The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida

Absorption of excessive light by photosymbiotic organisms leads to the production of reactive oxygen species that can damage both symbiont and host. This is highly relevant in sacoglossan sea slugs that host functional chloroplasts ‘stolen’ from their algal foods (kleptoplasts), because of limited r...

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Detalles Bibliográficos
Autores: Cartaxana, Paulo, Morelli, Luca, Jesús, Bruno, Calado, Gonçalo, Calado, Ricardo, Cruz, Sonia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/207268
Acceso en línea:http://hdl.handle.net/10261/207268
Access Level:acceso abierto
Palabra clave:Kleptoplasty
Light stress
Violaxanthin cycle
Photobehaviour
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spelling The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timidaCartaxana, PauloMorelli, LucaJesús, BrunoCalado, GonçaloCalado, RicardoCruz, SoniaKleptoplastyLight stressViolaxanthin cyclePhotobehaviourAbsorption of excessive light by photosymbiotic organisms leads to the production of reactive oxygen species that can damage both symbiont and host. This is highly relevant in sacoglossan sea slugs that host functional chloroplasts ‘stolen’ from their algal foods (kleptoplasts), because of limited repair capacities resulting from the absence of algal nuclear genes. Here, we experimentally demonstrate (i) a host-mediated photoprotection mechanism in the photosynthetic sea slug Elysia timida, characterized by the closure of the parapodia under high irradiance and the reduction of kleptoplast light exposure; and (ii) the activation of a reversible xanthophyll cycle in kleptoplasts, which allows excessive energy to be dissipated. The described mechanisms reduce photoinactivation under high irradiance. We conclude that both host-mediated behavioural and plastid-based physiological photoprotective mechanisms can mitigate oxidative stress induced by high light in E. timida. These mechanisms may play an important role in the establishment of long-term photosynthetically active kleptoplasts.This work was supported by Programa Investigador, contract IF/00899/2014; by R&D project HULK - PTDC/BIA-ANM/4622/2014 – POCI-01-0145-FEDER-016754 funded by Fundo Europeu de Desenvolvimento Regional (FEDER), through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE); and by support to CESAM through UID/AMB/50017/2019 (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior, FCT/MCTES). P.C. was funded by FCT in the scope of the framework contract foreseen in numbers 4, 5 and 6 of article 23 of the Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.Peer reviewedCompany of BiologistsEuropean CommissionFundação para a Ciência e a Tecnologia (Portugal)Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/207268reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1242/jeb.202580Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2072682026-05-22T06:33:51Z
dc.title.none.fl_str_mv The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
title The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
spellingShingle The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
Cartaxana, Paulo
Kleptoplasty
Light stress
Violaxanthin cycle
Photobehaviour
title_short The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
title_full The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
title_fullStr The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
title_full_unstemmed The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
title_sort The photon menace: kleptoplast protection in the photosynthetic sea slug Elysia timida
dc.creator.none.fl_str_mv Cartaxana, Paulo
Morelli, Luca
Jesús, Bruno
Calado, Gonçalo
Calado, Ricardo
Cruz, Sonia
author Cartaxana, Paulo
author_facet Cartaxana, Paulo
Morelli, Luca
Jesús, Bruno
Calado, Gonçalo
Calado, Ricardo
Cruz, Sonia
author_role author
author2 Morelli, Luca
Jesús, Bruno
Calado, Gonçalo
Calado, Ricardo
Cruz, Sonia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Fundação para a Ciência e a Tecnologia (Portugal)
Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Kleptoplasty
Light stress
Violaxanthin cycle
Photobehaviour
topic Kleptoplasty
Light stress
Violaxanthin cycle
Photobehaviour
description Absorption of excessive light by photosymbiotic organisms leads to the production of reactive oxygen species that can damage both symbiont and host. This is highly relevant in sacoglossan sea slugs that host functional chloroplasts ‘stolen’ from their algal foods (kleptoplasts), because of limited repair capacities resulting from the absence of algal nuclear genes. Here, we experimentally demonstrate (i) a host-mediated photoprotection mechanism in the photosynthetic sea slug Elysia timida, characterized by the closure of the parapodia under high irradiance and the reduction of kleptoplast light exposure; and (ii) the activation of a reversible xanthophyll cycle in kleptoplasts, which allows excessive energy to be dissipated. The described mechanisms reduce photoinactivation under high irradiance. We conclude that both host-mediated behavioural and plastid-based physiological photoprotective mechanisms can mitigate oxidative stress induced by high light in E. timida. These mechanisms may play an important role in the establishment of long-term photosynthetically active kleptoplasts.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/207268
url http://hdl.handle.net/10261/207268
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1242/jeb.202580
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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