Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization

This article belongs to the Special Issue Antioxidants from Food and Food Wastes for Nutraceutical, Pharmaceutical and Cosmetic Fields.

Detalles Bibliográficos
Autores: Rivera Tovar, Pamela Raquel, Contreras Contreras, Gabriela, Rivas Reyes, Paulina Isabel, Pérez-Jiménez, Jara, Martínez Cifuentes, Maximiliano, Pérez-Correa, José Ricardo, Mariotti-Celis, María Salomé
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/382516
Acceso en línea:http://hdl.handle.net/10261/382516
Access Level:acceso abierto
Palabra clave:Brown seaweed
Integrated extraction–purification processes
Spectrophotometric
UHPLC-QToF MS/MS
Mass balance
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spelling Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterizationRivera Tovar, Pamela RaquelContreras Contreras, GabrielaRivas Reyes, Paulina IsabelPérez-Jiménez, JaraMartínez Cifuentes, MaximilianoPérez-Correa, José RicardoMariotti-Celis, María SaloméBrown seaweedIntegrated extraction–purification processesSpectrophotometricUHPLC-QToF MS/MSMass balanceThis article belongs to the Special Issue Antioxidants from Food and Food Wastes for Nutraceutical, Pharmaceutical and Cosmetic Fields.The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.Financial support was received from FONDECYT Regular projects number 1220097 and 1230115, and FOVI project number 240263.Peer reviewedMultidisciplinary Digital Publishing InstituteFondo Nacional de Desarrollo Científico y Tecnológico (Chile)Agencia Nacional de Investigación y Desarrollo (Chile)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/382516reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésRivera Tovar, Pamela Raquel; Contreras Contreras, Gabriela; Rivas Reyes, Paulina Isabel; Pérez-Jiménez, Jara; Martínez Cifuentes, Maximiliano; Pérez-Correa, José Ricardo; Mariotti-Celis, María Salomé; 2025; Supplementary Materials: Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization [Dataset]; Multidisciplinary Digital Publishing Institute; https://doi.org/10.3390/antiox14030250https://doi.org/10.3390/antiox14030250Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3825162026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
title Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
spellingShingle Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
Rivera Tovar, Pamela Raquel
Brown seaweed
Integrated extraction–purification processes
Spectrophotometric
UHPLC-QToF MS/MS
Mass balance
title_short Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
title_full Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
title_fullStr Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
title_full_unstemmed Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
title_sort Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization
dc.creator.none.fl_str_mv Rivera Tovar, Pamela Raquel
Contreras Contreras, Gabriela
Rivas Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author Rivera Tovar, Pamela Raquel
author_facet Rivera Tovar, Pamela Raquel
Contreras Contreras, Gabriela
Rivas Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author_role author
author2 Contreras Contreras, Gabriela
Rivas Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Agencia Nacional de Investigación y Desarrollo (Chile)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brown seaweed
Integrated extraction–purification processes
Spectrophotometric
UHPLC-QToF MS/MS
Mass balance
topic Brown seaweed
Integrated extraction–purification processes
Spectrophotometric
UHPLC-QToF MS/MS
Mass balance
description This article belongs to the Special Issue Antioxidants from Food and Food Wastes for Nutraceutical, Pharmaceutical and Cosmetic Fields.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/382516
url http://hdl.handle.net/10261/382516
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Rivera Tovar, Pamela Raquel; Contreras Contreras, Gabriela; Rivas Reyes, Paulina Isabel; Pérez-Jiménez, Jara; Martínez Cifuentes, Maximiliano; Pérez-Correa, José Ricardo; Mariotti-Celis, María Salomé; 2025; Supplementary Materials: Sustainable recovery of phlorotannins from Durvillaea incurvata: Integrated extraction and purification with advanced characterization [Dataset]; Multidisciplinary Digital Publishing Institute; https://doi.org/10.3390/antiox14030250
https://doi.org/10.3390/antiox14030250
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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