Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor

Micro-RNA signatures have emerged as advantageous biomarkers for disease prediction opening the route for the development of more direct and accurate therapies. There is an urgent need for reliable tools which can offer fast, highly sensitive, and selective detection of multiple miRNAs in complex ma...

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Detalles Bibliográficos
Autores: Huertas, César S.|||0000-0002-3100-4034, Fariña, David, Lechuga, Laura M|||0000-0001-5187-5358
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:199186
Acceso en línea:https://ddd.uab.cat/record/199186
https://dx.doi.org/urn:doi:10.1021/acssensors.6b00162
Access Level:acceso abierto
Palabra clave:Biomarker
Cancer
Diagnosis
MicroRNA
MiRNA
Nanophotonic biosensor
Waveguide interferometer
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spelling Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic BiosensorHuertas, César S.|||0000-0002-3100-4034Fariña, DavidLechuga, Laura M|||0000-0001-5187-5358BiomarkerCancerDiagnosisMicroRNAMiRNANanophotonic biosensorWaveguide interferometerMicro-RNA signatures have emerged as advantageous biomarkers for disease prediction opening the route for the development of more direct and accurate therapies. There is an urgent need for reliable tools which can offer fast, highly sensitive, and selective detection of multiple miRNAs in complex matrices as opposed to the conventional techniques. Here, we demonstrate a nanophotonic biosensor with potential multiplexing capabilities based on interferometric bimodal nanowaveguides (BiMW) for ultrasensitive detection of microRNAs in complex media. Concretely, the BiMW biosensor has been employed for the detection and quantification of miR-181a at attomolar concentrations (LOD = 23 aM) directly, and for the first time, in human urine samples of bladder cancer patients with no need for prior sample purification or amplification steps. We demonstrate the extreme selectivity of our methodology for miR-181a detection showing the discrimination of homologous sequences at single nucleotide mismatch and its pre-miRNA. A significant overexpression of miR-181a in bladder cancer patients was appreciated when compared with healthy controls, suggesting the implication of this miRNA in bladder cancer. Our results show that the BiMW biosensor can be used as an ultrasensitive and specific diagnostic tool by the early and fast detection and quantification of microRNAs for the prediction of diseases (such as cancer) with well-defined microRNA signatures. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/199186https://dx.doi.org/urn:doi:10.1021/acssensors.6b00162reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1991862026-06-06T12:50:31Z
dc.title.none.fl_str_mv Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
title Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
spellingShingle Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
Huertas, César S.|||0000-0002-3100-4034
Biomarker
Cancer
Diagnosis
MicroRNA
MiRNA
Nanophotonic biosensor
Waveguide interferometer
title_short Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
title_full Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
title_fullStr Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
title_full_unstemmed Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
title_sort Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
dc.creator.none.fl_str_mv Huertas, César S.|||0000-0002-3100-4034
Fariña, David
Lechuga, Laura M|||0000-0001-5187-5358
author Huertas, César S.|||0000-0002-3100-4034
author_facet Huertas, César S.|||0000-0002-3100-4034
Fariña, David
Lechuga, Laura M|||0000-0001-5187-5358
author_role author
author2 Fariña, David
Lechuga, Laura M|||0000-0001-5187-5358
author2_role author
author
dc.subject.none.fl_str_mv Biomarker
Cancer
Diagnosis
MicroRNA
MiRNA
Nanophotonic biosensor
Waveguide interferometer
topic Biomarker
Cancer
Diagnosis
MicroRNA
MiRNA
Nanophotonic biosensor
Waveguide interferometer
description Micro-RNA signatures have emerged as advantageous biomarkers for disease prediction opening the route for the development of more direct and accurate therapies. There is an urgent need for reliable tools which can offer fast, highly sensitive, and selective detection of multiple miRNAs in complex matrices as opposed to the conventional techniques. Here, we demonstrate a nanophotonic biosensor with potential multiplexing capabilities based on interferometric bimodal nanowaveguides (BiMW) for ultrasensitive detection of microRNAs in complex media. Concretely, the BiMW biosensor has been employed for the detection and quantification of miR-181a at attomolar concentrations (LOD = 23 aM) directly, and for the first time, in human urine samples of bladder cancer patients with no need for prior sample purification or amplification steps. We demonstrate the extreme selectivity of our methodology for miR-181a detection showing the discrimination of homologous sequences at single nucleotide mismatch and its pre-miRNA. A significant overexpression of miR-181a in bladder cancer patients was appreciated when compared with healthy controls, suggesting the implication of this miRNA in bladder cancer. Our results show that the BiMW biosensor can be used as an ultrasensitive and specific diagnostic tool by the early and fast detection and quantification of microRNAs for the prediction of diseases (such as cancer) with well-defined microRNA signatures.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/199186
https://dx.doi.org/urn:doi:10.1021/acssensors.6b00162
url https://ddd.uab.cat/record/199186
https://dx.doi.org/urn:doi:10.1021/acssensors.6b00162
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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