Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy

In this work, we developed a hybrid nanosystem able to simultaneously combine its potential as a photodynamic (PDT) and plasmonic photothermal (PPTT) therapeutic agent to kill malignant cells. To do that, AuNRs were functionalized by means of the layer-by-layer (LbL) assembly technique using alterna...

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Autores: Arellano, Lilia G., Villar Álvarez, Eva M., Velasco Rodríguez, Brenda, Domínguez Arca, Vicente, Prieto Estévez, Gerardo, Cambón Freire, Adriana, Barbosa Fernández, Silvia, Taboada Antelo, Pablo
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/43943
Acceso en línea:https://hdl.handle.net/10347/43943
Access Level:acceso abierto
Palabra clave:Gold nanorods
Layer by layer
Photodynamic therapy
Isocyanine gree
Phothermal therapy
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spelling Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapyArellano, Lilia G.Villar Álvarez, Eva M.Velasco Rodríguez, BrendaDomínguez Arca, VicentePrieto Estévez, GerardoCambón Freire, AdrianaBarbosa Fernández, SilviaTaboada Antelo, PabloGold nanorodsLayer by layerPhotodynamic therapyIsocyanine greePhothermal therapyIn this work, we developed a hybrid nanosystem able to simultaneously combine its potential as a photodynamic (PDT) and plasmonic photothermal (PPTT) therapeutic agent to kill malignant cells. To do that, AuNRs were functionalized by means of the layer-by-layer (LbL) assembly technique using alternating layers of anionic poly(styrene sulfonate) (PSS) and cationic poly-L-lysine (PLL) as polyelectrolytes (PEs), and an outer final layer of hyaluronic acid (HA) to provide the hybrid particles with both sufficient colloidal stability and targeting ability to tumoral cells overexpressing CD44 receptors. To provide the nanoplatform with PDT capabilities, the near-infrared (NIR) sensitive photosensitizer (PS) indocyanine green (ICG) was previously grafted to the PLL PE and assembled on the particle surface coating; in this manner, PSS/PLL-ICG/HA-coated AuNRs hybrid particles were obtained. Then, the conditions for optimized reactive oxygen species (ROS) production under NIR light excitation were elucidated, reaching ca. 80% after 5 min irradiation at 2.0 W/cm2 compared to positive control (H2O2). Since ICG can also absorb near infrared (NIR) light and transforms it by internal conversion into heat under suitable irradiation conditions, which adds to the photothermal plasmonic effect provided by the metallic NP, the heating profiles provided by the present hybrid nanoparticles (NPs) were measured, being observed temperature increments ranging from 9 to 22 °C. The potential contribution of its different components to both PDT and PPTT were deeply analyzed in vitro for intended cancer therapeutics at several power intensities but also at different temperatures with the aim of elucidating the role played by both phototherapies on cell cytotoxicity, the optimal illumination conditions for effective bimodal phototherapy as well as the elucidation of the main cell death mechanism involved. Under optimized conditions and after the administration of 2.5 ·1010 NPs/mL, cell cytotoxicites of up to ca. 70% were determined by combination of PDT + PPTT therapeutic effect.ElsevierUniversidade de Santiago de Compostela. Departamento de Física de Partículas20232023-02-2420232023-02-24journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/43943reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-109517RB-I00 NUEVOS NANOTRANSPORTADORES BIOMIMETICOS YPLATAFORMAS IN VITRO PARA LA VALIDACION EXITOSA DE LA TERAGNOSTICA PARA LA ATEROSCLEROSISAxencia Galega de Innovación Not available ED431C 2022%2F18open accesshttp://purl.org/coar/access_right/c_abf22023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/439432026-06-15T12:47:27Z
dc.title.none.fl_str_mv Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
title Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
spellingShingle Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
Arellano, Lilia G.
Gold nanorods
Layer by layer
Photodynamic therapy
Isocyanine gree
Phothermal therapy
title_short Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
title_full Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
title_fullStr Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
title_full_unstemmed Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
title_sort Light excitation of gold Nanorod-Based hybrid nanoplatforms for simultaneous bimodal phototherapy
dc.creator.none.fl_str_mv Arellano, Lilia G.
Villar Álvarez, Eva M.
Velasco Rodríguez, Brenda
Domínguez Arca, Vicente
Prieto Estévez, Gerardo
Cambón Freire, Adriana
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author Arellano, Lilia G.
author_facet Arellano, Lilia G.
Villar Álvarez, Eva M.
Velasco Rodríguez, Brenda
Domínguez Arca, Vicente
Prieto Estévez, Gerardo
Cambón Freire, Adriana
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author_role author
author2 Villar Álvarez, Eva M.
Velasco Rodríguez, Brenda
Domínguez Arca, Vicente
Prieto Estévez, Gerardo
Cambón Freire, Adriana
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Física de Partículas
dc.subject.none.fl_str_mv Gold nanorods
Layer by layer
Photodynamic therapy
Isocyanine gree
Phothermal therapy
topic Gold nanorods
Layer by layer
Photodynamic therapy
Isocyanine gree
Phothermal therapy
description In this work, we developed a hybrid nanosystem able to simultaneously combine its potential as a photodynamic (PDT) and plasmonic photothermal (PPTT) therapeutic agent to kill malignant cells. To do that, AuNRs were functionalized by means of the layer-by-layer (LbL) assembly technique using alternating layers of anionic poly(styrene sulfonate) (PSS) and cationic poly-L-lysine (PLL) as polyelectrolytes (PEs), and an outer final layer of hyaluronic acid (HA) to provide the hybrid particles with both sufficient colloidal stability and targeting ability to tumoral cells overexpressing CD44 receptors. To provide the nanoplatform with PDT capabilities, the near-infrared (NIR) sensitive photosensitizer (PS) indocyanine green (ICG) was previously grafted to the PLL PE and assembled on the particle surface coating; in this manner, PSS/PLL-ICG/HA-coated AuNRs hybrid particles were obtained. Then, the conditions for optimized reactive oxygen species (ROS) production under NIR light excitation were elucidated, reaching ca. 80% after 5 min irradiation at 2.0 W/cm2 compared to positive control (H2O2). Since ICG can also absorb near infrared (NIR) light and transforms it by internal conversion into heat under suitable irradiation conditions, which adds to the photothermal plasmonic effect provided by the metallic NP, the heating profiles provided by the present hybrid nanoparticles (NPs) were measured, being observed temperature increments ranging from 9 to 22 °C. The potential contribution of its different components to both PDT and PPTT were deeply analyzed in vitro for intended cancer therapeutics at several power intensities but also at different temperatures with the aim of elucidating the role played by both phototherapies on cell cytotoxicity, the optimal illumination conditions for effective bimodal phototherapy as well as the elucidation of the main cell death mechanism involved. Under optimized conditions and after the administration of 2.5 ·1010 NPs/mL, cell cytotoxicites of up to ca. 70% were determined by combination of PDT + PPTT therapeutic effect.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-02-24
2023
2023-02-24
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/43943
url https://hdl.handle.net/10347/43943
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-109517RB-I00 NUEVOS NANOTRANSPORTADORES BIOMIMETICOS YPLATAFORMAS IN VITRO PARA LA VALIDACION EXITOSA DE LA TERAGNOSTICA PARA LA ATEROSCLEROSIS
Axencia Galega de Innovación Not available ED431C 2022%2F18
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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