On the detection of foodborne viral contamination in edible seaweeds

The consumption of edible seaweed as a sustainable food source is increasing; however, their complex composition and diverse commercial formats pose significant challenges for the detection of pathogens, specifically human enteric viruses. Standardized methods, such as ISO 15216-1:2017 and FDA/BAM,...

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Autores: Alcaine Bellido, Beatriz, Rodrigo Aliaga, Dolores, Randazzo, Walter
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::c1b7c8d48883b344733f2e11eba4f5bc
Acceso en línea:http://hdl.handle.net/10261/426420
https://api.elsevier.com/content/abstract/scopus_id/105032757585
Access Level:acceso abierto
Palabra clave:Edible seaweed
Enteric viruses
Food safety
Norovirus
Standard method
seaweeds
food safety
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spelling On the detection of foodborne viral contamination in edible seaweedsAlcaine Bellido, BeatrizRodrigo Aliaga, DoloresRandazzo, WalterEdible seaweedEnteric virusesFood safetyNorovirusStandard methodseaweedsfood safetyThe consumption of edible seaweed as a sustainable food source is increasing; however, their complex composition and diverse commercial formats pose significant challenges for the detection of pathogens, specifically human enteric viruses. Standardized methods, such as ISO 15216-1:2017 and FDA/BAM, are available for detecting and quantifying viral contamination in a diversity of food matrices, which is crucial to guarantee food safety and implement routine monitoring efforts. However, these methodologies demonstrated limited efficiency when applied to complex food samples, and information on their analytical performance is not available for edible seaweed, yet. In this study, three viral concentration protocols based on ISO or FDA/BAM procedures were comparatively assessed for their analytical performance in detecting human norovirus genogroups I and II (HuNoV GI and GII), hepatitis A virus (HAV), and human astrovirus (HAstV) in three edible seaweed species (Codium spp., Ulva lactuca, and Himanthalia elongata). Among assessed protocols, FDA/BAM method exhibited the highest sensitivity (LoD50% = 5.0 ± 0.8 log GC/25 g) and recovery efficiency (2.2-10.9%), significantly outperforming the alternatively tested ISO-based methods. In addition, the best-performing method was employed to conduct a pilot assessment of viral contamination in commercial seaweeds sold in different formats (fresh, dried, and powdered). The sampling campaign resulted in Ulva lactuca (3/15; 20.0%) and Sargassum muticum (1/15; 6.7%) contaminated with HuNoV GII RNA. Overall, these findings emphasise the need for sensitive and standardized detection methods for enteric viruses in complex food matrices, such as seaweeds.This research was supported by “PEMSTA” (PID2023-149211OB-C32, MCIN/AEI/10.13039/501100011033) project funded to DRA and WR. IATA-CSIC is a Centre of Excellence Severo Ochoa (CEX2021-001189-S MCIN/AEI/10.13039/501100011033).With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S).Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/426420https://api.elsevier.com/content/abstract/scopus_id/105032757585reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149211OB-C32info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-SInternational journal of food microbiologyThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.ijfoodmicro.2026.111733https://doi.org/10.1016/j.ijfoodmicro.2026.111733Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::c1b7c8d48883b344733f2e11eba4f5bc2026-05-22T06:33:51Z
dc.title.none.fl_str_mv On the detection of foodborne viral contamination in edible seaweeds
title On the detection of foodborne viral contamination in edible seaweeds
spellingShingle On the detection of foodborne viral contamination in edible seaweeds
Alcaine Bellido, Beatriz
Edible seaweed
Enteric viruses
Food safety
Norovirus
Standard method
seaweeds
food safety
title_short On the detection of foodborne viral contamination in edible seaweeds
title_full On the detection of foodborne viral contamination in edible seaweeds
title_fullStr On the detection of foodborne viral contamination in edible seaweeds
title_full_unstemmed On the detection of foodborne viral contamination in edible seaweeds
title_sort On the detection of foodborne viral contamination in edible seaweeds
dc.creator.none.fl_str_mv Alcaine Bellido, Beatriz
Rodrigo Aliaga, Dolores
Randazzo, Walter
author Alcaine Bellido, Beatriz
author_facet Alcaine Bellido, Beatriz
Rodrigo Aliaga, Dolores
Randazzo, Walter
author_role author
author2 Rodrigo Aliaga, Dolores
Randazzo, Walter
author2_role author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Edible seaweed
Enteric viruses
Food safety
Norovirus
Standard method
seaweeds
food safety
topic Edible seaweed
Enteric viruses
Food safety
Norovirus
Standard method
seaweeds
food safety
description The consumption of edible seaweed as a sustainable food source is increasing; however, their complex composition and diverse commercial formats pose significant challenges for the detection of pathogens, specifically human enteric viruses. Standardized methods, such as ISO 15216-1:2017 and FDA/BAM, are available for detecting and quantifying viral contamination in a diversity of food matrices, which is crucial to guarantee food safety and implement routine monitoring efforts. However, these methodologies demonstrated limited efficiency when applied to complex food samples, and information on their analytical performance is not available for edible seaweed, yet. In this study, three viral concentration protocols based on ISO or FDA/BAM procedures were comparatively assessed for their analytical performance in detecting human norovirus genogroups I and II (HuNoV GI and GII), hepatitis A virus (HAV), and human astrovirus (HAstV) in three edible seaweed species (Codium spp., Ulva lactuca, and Himanthalia elongata). Among assessed protocols, FDA/BAM method exhibited the highest sensitivity (LoD50% = 5.0 ± 0.8 log GC/25 g) and recovery efficiency (2.2-10.9%), significantly outperforming the alternatively tested ISO-based methods. In addition, the best-performing method was employed to conduct a pilot assessment of viral contamination in commercial seaweeds sold in different formats (fresh, dried, and powdered). The sampling campaign resulted in Ulva lactuca (3/15; 20.0%) and Sargassum muticum (1/15; 6.7%) contaminated with HuNoV GII RNA. Overall, these findings emphasise the need for sensitive and standardized detection methods for enteric viruses in complex food matrices, such as seaweeds.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/426420
https://api.elsevier.com/content/abstract/scopus_id/105032757585
url http://hdl.handle.net/10261/426420
https://api.elsevier.com/content/abstract/scopus_id/105032757585
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149211OB-C32
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-S
International journal of food microbiology
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.ijfoodmicro.2026.111733
https://doi.org/10.1016/j.ijfoodmicro.2026.111733
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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