Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum

A multilayered complexity of epigenetic and transcriptional regulatory mechanisms underlies neuronal activity-dependent gene transcription. The regulation of RNA Pol II progression along the transcription cycle, from promoter-proximal poising (with RNA Pol II paused at promoter-proximal regions, cha...

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Detalhes bibliográficos
Autores: Galvão-Ferreira, Pedro, Lipinski, Michal, Santos, Fernando, Barco, Ángel, Costa, Rui M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/338128
Acesso em linha:http://hdl.handle.net/10261/338128
Access Level:acceso abierto
Palavra-chave:Learning
Motor skill
RNA Pol II
RPB1
Striatum
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repository_id_str
spelling Skill learning modulates RNA pol II poising at immediate early genes in the adult striatumGalvão-Ferreira, PedroLipinski, MichalSantos, FernandoBarco, ÁngelCosta, Rui M.LearningMotor skillRNA Pol IIRPB1StriatumA multilayered complexity of epigenetic and transcriptional regulatory mechanisms underlies neuronal activity-dependent gene transcription. The regulation of RNA Pol II progression along the transcription cycle, from promoter-proximal poising (with RNA Pol II paused at promoter-proximal regions, characterized by a Ser5P+-rich and Ser2P+-poor RPB1 CTD) to active elongation, has emerged as a major step in transcriptional regulation across several organisms, tissues, and developmental stages, including the nervous system. However, it is not known whether this mechanism is modulated by experience. We investigated the impact of learning a motor skill on RNA Pol II phosphorylation dynamics in the adult mouse striatum. We uncovered that learning modulates the in vivo striatal phosphorylation dynamics of the CTD of the RNA Pol II RPB1 subunit, leading to an increased poising index in trained mice. We found that this modulation occurs at immediate early genes (IEGs), with increased poising of RNA Pol II at both Arc and Fos genes but not at constitutively expressed genes. Furthermore, we confirmed that this was learning dependent, and not just regulated by context or motor activity. These experiments demonstrate a novel phenomenon of learning induced transcriptional modulation in adult brain, which may have implications for our understanding of learning, memory allocation, and consolidation.Peer reviewedSociety for NeuroscienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/338128reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1523/ENEURO.0074-17.2017Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3381282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
title Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
spellingShingle Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
Galvão-Ferreira, Pedro
Learning
Motor skill
RNA Pol II
RPB1
Striatum
title_short Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
title_full Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
title_fullStr Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
title_full_unstemmed Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
title_sort Skill learning modulates RNA pol II poising at immediate early genes in the adult striatum
dc.creator.none.fl_str_mv Galvão-Ferreira, Pedro
Lipinski, Michal
Santos, Fernando
Barco, Ángel
Costa, Rui M.
author Galvão-Ferreira, Pedro
author_facet Galvão-Ferreira, Pedro
Lipinski, Michal
Santos, Fernando
Barco, Ángel
Costa, Rui M.
author_role author
author2 Lipinski, Michal
Santos, Fernando
Barco, Ángel
Costa, Rui M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Learning
Motor skill
RNA Pol II
RPB1
Striatum
topic Learning
Motor skill
RNA Pol II
RPB1
Striatum
description A multilayered complexity of epigenetic and transcriptional regulatory mechanisms underlies neuronal activity-dependent gene transcription. The regulation of RNA Pol II progression along the transcription cycle, from promoter-proximal poising (with RNA Pol II paused at promoter-proximal regions, characterized by a Ser5P+-rich and Ser2P+-poor RPB1 CTD) to active elongation, has emerged as a major step in transcriptional regulation across several organisms, tissues, and developmental stages, including the nervous system. However, it is not known whether this mechanism is modulated by experience. We investigated the impact of learning a motor skill on RNA Pol II phosphorylation dynamics in the adult mouse striatum. We uncovered that learning modulates the in vivo striatal phosphorylation dynamics of the CTD of the RNA Pol II RPB1 subunit, leading to an increased poising index in trained mice. We found that this modulation occurs at immediate early genes (IEGs), with increased poising of RNA Pol II at both Arc and Fos genes but not at constitutively expressed genes. Furthermore, we confirmed that this was learning dependent, and not just regulated by context or motor activity. These experiments demonstrate a novel phenomenon of learning induced transcriptional modulation in adult brain, which may have implications for our understanding of learning, memory allocation, and consolidation.
publishDate 2017
dc.date.none.fl_str_mv 2017
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/338128
url http://hdl.handle.net/10261/338128
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1523/ENEURO.0074-17.2017
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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