Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.

The mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed p...

Descripción completa

Detalles Bibliográficos
Autores: Díaz-Díaz, Covadonga, Fernandez de Manuel, Laura, Jimenez-Carretero, Daniel, Montoya, María Concepción, Clavería, Cristina, Torres, Miguel
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/15254
Acceso en línea:http://hdl.handle.net/20.500.12105/15254
Access Level:acceso abierto
Palabra clave:Cell Differentiation
Animals
Cell Communication
Cell Lineage
Cell Proliferation
Cell Survival
Cell Tracking
Cells, Cultured
Embryo, Mammalian
Gastrulation
Gene Expression Profiling
Germ Layers
Inheritance Patterns
Mice
Mouse Embryonic Stem Cells
Pluripotent Stem Cells
Proto-Oncogene Proteins c-myc
Time Factors
id ES_9c0de2cd91a60a85c9ea807a5ce7cbe2
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/15254
network_acronym_str ES
network_name_str España
repository_id_str
spelling Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.Díaz-Díaz, CovadongaFernandez de Manuel, LauraJimenez-Carretero, DanielMontoya, María ConcepciónClavería, CristinaTorres, MiguelCell DifferentiationAnimalsCell CommunicationCell LineageCell ProliferationCell SurvivalCell TrackingCells, CulturedEmbryo, MammalianGastrulationGene Expression ProfilingGerm LayersInheritance PatternsMiceMouse Embryonic Stem CellsPluripotent Stem CellsProto-Oncogene Proteins c-mycTime FactorsThe mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed pluripotent state. During these events, epiblast cells and ESCs are prone to death, driven by competition between Myc-high cells (winners) and Myc-low cells (losers). Using live tracking of Myc levels, we show that Myc-high ESCs approach the naive pluripotency state, whereas Myc-low ESCs are closer to the differentiation-primed state. In ESC colonies, naive cells eliminate differentiating cells by cell competition, which is determined by a limitation in the time losers are able to survive persistent contact with winners. In the mouse embryo, cell competition promotes pluripotency maintenance by elimination of primed lineages before gastrulation. The mechanism described here is relevant to mammalian embryo development and induced pluripotency.ElsevierMinisterio de Economía, Industria y Competitividad (España)Comunidad de Madrid (España)Ministerio de Educación, Cultura y Deporte (España)Fundación ProCNICMinisterio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)20222022-12-0120172017-09-2520172017-09-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/15254reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/152542026-06-12T12:43:37Z
dc.title.none.fl_str_mv Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
title Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
spellingShingle Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
Díaz-Díaz, Covadonga
Cell Differentiation
Animals
Cell Communication
Cell Lineage
Cell Proliferation
Cell Survival
Cell Tracking
Cells, Cultured
Embryo, Mammalian
Gastrulation
Gene Expression Profiling
Germ Layers
Inheritance Patterns
Mice
Mouse Embryonic Stem Cells
Pluripotent Stem Cells
Proto-Oncogene Proteins c-myc
Time Factors
title_short Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
title_full Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
title_fullStr Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
title_full_unstemmed Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
title_sort Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
dc.creator.none.fl_str_mv Díaz-Díaz, Covadonga
Fernandez de Manuel, Laura
Jimenez-Carretero, Daniel
Montoya, María Concepción
Clavería, Cristina
Torres, Miguel
author Díaz-Díaz, Covadonga
author_facet Díaz-Díaz, Covadonga
Fernandez de Manuel, Laura
Jimenez-Carretero, Daniel
Montoya, María Concepción
Clavería, Cristina
Torres, Miguel
author_role author
author2 Fernandez de Manuel, Laura
Jimenez-Carretero, Daniel
Montoya, María Concepción
Clavería, Cristina
Torres, Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Comunidad de Madrid (España)
Ministerio de Educación, Cultura y Deporte (España)
Fundación ProCNIC
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
dc.subject.none.fl_str_mv Cell Differentiation
Animals
Cell Communication
Cell Lineage
Cell Proliferation
Cell Survival
Cell Tracking
Cells, Cultured
Embryo, Mammalian
Gastrulation
Gene Expression Profiling
Germ Layers
Inheritance Patterns
Mice
Mouse Embryonic Stem Cells
Pluripotent Stem Cells
Proto-Oncogene Proteins c-myc
Time Factors
topic Cell Differentiation
Animals
Cell Communication
Cell Lineage
Cell Proliferation
Cell Survival
Cell Tracking
Cells, Cultured
Embryo, Mammalian
Gastrulation
Gene Expression Profiling
Germ Layers
Inheritance Patterns
Mice
Mouse Embryonic Stem Cells
Pluripotent Stem Cells
Proto-Oncogene Proteins c-myc
Time Factors
description The mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed pluripotent state. During these events, epiblast cells and ESCs are prone to death, driven by competition between Myc-high cells (winners) and Myc-low cells (losers). Using live tracking of Myc levels, we show that Myc-high ESCs approach the naive pluripotency state, whereas Myc-low ESCs are closer to the differentiation-primed state. In ESC colonies, naive cells eliminate differentiating cells by cell competition, which is determined by a limitation in the time losers are able to survive persistent contact with winners. In the mouse embryo, cell competition promotes pluripotency maintenance by elimination of primed lineages before gastrulation. The mechanism described here is relevant to mammalian embryo development and induced pluripotency.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-09-25
2017
2017-09-25
2022
2022-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/15254
url http://hdl.handle.net/20.500.12105/15254
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414624310329344
score 15,811543

Similares en LA Referencia