices.We are thankful for the funding provided by the National Natural Science Foundation of China (81970883, 91839101); Natural Science Foundation of Jiangsu Province (SBK2020042688); Regione friuli−venezia giulia (FVG) (Italy) for the project “GLIOBLASTOMA-Infiltrazione nei gliomi: nuovo target terapeutico”; Key Research and Development Program of Jiangsu Province (BE2017665).Peer reviewedACS PublicationsNational Natural Science Foundation of ChinaNatural Science Foundation of Jiangsu ProvinceRegione Autonoma Friuli Venezia GiuliaJiangsu Province0000-0002-4072-81060000-0003-3725-97130000-0003-4810-9403202620262020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/414135https://api.elsevier.com/content/abstract/scopus_id/85092945106reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésNano lettershttps://doi.org/10.1021/acs.nanolett.0c02107Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4141352026-05-22T06:33:51Z
dc.title.none.fl_str_mv 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
title 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
spellingShingle 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
Xiao, Miao
Three dimension
electrical activity
geometric regulation
neuronal network
ordered graphene
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
title_full 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
title_fullStr 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
title_full_unstemmed 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
title_sort 3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation
dc.creator.none.fl_str_mv Xiao, Miao
Ulloa Severino, Francesco Paolo
Iseppon, Federico
Cheng, Guosheng
Torre, Vincent
Tang, Mingliang
author Xiao, Miao
author_facet Xiao, Miao
Ulloa Severino, Francesco Paolo
Iseppon, Federico
Cheng, Guosheng
Torre, Vincent
Tang, Mingliang
author_role author
author2 Ulloa Severino, Francesco Paolo
Iseppon, Federico
Cheng, Guosheng
Torre, Vincent
Tang, Mingliang
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Natural Science Foundation of Jiangsu Province
Regione Autonoma Friuli Venezia Giulia
Jiangsu Province
0000-0002-4072-8106
0000-0003-3725-9713
0000-0003-4810-9403
dc.subject.none.fl_str_mv Three dimension
electrical activity
geometric regulation
neuronal network
ordered graphene
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic Three dimension
electrical activity
geometric regulation
neuronal network
ordered graphene
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description The control of cell-microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with a precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, they form well-defined 3D connections. Astrocytes have a more ramified shape similar to that seen in vivo because of the nanosized ripples and wrinkles on the surface of graphene skeleton. Neurons have axons and dendrites aligned along the graphene skeleton, allowing the formation of neuronal networks with highly controlled connections. Neuronal networks have higher electrical activity with functional signaling over a long distance along the graphene skeleton. Our study, for the first time, investigated the geometrical cues on ordered neuronal growth and network formation with the support of graphene in 3D, which therefore advanced the development of customized scaffolds for brain-machine interfaces or neuroprosthetic devices.
publishDate 2020
dc.date.none.fl_str_mv 2020
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/414135
https://api.elsevier.com/content/abstract/scopus_id/85092945106
url http://hdl.handle.net/10261/414135
https://api.elsevier.com/content/abstract/scopus_id/85092945106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nano letters
https://doi.org/10.1021/acs.nanolett.0c02107
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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