Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica

Aquatic bryophytes are frequently used as bioindicators of water quality in rivers. Poor water quality increases physiological stress in moss, and stress levels can be estimated by measuring the concentrations of photosynthetic pigments and by calculating pigmentary indices. However, studies collect...

Descripción completa

Detalles Bibliográficos
Autores: Villares Pazos, Rubén, Real Rodríguez, Carlos, Vázquez Castro, María Dolores
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/34720
Acceso en línea:http://hdl.handle.net/10347/34720
Access Level:acceso abierto
id ES_9d34b350fda6418bdfb2ef55375de9c8
oai_identifier_str oai:minerva.usc.gal:10347/34720
network_acronym_str ES
network_name_str España
repository_id_str
spelling Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyreticaVillares Pazos, RubénReal Rodríguez, CarlosVázquez Castro, María DoloresAquatic bryophytes are frequently used as bioindicators of water quality in rivers. Poor water quality increases physiological stress in moss, and stress levels can be estimated by measuring the concentrations of photosynthetic pigments and by calculating pigmentary indices. However, studies collecting many samples in a short time may need to store them until analysis. In the meantime, the pigments may suffer some degradation. Given the lack of studies on this problem for aquatic bryophytes, this one investigates the effect of three storage methods (refrigerated at 6 °C, frozen at − 20 °C and dried at room temperature) on the concentrations of chlorophyll a, chlorophyll b, pheophytin a and the following ratios: chlorophyll a/chlorophyll b, D430/D410, D665/D665a, D430/D665 and D480/D665 (where Dx is the absorbance at x nm) in the aquatic moss Fontinalis antipyretica. The results showed that refrigeration was the most suitable method, freezing might be suitable for some parameters, and drying was inadequate for this purpose. We recommend that all studies on photosynthetic pigments detail the time elapsed from collection to sample analysis, as well as the storage method used.SpringerUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10347/34720reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTM2015-70578-P ESTANDARIZACION Y OPTIMIZACION DEL EMPLEO DE BRIOFITOS PARA LA BIOMONITORIZACION PASIVA Y ACTIVA DE LA CALIDAD DE AGUAS CONTINENTALES. ELABORACION DE PROTOCOLOSopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/347202026-06-15T12:47:27Z
dc.title.none.fl_str_mv Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
title Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
spellingShingle Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
Villares Pazos, Rubén
title_short Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
title_full Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
title_fullStr Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
title_full_unstemmed Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
title_sort Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
dc.creator.none.fl_str_mv Villares Pazos, Rubén
Real Rodríguez, Carlos
Vázquez Castro, María Dolores
author Villares Pazos, Rubén
author_facet Villares Pazos, Rubén
Real Rodríguez, Carlos
Vázquez Castro, María Dolores
author_role author
author2 Real Rodríguez, Carlos
Vázquez Castro, María Dolores
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Bioloxía Funcional
description Aquatic bryophytes are frequently used as bioindicators of water quality in rivers. Poor water quality increases physiological stress in moss, and stress levels can be estimated by measuring the concentrations of photosynthetic pigments and by calculating pigmentary indices. However, studies collecting many samples in a short time may need to store them until analysis. In the meantime, the pigments may suffer some degradation. Given the lack of studies on this problem for aquatic bryophytes, this one investigates the effect of three storage methods (refrigerated at 6 °C, frozen at − 20 °C and dried at room temperature) on the concentrations of chlorophyll a, chlorophyll b, pheophytin a and the following ratios: chlorophyll a/chlorophyll b, D430/D410, D665/D665a, D430/D665 and D480/D665 (where Dx is the absorbance at x nm) in the aquatic moss Fontinalis antipyretica. The results showed that refrigeration was the most suitable method, freezing might be suitable for some parameters, and drying was inadequate for this purpose. We recommend that all studies on photosynthetic pigments detail the time elapsed from collection to sample analysis, as well as the storage method used.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/34720
url http://hdl.handle.net/10347/34720
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTM2015-70578-P ESTANDARIZACION Y OPTIMIZACION DEL EMPLEO DE BRIOFITOS PARA LA BIOMONITORIZACION PASIVA Y ACTIVA DE LA CALIDAD DE AGUAS CONTINENTALES. ELABORACION DE PROTOCOLOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414723106111488
score 15,812455

Similares en LA Referencia