Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction

Rennin-angiotensin system (RAS) has been involved in sperm function, even so, little is known about the implication of one of the RAS axis formed by Ang-(1-7) (angiotensin-(1-7)) and MAS receptor. Hence, in the present work, we focused on elucidating the function of the MAS receptor in human spermat...

Descripción completa

Detalles Bibliográficos
Autores: Valdivia Palacin, Asier, Cortés Meler, Lorea, Beitia, Maider, Totorikaguena Iturriaga, Lide, Aguirregoitia Marcos, Naiara, Corcóstegui, Beatriz, Casis Sáenz, Luis, Matorras Weinig, José Roberto, Irazusta Astiazaran, Jon, Aguirregoitia Marcos, Ekaitz
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/71540
Acceso en línea:http://hdl.handle.net/10810/71540
Access Level:acceso abierto
Palabra clave:angiotensin-(1-7)
sperm motility
acrosome reaction
MAS receptor
id ES_9c09c9380bbf1b0bbf7b385eddbb612a
oai_identifier_str oai:addi.ehu.eus:10810/71540
network_acronym_str ES
network_name_str España
repository_id_str
spelling Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reactionValdivia Palacin, AsierCortés Meler, LoreaBeitia, MaiderTotorikaguena Iturriaga, LideAguirregoitia Marcos, NaiaraCorcóstegui, BeatrizCasis Sáenz, LuisMatorras Weinig, José RobertoIrazusta Astiazaran, JonAguirregoitia Marcos, Ekaitzangiotensin-(1-7)sperm motilityacrosome reactionMAS receptorRennin-angiotensin system (RAS) has been involved in sperm function, even so, little is known about the implication of one of the RAS axis formed by Ang-(1-7) (angiotensin-(1-7)) and MAS receptor. Hence, in the present work, we focused on elucidating the function of the MAS receptor in human spermatozoa. We analyzed the expression and localization of MAS receptor in human spermatozoa and we observed if its activation is able to modulate the sperm motility of normal motility and/or asthenozoospermic patients, as well as, the acrosome reaction of the spermatozoa. MAS receptor is present in human mature spermatozoa, not only at the mRNA level but also at protein level. MAS is localized at the acrosome region, as well as, in the tail of spermatozoa. The sperm incubation with MAS agonist Ang-(1-7) activates at dose-dependent manner the PI3K/AKT pathway (P < 0.01 vs control) and improves the motility of asthenozoospermic patients (P < 0.01 vs control), which is blocked by the specific antagonist (A779) (P < 0.01), but it do not modulate the acrosome reaction. These findings suggest that the ACE2/Ang-(1-7)/Mas axis may be a useful biochemical tool for the treatment of male infertility related to sperm mobility.This work received financial support from the University of the Basque Country (Grants GIU14/26 and GIU17/19). L.T. acknowledges the financial support given by University of the Basque Country (PIF15/149)Bioscientifica202520252020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/71540reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1530/REP-19-0274info:eu-repo/semantics/openAccess© 2020 Society for Reproduction and Fertilityoai:addi.ehu.eus:10810/715402026-06-18T09:23:17Z
dc.title.none.fl_str_mv Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
title Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
spellingShingle Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
Valdivia Palacin, Asier
angiotensin-(1-7)
sperm motility
acrosome reaction
MAS receptor
title_short Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
title_full Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
title_fullStr Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
title_full_unstemmed Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
title_sort Role of Angiotensin-(1-7) via MAS receptor in human sperm motility and acrosome reaction
dc.creator.none.fl_str_mv Valdivia Palacin, Asier
Cortés Meler, Lorea
Beitia, Maider
Totorikaguena Iturriaga, Lide
Aguirregoitia Marcos, Naiara
Corcóstegui, Beatriz
Casis Sáenz, Luis
Matorras Weinig, José Roberto
Irazusta Astiazaran, Jon
Aguirregoitia Marcos, Ekaitz
author Valdivia Palacin, Asier
author_facet Valdivia Palacin, Asier
Cortés Meler, Lorea
Beitia, Maider
Totorikaguena Iturriaga, Lide
Aguirregoitia Marcos, Naiara
Corcóstegui, Beatriz
Casis Sáenz, Luis
Matorras Weinig, José Roberto
Irazusta Astiazaran, Jon
Aguirregoitia Marcos, Ekaitz
author_role author
author2 Cortés Meler, Lorea
Beitia, Maider
Totorikaguena Iturriaga, Lide
Aguirregoitia Marcos, Naiara
Corcóstegui, Beatriz
Casis Sáenz, Luis
Matorras Weinig, José Roberto
Irazusta Astiazaran, Jon
Aguirregoitia Marcos, Ekaitz
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv angiotensin-(1-7)
sperm motility
acrosome reaction
MAS receptor
topic angiotensin-(1-7)
sperm motility
acrosome reaction
MAS receptor
description Rennin-angiotensin system (RAS) has been involved in sperm function, even so, little is known about the implication of one of the RAS axis formed by Ang-(1-7) (angiotensin-(1-7)) and MAS receptor. Hence, in the present work, we focused on elucidating the function of the MAS receptor in human spermatozoa. We analyzed the expression and localization of MAS receptor in human spermatozoa and we observed if its activation is able to modulate the sperm motility of normal motility and/or asthenozoospermic patients, as well as, the acrosome reaction of the spermatozoa. MAS receptor is present in human mature spermatozoa, not only at the mRNA level but also at protein level. MAS is localized at the acrosome region, as well as, in the tail of spermatozoa. The sperm incubation with MAS agonist Ang-(1-7) activates at dose-dependent manner the PI3K/AKT pathway (P < 0.01 vs control) and improves the motility of asthenozoospermic patients (P < 0.01 vs control), which is blocked by the specific antagonist (A779) (P < 0.01), but it do not modulate the acrosome reaction. These findings suggest that the ACE2/Ang-(1-7)/Mas axis may be a useful biochemical tool for the treatment of male infertility related to sperm mobility.
publishDate 2020
dc.date.none.fl_str_mv 2020
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/71540
url http://hdl.handle.net/10810/71540
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1530/REP-19-0274
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
© 2020 Society for Reproduction and Fertility
eu_rights_str_mv openAccess
rights_invalid_str_mv © 2020 Society for Reproduction and Fertility
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414622675599360
score 15,811543

Similares en LA Referencia