A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells

[EN] Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pat...

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Detalles Bibliográficos
Autores: Truchado, Daniel A., Rincón, Sara, Zurita, Lucia, Tomé-Amat, Jaime, Lorz, Corina, Ponz, Fernando, Juárez Molina, María
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/207482
Acceso en línea:https://riunet.upv.es/handle/10251/207482
Access Level:acceso abierto
Palabra clave:Turnip mosaic virus
Virus-Like Particle (VLP)
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticle (VNP)
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spelling A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer CellsTruchado, Daniel A.Rincón, SaraZurita, LuciaTomé-Amat, JaimeLorz, CorinaPonz, FernandoJuárez Molina, MaríaTurnip mosaic virusVirus-Like Particle (VLP)Protein AZ domainCetuximabSquamous cell carcinomaViral nanoparticle (VNP)[EN] Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP-cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.This work was conceived as transversal to efforts experimentally aimed to TuMV nanobiotechnological functionalizations. Consequently, it has benefited from different grants obtained throughout the years. These are P2018/BAA-4574, COV20/00114, and PanGreen-CM from the Comunidad de Madrid; RTA2015-00017-00-00 from INIA; and ARIMNet-2 618127, an ERANet project. DAT was supported by a Margarita Salas postdoctoral grant funded by the Spanish Ministry of Universities and UCM (CT31/21). SR was funded by a contract under grant P2018/BAA-4574 from the Comunidad de Madrid. The CBGP was granted Severo Ochoa Distinctions of Excellence by the Spanish Ministry of Science and Innovation (SEV- 2016-0672 and CEX2020-000999-S).MDPIComunidad de MadridEuropean CommissionInstituto de Salud Carlos IIIAgencia Estatal de InvestigaciónUniversidad Complutense de MadridMinisterio de Ciencia, Innovación y UniversidadesInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-04-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/207482reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 FP7 618127 Coordination of the Agricultural Research in the MediterraneanUniversidad Católica del Maule, Chile https://doi.org/10.13039/501100002911 CT31%2F21Caja de Ahorros del Mediterráneo https://doi.org/10.13039/100012818 P2018%2FBAA-4574Instituto Nacional de Innovación Agraria, Perú https://doi.org/10.13039/100007652 RTA2015-00017-00-00Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 COV20%2F00114Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 Centros de Excelencia Severo Ochoa 2016 SEV-2016-0672 CENTRO DE BIOTECNOLOGIA Y GENOMICA DE PLANTASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Centros de Excelencia Severo Ochoa 2020 CEX2020-000999-S CENTRO DE BIOTECNOLOGIA Y GENOMICA DE PLANTASopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2074822026-06-13T07:49:27Z
dc.title.none.fl_str_mv A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
title A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
spellingShingle A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
Truchado, Daniel A.
Turnip mosaic virus
Virus-Like Particle (VLP)
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticle (VNP)
title_short A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
title_full A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
title_fullStr A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
title_full_unstemmed A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
title_sort A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells
dc.creator.none.fl_str_mv Truchado, Daniel A.
Rincón, Sara
Zurita, Lucia
Tomé-Amat, Jaime
Lorz, Corina
Ponz, Fernando
Juárez Molina, María
author Truchado, Daniel A.
author_facet Truchado, Daniel A.
Rincón, Sara
Zurita, Lucia
Tomé-Amat, Jaime
Lorz, Corina
Ponz, Fernando
Juárez Molina, María
author_role author
author2 Rincón, Sara
Zurita, Lucia
Tomé-Amat, Jaime
Lorz, Corina
Ponz, Fernando
Juárez Molina, María
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
European Commission
Instituto de Salud Carlos III
Agencia Estatal de Investigación
Universidad Complutense de Madrid
Ministerio de Ciencia, Innovación y Universidades
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Turnip mosaic virus
Virus-Like Particle (VLP)
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticle (VNP)
topic Turnip mosaic virus
Virus-Like Particle (VLP)
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticle (VNP)
description [EN] Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP-cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/207482
url https://riunet.upv.es/handle/10251/207482
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 FP7 618127 Coordination of the Agricultural Research in the Mediterranean
Universidad Católica del Maule, Chile https://doi.org/10.13039/501100002911 CT31%2F21
Caja de Ahorros del Mediterráneo https://doi.org/10.13039/100012818 P2018%2FBAA-4574
Instituto Nacional de Innovación Agraria, Perú https://doi.org/10.13039/100007652 RTA2015-00017-00-00
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 COV20%2F00114
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 Centros de Excelencia Severo Ochoa 2016 SEV-2016-0672 CENTRO DE BIOTECNOLOGIA Y GENOMICA DE PLANTAS
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Centros de Excelencia Severo Ochoa 2020 CEX2020-000999-S CENTRO DE BIOTECNOLOGIA Y GENOMICA DE PLANTAS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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