Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets

INTRODUCTION: The melanocortin system plays an important role in energy homeostasis. Mice genetically deficient in the melanocortin-3 receptor gene have a normal body weight with increased body fat, mild hypophagia compared to wild-type mice. In humans, Thr6Lys and Val81Ile variants of the melanocor...

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Autores: Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638, Cruz, R. (R.) de la|||/items/0bf030f8-bd1d-45f1-9a7b-6015e79e71b5, Holst, C. (C.)|||/items/d9318459-9342-49cf-a2da-d6aeb6914a12, Grau, K. (K.)|||/items/dee32024-fa7a-4ab1-8273-3a025e0e73fc, Naranjo, C. (C.)|||/items/9b345f66-f586-434d-84a8-02cfa612745e, Maiz, A. (Alberto)|||/items/ceb4045f-526f-4f1b-9aa1-9ac32e034478, Astrup, A. (Arne)|||/items/f6a0e5d3-b236-40b9-a5a3-c380ae3446f2, Saris, W.H.M. (Wim H. M.)|||/items/72a768f2-c225-4139-86ea-e7895ef2a736, MacDonald, I. (Ian)|||/items/c1052005-3adc-4628-8276-f2803549627f, Oppert, J.M. (Jean M.)|||/items/93cbce83-69c1-4820-9e4b-a4003c9c5629, Hansen, T. (Torben)|||/items/de8447d9-7fad-4ca8-9a14-fbe1493a5e91, Pedersen, O. (Oluf)|||/items/7f57f180-6e9b-44fd-914e-e2f48b9c94e7, Sørensen, T.I.A (Thorkild I. A.)|||/items/be862a34-8bb8-4755-ac24-3ed3689e43d0, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/28170
Acceso en línea:https://hdl.handle.net/10171/28170
Access Level:acceso abierto
Palabra clave:Genome wide association
Childhood obesity
Eating behavior
Food intake
Body fat
Polymorphisms
Insulin
Mutations
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spelling Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat dietsSantos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638Cruz, R. (R.) de la|||/items/0bf030f8-bd1d-45f1-9a7b-6015e79e71b5Holst, C. (C.)|||/items/d9318459-9342-49cf-a2da-d6aeb6914a12Grau, K. (K.)|||/items/dee32024-fa7a-4ab1-8273-3a025e0e73fcNaranjo, C. (C.)|||/items/9b345f66-f586-434d-84a8-02cfa612745eMaiz, A. (Alberto)|||/items/ceb4045f-526f-4f1b-9aa1-9ac32e034478Astrup, A. (Arne)|||/items/f6a0e5d3-b236-40b9-a5a3-c380ae3446f2Saris, W.H.M. (Wim H. M.)|||/items/72a768f2-c225-4139-86ea-e7895ef2a736MacDonald, I. (Ian)|||/items/c1052005-3adc-4628-8276-f2803549627fOppert, J.M. (Jean M.)|||/items/93cbce83-69c1-4820-9e4b-a4003c9c5629Hansen, T. (Torben)|||/items/de8447d9-7fad-4ca8-9a14-fbe1493a5e91Pedersen, O. (Oluf)|||/items/7f57f180-6e9b-44fd-914e-e2f48b9c94e7Sørensen, T.I.A (Thorkild I. A.)|||/items/be862a34-8bb8-4755-ac24-3ed3689e43d0Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Genome wide associationChildhood obesityEating behaviorFood intakeBody fatPolymorphismsInsulinMutationsINTRODUCTION: The melanocortin system plays an important role in energy homeostasis. Mice genetically deficient in the melanocortin-3 receptor gene have a normal body weight with increased body fat, mild hypophagia compared to wild-type mice. In humans, Thr6Lys and Val81Ile variants of the melanocortin-3 receptor gene (MC3R) have been associated with childhood obesity, higher BMI Z-score and elevated body fat percentage compared to non-carriers. The aim of this study is to assess the association in adults between allelic variants of MC3R with weight loss induced by energy-restricted diets. SUBJECTS AND METHODS: This research is based on the NUGENOB study, a trial conducted to assess weight loss during a 10-week dietary intervention involving two different hypo-energetic (high-fat and low-fat) diets. A total of 760 obese patients were genotyped for 10 single nucleotide polymorphisms covering the single exon of MC3R gene and its flanking regions, including the missense variants Thr6Lys and Val81Ile. Linear mixed models and haplotype-based analysis were carried out to assess the potential association between genetic polymorphisms and differential weight loss, fat mass loss, waist change and resting energy expenditure changes. RESULTS: No differences in drop-out rate were found by MC3R genotypes. The rs6014646 polymorphism was significantly associated with weight loss using co-dominant (p = 0.04) and dominant models (p = 0.03). These p-values were not statistically significant after strict control for multiple testing. Haplotype-based multivariate analysis using permutations showed that rs3827103-rs1543873 (p = 0.06), rs6014646-rs6024730 (p = 0.05) and rs3746619-rs3827103 (p = 0.10) displayed near-statistical significant results in relation to weight loss. No other significant associations or gene*diet interactions were detected for weight loss, fat mass loss, waist change and resting energy expenditure changes. CONCLUSION: The study provided overall sufficient evidence to support that there is no major effect of genetic variants of MC3R and differential weight loss after a 10-week dietary intervention with hypo-energetic diets in obese Europeans.Public Library of SciencesDadun. Depósito Académico Digital Universidad de Navarra20132013-03-2220112011-01-0120112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/28170reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/281702026-06-21T12:47:57Z
dc.title.none.fl_str_mv Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
title Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
spellingShingle Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638
Genome wide association
Childhood obesity
Eating behavior
Food intake
Body fat
Polymorphisms
Insulin
Mutations
title_short Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
title_full Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
title_fullStr Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
title_full_unstemmed Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
title_sort Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomised trial of hypo-energetic high- versus low-fat diets
dc.creator.none.fl_str_mv Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638
Cruz, R. (R.) de la|||/items/0bf030f8-bd1d-45f1-9a7b-6015e79e71b5
Holst, C. (C.)|||/items/d9318459-9342-49cf-a2da-d6aeb6914a12
Grau, K. (K.)|||/items/dee32024-fa7a-4ab1-8273-3a025e0e73fc
Naranjo, C. (C.)|||/items/9b345f66-f586-434d-84a8-02cfa612745e
Maiz, A. (Alberto)|||/items/ceb4045f-526f-4f1b-9aa1-9ac32e034478
Astrup, A. (Arne)|||/items/f6a0e5d3-b236-40b9-a5a3-c380ae3446f2
Saris, W.H.M. (Wim H. M.)|||/items/72a768f2-c225-4139-86ea-e7895ef2a736
MacDonald, I. (Ian)|||/items/c1052005-3adc-4628-8276-f2803549627f
Oppert, J.M. (Jean M.)|||/items/93cbce83-69c1-4820-9e4b-a4003c9c5629
Hansen, T. (Torben)|||/items/de8447d9-7fad-4ca8-9a14-fbe1493a5e91
Pedersen, O. (Oluf)|||/items/7f57f180-6e9b-44fd-914e-e2f48b9c94e7
Sørensen, T.I.A (Thorkild I. A.)|||/items/be862a34-8bb8-4755-ac24-3ed3689e43d0
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638
author_facet Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638
Cruz, R. (R.) de la|||/items/0bf030f8-bd1d-45f1-9a7b-6015e79e71b5
Holst, C. (C.)|||/items/d9318459-9342-49cf-a2da-d6aeb6914a12
Grau, K. (K.)|||/items/dee32024-fa7a-4ab1-8273-3a025e0e73fc
Naranjo, C. (C.)|||/items/9b345f66-f586-434d-84a8-02cfa612745e
Maiz, A. (Alberto)|||/items/ceb4045f-526f-4f1b-9aa1-9ac32e034478
Astrup, A. (Arne)|||/items/f6a0e5d3-b236-40b9-a5a3-c380ae3446f2
Saris, W.H.M. (Wim H. M.)|||/items/72a768f2-c225-4139-86ea-e7895ef2a736
MacDonald, I. (Ian)|||/items/c1052005-3adc-4628-8276-f2803549627f
Oppert, J.M. (Jean M.)|||/items/93cbce83-69c1-4820-9e4b-a4003c9c5629
Hansen, T. (Torben)|||/items/de8447d9-7fad-4ca8-9a14-fbe1493a5e91
Pedersen, O. (Oluf)|||/items/7f57f180-6e9b-44fd-914e-e2f48b9c94e7
Sørensen, T.I.A (Thorkild I. A.)|||/items/be862a34-8bb8-4755-ac24-3ed3689e43d0
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author_role author
author2 Cruz, R. (R.) de la|||/items/0bf030f8-bd1d-45f1-9a7b-6015e79e71b5
Holst, C. (C.)|||/items/d9318459-9342-49cf-a2da-d6aeb6914a12
Grau, K. (K.)|||/items/dee32024-fa7a-4ab1-8273-3a025e0e73fc
Naranjo, C. (C.)|||/items/9b345f66-f586-434d-84a8-02cfa612745e
Maiz, A. (Alberto)|||/items/ceb4045f-526f-4f1b-9aa1-9ac32e034478
Astrup, A. (Arne)|||/items/f6a0e5d3-b236-40b9-a5a3-c380ae3446f2
Saris, W.H.M. (Wim H. M.)|||/items/72a768f2-c225-4139-86ea-e7895ef2a736
MacDonald, I. (Ian)|||/items/c1052005-3adc-4628-8276-f2803549627f
Oppert, J.M. (Jean M.)|||/items/93cbce83-69c1-4820-9e4b-a4003c9c5629
Hansen, T. (Torben)|||/items/de8447d9-7fad-4ca8-9a14-fbe1493a5e91
Pedersen, O. (Oluf)|||/items/7f57f180-6e9b-44fd-914e-e2f48b9c94e7
Sørensen, T.I.A (Thorkild I. A.)|||/items/be862a34-8bb8-4755-ac24-3ed3689e43d0
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Genome wide association
Childhood obesity
Eating behavior
Food intake
Body fat
Polymorphisms
Insulin
Mutations
topic Genome wide association
Childhood obesity
Eating behavior
Food intake
Body fat
Polymorphisms
Insulin
Mutations
description INTRODUCTION: The melanocortin system plays an important role in energy homeostasis. Mice genetically deficient in the melanocortin-3 receptor gene have a normal body weight with increased body fat, mild hypophagia compared to wild-type mice. In humans, Thr6Lys and Val81Ile variants of the melanocortin-3 receptor gene (MC3R) have been associated with childhood obesity, higher BMI Z-score and elevated body fat percentage compared to non-carriers. The aim of this study is to assess the association in adults between allelic variants of MC3R with weight loss induced by energy-restricted diets. SUBJECTS AND METHODS: This research is based on the NUGENOB study, a trial conducted to assess weight loss during a 10-week dietary intervention involving two different hypo-energetic (high-fat and low-fat) diets. A total of 760 obese patients were genotyped for 10 single nucleotide polymorphisms covering the single exon of MC3R gene and its flanking regions, including the missense variants Thr6Lys and Val81Ile. Linear mixed models and haplotype-based analysis were carried out to assess the potential association between genetic polymorphisms and differential weight loss, fat mass loss, waist change and resting energy expenditure changes. RESULTS: No differences in drop-out rate were found by MC3R genotypes. The rs6014646 polymorphism was significantly associated with weight loss using co-dominant (p = 0.04) and dominant models (p = 0.03). These p-values were not statistically significant after strict control for multiple testing. Haplotype-based multivariate analysis using permutations showed that rs3827103-rs1543873 (p = 0.06), rs6014646-rs6024730 (p = 0.05) and rs3746619-rs3827103 (p = 0.10) displayed near-statistical significant results in relation to weight loss. No other significant associations or gene*diet interactions were detected for weight loss, fat mass loss, waist change and resting energy expenditure changes. CONCLUSION: The study provided overall sufficient evidence to support that there is no major effect of genetic variants of MC3R and differential weight loss after a 10-week dietary intervention with hypo-energetic diets in obese Europeans.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01
2011
2011-01-01
2013
2013-03-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/28170
url https://hdl.handle.net/10171/28170
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Sciences
publisher.none.fl_str_mv Public Library of Sciences
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
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