Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil

13 Páginas.-- 5 Figuras.-- 3 Tablas

Detalles Bibliográficos
Autores: Hungria, Mariangela, O Chueire, Lı́gia Maria de, Gómez-Coca, R. B., Megías, Manuel
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/413069
Acceso en línea:http://hdl.handle.net/10261/413069
https://api.elsevier.com/content/abstract/scopus_id/0034891455
Access Level:acceso abierto
Palabra clave:Bacterial evolution
Nitrogen fixation
Rhizobium
Sinorhizobium fredii
Soyabean
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spelling Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in BrazilHungria, MariangelaO Chueire, Lı́gia Maria deGómez-Coca, R. B.Megías, ManuelBacterial evolutionNitrogen fixationRhizobiumSinorhizobium frediiSoyabean13 Páginas.-- 5 Figuras.-- 3 TablasA survey of soyabean rhizobia was carried out with six Asian and one modern soyabean genotypes as trap hosts. Soyabean seedlings were inoculated with soils from 22 Brazilian field sites, including undisturbed areas or areas traditionally cropped with this legume. A total of 30 fast growing strains, able to establish an effective symbiosis with both types of genotypes, were isolated from 12 of the 22 soils, representing 17% of the population in cropped areas and 24% in undisturbed soils. The bacterial mean generation time varied from 85 to 225 min and after 4 days of growth in YM medium the final pH ranged from 3.7 to 6.9. Although isolated from acid soils, only 37% of the strains were able to grow in TY or YM media at pH 4.0, while 60% were alkaline tolerant (pH 9.5). Most strains produced abundant extracellular polysaccharides (73%), were tolerant to 0.5 M NaCl (60%) and a temperature of 40°C (77%), grew in LB medium (67%) and synthesized melanin (53%). The strains differed in the use of C compounds supplied as sole C sources. The majority of the strains showed an intrinsic resistance to the antibiotics (μg ml<sup>-1</sup>) chloramphenicol (10), erythromycin (50), gentamicin (20), kanamicin (30), rifampicin (20) and tetracycline (10) and to the heavy metals cobalt chloride (0.5 mM) and potassium chromate (0.25 mM). A cluster analysis with 81 morphological and physiological parameters placed the strains from undisturbed soils in the central part of the dendrogram, even when isolated from distant areas, indicating that they had a common background. The relatedness decreased with the use of the soils for agriculture. Most of the strains isolated from cropped soils under conventional tillage were characterised by a high tolerance to stressful conditions and the ability to grow with several C sources, contrary to most of the strains isolated from soils under no-tillage management system. The strains differed from S. fredii in several characteristics and the protein and lipopolysaccharide profiles showed that each strain was unique. Therefore, although soyabean is an exotic plant in Brazil, several indigenous rhizobial strains may also establish an effective symbiosis with this legume. © 2001 Elsevier Science Ltd. All rights reserved.The authors would like to thank Dr. Luiz Antonio de Oliveira (INPA, Manaus, AM), Dr. Milton A. T. Vargas (Embrapa-Cerrados, Planaltina, DF), Dr. Ricardo S. Araujo (Embrapa-Arroz e Feijão, Goiânia, GO), Prof. José Oswaldo Siqueira (UFLA, Lavras, MG) and Dr. Marcio Voss (Embrapa-Trigo, Passo Fundo, RS), for sending soil samples. M. Hungria has received a post-doctoral fellowship to Spain from CNPq (Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico, Brazil). L.M.O. Chueire acknowledges fellowships from CNPq and M. Megı́as a grant from CICYT, Spain. The research group in Brazil is supported by FINEP/CNPq/MCT, PRONEX, Group of Excellence on Nitrogen Fixation. This paper was approved for publication by the Technical Director of Embrapa-Soja (98/98).Peer reviewedElsevierMinistério da Ciência, Tecnologia e Inovação (Brasil)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262001info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/413069https://api.elsevier.com/content/abstract/scopus_id/0034891455reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSoil Biology and Biochemistryhttps://doi.org/10.1016/S0038-0717(01)00040-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4130692026-05-22T06:33:51Z
dc.title.none.fl_str_mv Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
title Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
spellingShingle Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
Hungria, Mariangela
Bacterial evolution
Nitrogen fixation
Rhizobium
Sinorhizobium fredii
Soyabean
title_short Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
title_full Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
title_fullStr Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
title_full_unstemmed Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
title_sort Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil
dc.creator.none.fl_str_mv Hungria, Mariangela
O Chueire, Lı́gia Maria de
Gómez-Coca, R. B.
Megías, Manuel
author Hungria, Mariangela
author_facet Hungria, Mariangela
O Chueire, Lı́gia Maria de
Gómez-Coca, R. B.
Megías, Manuel
author_role author
author2 O Chueire, Lı́gia Maria de
Gómez-Coca, R. B.
Megías, Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministério da Ciência, Tecnologia e Inovação (Brasil)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial evolution
Nitrogen fixation
Rhizobium
Sinorhizobium fredii
Soyabean
topic Bacterial evolution
Nitrogen fixation
Rhizobium
Sinorhizobium fredii
Soyabean
description 13 Páginas.-- 5 Figuras.-- 3 Tablas
publishDate 2001
dc.date.none.fl_str_mv 2001
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/413069
https://api.elsevier.com/content/abstract/scopus_id/0034891455
url http://hdl.handle.net/10261/413069
https://api.elsevier.com/content/abstract/scopus_id/0034891455
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Soil Biology and Biochemistry
https://doi.org/10.1016/S0038-0717(01)00040-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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