Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots

Pulsed electric fields (PEF) can be applied to induce accumulation of bioactive compounds in plant tissues to obtain commodities with health-promoting properties. However, causes of this accumulation are not fully un- derstood as it may result from either an improvement in extraction or an activatio...

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Autores: López-Gámez, Gloria, Elez Martínez, Pedro, Martín Belloso, Olga, Soliva-Fortuny, Robert
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/71668
Acceso en línea:https://doi.org/10.1016/j.postharvbio.2020.111284
http://hdl.handle.net/10459.1/71668
Access Level:acceso abierto
Palabra clave:Pulsed electric fields
Phenolic compounds
Respiration
Phenylalanine ammonia lyase
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spelling Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrotsLópez-Gámez, GloriaElez Martínez, PedroMartín Belloso, OlgaSoliva-Fortuny, RobertPulsed electric fieldsPhenolic compoundsRespirationPhenylalanine ammonia lyasePulsed electric fields (PEF) can be applied to induce accumulation of bioactive compounds in plant tissues to obtain commodities with health-promoting properties. However, causes of this accumulation are not fully un- derstood as it may result from either an improvement in extraction or an activation of stress-related biosynthetic pathways. The objective of this study was to investigate the effects of PEF on the physiological response and elucidating the causes underpinning changes in carrot phenolic contents. Respiration rate, oxidative and pec- tinolytic enzyme activities, synthesis, and content of phenolic compounds were evaluated in PEF-treated (580 J kg−1) carrots after treatment and through storage (12, 24 and 36 h) at 4 °C. The highest production of CO2 and volatile organic compounds was observed 12 h after PEF treatment whereas the largest increases in total phenolic content (80.2 %), p-OH-benzoic (94.7 %), chlorogenic acid (74.9 %) and ferulic acid (52.2 %) occurred 24 h after treatment. Enhanced in phenylalanine ammonia lyase activity indicated that the increase in phenolic compounds may be mainly due to the triggering of biosynthesis pathways instead of structural modifications of the food matrix. Electropermeabilization also induced considerable changes in pectinolytic enzyme activities (increases in pectinmethylesterase and decreases in polygalacturonase) whereas no clear trends were observed for oxidative enzyme activities (peroxidase and polyphenol oxidase) during storage. These results suggest that volatile compounds generation, changes in respiration rate and the biosynthesis of phenolic compounds are induced by PEF application, as a plant defence response to stress.This work was supported by the Spanish Ministry of Economy and Competitiveness (grant AGL2013-44851-R, 2013). Gloria López-Gámez thanks the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR) for the predoctoral grant.Elsevier2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.postharvbio.2020.111284http://hdl.handle.net/10459.1/71668reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MINECO//AGL2013-44851-RVersió postprint del document publicat a: https://doi.org/10.1016/j.postharvbio.2020.111284Postharvest Biology and Technology, 2020, vol. 168, 111284cc-by-nc-nd (c) Elsevier, 2020info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/esoai:repositori.udl.cat:10459.1/716682026-06-24T12:42:17Z
dc.title.none.fl_str_mv Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
title Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
spellingShingle Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
López-Gámez, Gloria
Pulsed electric fields
Phenolic compounds
Respiration
Phenylalanine ammonia lyase
title_short Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
title_full Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
title_fullStr Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
title_full_unstemmed Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
title_sort Pulsed electric fields affect endogenous enzyme activities, respiration and biosynthesis of phenolic compounds in carrots
dc.creator.none.fl_str_mv López-Gámez, Gloria
Elez Martínez, Pedro
Martín Belloso, Olga
Soliva-Fortuny, Robert
author López-Gámez, Gloria
author_facet López-Gámez, Gloria
Elez Martínez, Pedro
Martín Belloso, Olga
Soliva-Fortuny, Robert
author_role author
author2 Elez Martínez, Pedro
Martín Belloso, Olga
Soliva-Fortuny, Robert
author2_role author
author
author
dc.subject.none.fl_str_mv Pulsed electric fields
Phenolic compounds
Respiration
Phenylalanine ammonia lyase
topic Pulsed electric fields
Phenolic compounds
Respiration
Phenylalanine ammonia lyase
description Pulsed electric fields (PEF) can be applied to induce accumulation of bioactive compounds in plant tissues to obtain commodities with health-promoting properties. However, causes of this accumulation are not fully un- derstood as it may result from either an improvement in extraction or an activation of stress-related biosynthetic pathways. The objective of this study was to investigate the effects of PEF on the physiological response and elucidating the causes underpinning changes in carrot phenolic contents. Respiration rate, oxidative and pec- tinolytic enzyme activities, synthesis, and content of phenolic compounds were evaluated in PEF-treated (580 J kg−1) carrots after treatment and through storage (12, 24 and 36 h) at 4 °C. The highest production of CO2 and volatile organic compounds was observed 12 h after PEF treatment whereas the largest increases in total phenolic content (80.2 %), p-OH-benzoic (94.7 %), chlorogenic acid (74.9 %) and ferulic acid (52.2 %) occurred 24 h after treatment. Enhanced in phenylalanine ammonia lyase activity indicated that the increase in phenolic compounds may be mainly due to the triggering of biosynthesis pathways instead of structural modifications of the food matrix. Electropermeabilization also induced considerable changes in pectinolytic enzyme activities (increases in pectinmethylesterase and decreases in polygalacturonase) whereas no clear trends were observed for oxidative enzyme activities (peroxidase and polyphenol oxidase) during storage. These results suggest that volatile compounds generation, changes in respiration rate and the biosynthesis of phenolic compounds are induced by PEF application, as a plant defence response to stress.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.postharvbio.2020.111284
http://hdl.handle.net/10459.1/71668
url https://doi.org/10.1016/j.postharvbio.2020.111284
http://hdl.handle.net/10459.1/71668
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2013-44851-R
Versió postprint del document publicat a: https://doi.org/10.1016/j.postharvbio.2020.111284
Postharvest Biology and Technology, 2020, vol. 168, 111284
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier, 2020
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier, 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
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repository.mail.fl_str_mv
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