In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis.
Parasitic diseases represent a significant global public health concern. Two clinically important parasites of high prevalence rates are Trichinella spiralis and Strongyloides stercoralis. However, the limitations of currently used nematocidal drugs highlight the urgent need for novel treatment appr...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/163244 |
| Acceso en línea: | http://hdl.handle.net/10366/163244 |
| Access Level: | acceso abierto |
| Palabra clave: | nematocidal activity parasitic activity medicinal plants methanol extract Trichinella spiralis Strongyloides venezuelensis 3207.12 Parasitología 2412 Inmunología 2401.12 Parasitología Animal 3209 Farmacología |
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In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis.Rodríguez-Garza, Nancy EGomez-Flores, RicardoQuintanilla-Licea, RamiroElizondo Luévano, Joel HoracioRomo-Sáenz, César IMarín, MiguelSánchez-Montejo, JavierMuro Álvarez, AntonioPeláez Lamamie de C. Arroyo, RafaelLópez Abán, Julionematocidal activityparasitic activitymedicinal plantsmethanol extractTrichinella spiralisStrongyloides venezuelensis3207.12 Parasitología2412 Inmunología2401.12 Parasitología Animal3209 FarmacologíaParasitic diseases represent a significant global public health concern. Two clinically important parasites of high prevalence rates are Trichinella spiralis and Strongyloides stercoralis. However, the limitations of currently used nematocidal drugs highlight the urgent need for novel treatment approaches. The present study investigated the in vitro nematocidal activity of methanol extracts from Amphipterygium adstringens, Artemisia ludoviciana, Cymbopogon citratus, Heterotheca inuloides, Jatropha dioica, Justicia spicigera, Larrea tridentata, Mimosa tenuiflora, Psacalium decompositum, Ruta chalepensis, Semialarium mexicanum, and Smilax aspera against T. spiralis L1 and S. venezuelensis L3 (model for S. stercoralis). Most of the plants showed antiparasitic activity, but R. chalepensis crude methanol extract showed the most potent nematocidal activity against both parasites, with a mean lethal concentration (LC50) of 28.2 µg/mL and a selectivity index (SI) of 22.4 for T. spiralis and an LC50 of 244.8 µg/mL and SI of 2.58 for S. venezuelensis. This extract was further separated into n-hexane, chloroform, and methanol partitions by continuous Soxhlet extractions. The n-hexane partition demonstrated the strongest activity against both parasites, with an LC50 of 147.6 µg/mL and an SI of 7.77 against T. spiralis and an LC50 of 39.2 µg/mL and an SI of 3.77 against S. venezuelensis. LC-MS/MS analysis identified coumarins as the main chemical class (53%), and chalepin represented this partition's most abundant compound (29.9%). Overall, this study confirmed the antiparasitic potential of medicinal plants commonly used in Mexico. In addition, it highlights the possibility of obtaining bioactive compounds from plants like R. chalepensis, or the other plants evaluated in this study, as novel treatments against parasitic diseases.Consejo Nacional de Humanidades, Ciencia y Tecnología (CONHACYT). N.E.R.-G. received a doctoral grant numbered 808132 (CVU: 1006989), J.H.E.-L. received a postdoctoral grant numbered I1200/331/2023 (CVU: 418935), and C.I.R.-S. received a postdoctoral grant numbered 877783 (CVU: 445572). R.Q.-L. is supported by the Program Apoyo a la Investigación Científica y Tecnológica of the Universidad Autónoma de Nuevo León (PAICYTUANL), grant number 307-CN-2022. The Doctoral Fellowship Program of “Junta de Castilla y León” is co-funded by “Fondo Social Europeo” (MM and JS-M ORDEN EDU/601/2020). Instituto de Salud Carlos III grant PI22/01721 financed to A.M, and J.L.-A.; MCIN/AEI/doi 10.13039/501100011033 cofounded by the European Union, grant PID2022-136462NB-I00 financed to A.M; and MCIN/AEI/doi 10.13039/501100011033 co-founded by European Union grant PID2021-127471OB-I00 financed to J.L.-A. and R.P (Principal Researcher).MDPI202520252024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/163244reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésPID2021-127471OB-I00Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1632442026-06-07T06:28:51Z |
| dc.title.none.fl_str_mv |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| title |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| spellingShingle |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. Rodríguez-Garza, Nancy E nematocidal activity parasitic activity medicinal plants methanol extract Trichinella spiralis Strongyloides venezuelensis 3207.12 Parasitología 2412 Inmunología 2401.12 Parasitología Animal 3209 Farmacología |
| title_short |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| title_full |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| title_fullStr |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| title_full_unstemmed |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| title_sort |
In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. |
| dc.creator.none.fl_str_mv |
Rodríguez-Garza, Nancy E Gomez-Flores, Ricardo Quintanilla-Licea, Ramiro Elizondo Luévano, Joel Horacio Romo-Sáenz, César I Marín, Miguel Sánchez-Montejo, Javier Muro Álvarez, Antonio Peláez Lamamie de C. Arroyo, Rafael López Abán, Julio |
| author |
Rodríguez-Garza, Nancy E |
| author_facet |
Rodríguez-Garza, Nancy E Gomez-Flores, Ricardo Quintanilla-Licea, Ramiro Elizondo Luévano, Joel Horacio Romo-Sáenz, César I Marín, Miguel Sánchez-Montejo, Javier Muro Álvarez, Antonio Peláez Lamamie de C. Arroyo, Rafael López Abán, Julio |
| author_role |
author |
| author2 |
Gomez-Flores, Ricardo Quintanilla-Licea, Ramiro Elizondo Luévano, Joel Horacio Romo-Sáenz, César I Marín, Miguel Sánchez-Montejo, Javier Muro Álvarez, Antonio Peláez Lamamie de C. Arroyo, Rafael López Abán, Julio |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
nematocidal activity parasitic activity medicinal plants methanol extract Trichinella spiralis Strongyloides venezuelensis 3207.12 Parasitología 2412 Inmunología 2401.12 Parasitología Animal 3209 Farmacología |
| topic |
nematocidal activity parasitic activity medicinal plants methanol extract Trichinella spiralis Strongyloides venezuelensis 3207.12 Parasitología 2412 Inmunología 2401.12 Parasitología Animal 3209 Farmacología |
| description |
Parasitic diseases represent a significant global public health concern. Two clinically important parasites of high prevalence rates are Trichinella spiralis and Strongyloides stercoralis. However, the limitations of currently used nematocidal drugs highlight the urgent need for novel treatment approaches. The present study investigated the in vitro nematocidal activity of methanol extracts from Amphipterygium adstringens, Artemisia ludoviciana, Cymbopogon citratus, Heterotheca inuloides, Jatropha dioica, Justicia spicigera, Larrea tridentata, Mimosa tenuiflora, Psacalium decompositum, Ruta chalepensis, Semialarium mexicanum, and Smilax aspera against T. spiralis L1 and S. venezuelensis L3 (model for S. stercoralis). Most of the plants showed antiparasitic activity, but R. chalepensis crude methanol extract showed the most potent nematocidal activity against both parasites, with a mean lethal concentration (LC50) of 28.2 µg/mL and a selectivity index (SI) of 22.4 for T. spiralis and an LC50 of 244.8 µg/mL and SI of 2.58 for S. venezuelensis. This extract was further separated into n-hexane, chloroform, and methanol partitions by continuous Soxhlet extractions. The n-hexane partition demonstrated the strongest activity against both parasites, with an LC50 of 147.6 µg/mL and an SI of 7.77 against T. spiralis and an LC50 of 39.2 µg/mL and an SI of 3.77 against S. venezuelensis. LC-MS/MS analysis identified coumarins as the main chemical class (53%), and chalepin represented this partition's most abundant compound (29.9%). Overall, this study confirmed the antiparasitic potential of medicinal plants commonly used in Mexico. In addition, it highlights the possibility of obtaining bioactive compounds from plants like R. chalepensis, or the other plants evaluated in this study, as novel treatments against parasitic diseases. |
| publishDate |
2024 |
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2024 2025 2025 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10366/163244 |
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http://hdl.handle.net/10366/163244 |
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Inglés |
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Inglés |
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PID2021-127471OB-I00 |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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MDPI |
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MDPI |
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