A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria

Transcriptional regulation allows adaptive and coordinated gene expression, and is essential for life. Processive antitermination systems alter the transcription elongation complex to allow the RNA polymerase to read through multiple terminators in an operon. Here, we describe the discovery of a nov...

ver descrição completa

Detalhes bibliográficos
Autores: Miguel-Arribas, Andrés, Val-Calvo, Jorge, Gago-Córdoba, César, Izquierdo, José M., Abia, David, Wu, Ling Juan, Errington, Jeff, Meijer, Wilfried J.J.
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710434
Acesso em linha:http://hdl.handle.net/10486/710434
https://dx.doi.org/10.1093/nar/gkab360
Access Level:acceso abierto
Palavra-chave:Gene Regulation
Chromatin and Epigenetics
Biología y Biomedicina / Biología
id ES_9bf912eea05bedec8704be3e2a498549
oai_identifier_str oai:repositorio.uam.es:10486/710434
network_acronym_str ES
network_name_str España
repository_id_str
spelling A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteriaMiguel-Arribas, AndrésVal-Calvo, JorgeGago-Córdoba, CésarIzquierdo, José M.Abia, DavidWu, Ling JuanErrington, JeffMeijer, Wilfried J.J.Gene RegulationChromatin and EpigeneticsBiología y Biomedicina / BiologíaTranscriptional regulation allows adaptive and coordinated gene expression, and is essential for life. Processive antitermination systems alter the transcription elongation complex to allow the RNA polymerase to read through multiple terminators in an operon. Here, we describe the discovery of a novel bipartite antitermination system that is widespread among conjugative elements from Gram-positive bacteria, which we named conAn. This system is composed of a large RNA element that exerts antitermination, and a protein that functions as a processivity factor. Besides allowing coordinated expression of very long operons, we show that these systems allow differential expression of genes within an operon, and probably contribute to strict regulation of the conjugation genes by minimizing the effects of spurious transcription. Mechanistic features of the conAn system are likely to decisively influence its host range, with important implications for the spread of antibiotic resistance and virulence genesMinistry of Science and Innovation of the Spanish Government [PID2019 108778GB C21 (AEI/FEDER, EU) to W.J.J.M., RTI2018-098517-B100 to J.M.I.]; Wellcome Investigator grant [209500 to J.E., L.J.W.]; EMBO Shortterm Fellowship [7849]; FEMS research and training grant [FEMS-GO-2018–2019 to A.M.A.]; institutional grantsfrom the ‘Fundacion Ram ´ on Areces’ and ‘Banco de San- ´ tander’ to the Centro de Biolog´ıa Molecular ‘Severo Ochoa’; publication fee by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). Funding for open access charge: Ministry of Economy and Competitiveness of the Spanish Government [PID2019 108778GB C21 (AEI/FEDER, EU) to W.J.J.M.]; URICI of the CSIC (spanish Research Council)Oxford University PressDepartamento de Biología MolecularFacultad de Ciencias20212021-05-17research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710434https://dx.doi.org/10.1093/nar/gkab360reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7104342026-06-23T12:46:27Z
dc.title.none.fl_str_mv A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
title A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
spellingShingle A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
Miguel-Arribas, Andrés
Gene Regulation
Chromatin and Epigenetics
Biología y Biomedicina / Biología
title_short A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
title_full A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
title_fullStr A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
title_full_unstemmed A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
title_sort A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria
dc.creator.none.fl_str_mv Miguel-Arribas, Andrés
Val-Calvo, Jorge
Gago-Córdoba, César
Izquierdo, José M.
Abia, David
Wu, Ling Juan
Errington, Jeff
Meijer, Wilfried J.J.
author Miguel-Arribas, Andrés
author_facet Miguel-Arribas, Andrés
Val-Calvo, Jorge
Gago-Córdoba, César
Izquierdo, José M.
Abia, David
Wu, Ling Juan
Errington, Jeff
Meijer, Wilfried J.J.
author_role author
author2 Val-Calvo, Jorge
Gago-Córdoba, César
Izquierdo, José M.
Abia, David
Wu, Ling Juan
Errington, Jeff
Meijer, Wilfried J.J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Gene Regulation
Chromatin and Epigenetics
Biología y Biomedicina / Biología
topic Gene Regulation
Chromatin and Epigenetics
Biología y Biomedicina / Biología
description Transcriptional regulation allows adaptive and coordinated gene expression, and is essential for life. Processive antitermination systems alter the transcription elongation complex to allow the RNA polymerase to read through multiple terminators in an operon. Here, we describe the discovery of a novel bipartite antitermination system that is widespread among conjugative elements from Gram-positive bacteria, which we named conAn. This system is composed of a large RNA element that exerts antitermination, and a protein that functions as a processivity factor. Besides allowing coordinated expression of very long operons, we show that these systems allow differential expression of genes within an operon, and probably contribute to strict regulation of the conjugation genes by minimizing the effects of spurious transcription. Mechanistic features of the conAn system are likely to decisively influence its host range, with important implications for the spread of antibiotic resistance and virulence genes
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-05-17
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710434
https://dx.doi.org/10.1093/nar/gkab360
url http://hdl.handle.net/10486/710434
https://dx.doi.org/10.1093/nar/gkab360
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414616939888640
score 15,301603

Registos relacionados