Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements

Background The role of chromosomal arrangements in adaptation is supported by the repeatable clinal variation in inversion frequencies across continents in colonizing species such as Drosophila subobscura. However, there is a lack of knowledge on the genetic variation in genes within inversions, pos...

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Autores: Simões, Pedro, Pascual Berniola, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/133113
Acceso en línea:https://hdl.handle.net/2445/133113
Access Level:acceso abierto
Palabra clave:Drosòfila subobscura
Cromosomes sexuals
Drosophila subobscura
Sex chromosomes
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spelling Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangementsSimões, PedroPascual Berniola, MartaDrosòfila subobscuraCromosomes sexualsDrosophila subobscuraSex chromosomesBackground The role of chromosomal arrangements in adaptation is supported by the repeatable clinal variation in inversion frequencies across continents in colonizing species such as Drosophila subobscura. However, there is a lack of knowledge on the genetic variation in genes within inversions, possibly targets of climatic selection, across a geographic latitudinal gradient. In the present study we analysed four candidate loci for thermal adaptation, located close to the breakpoints, in two chromosomal arrangements of the sex (A) chromosome of Drosophila subobscura with different thermal preferences. Individual chromosomes with A2 (the inverted arrangement considered warm adapted) or AST (the standard ancestral arrangement considered cold adapted) were sequenced across four European localities at varying latitudes, up to ~ 2500 Kms apart. Results Importantly, we found very low differentiation for each specific arrangement across populations as well as no clinal patterns of genomic variation. This suggests wide gene exchange along the cline. Differentiation between the sex chromosome arrangements was significant in the two more proximal regions relative to the AST orientation but not in the distal ones, independently of their location inside or outside the inversion. This can be possibly due to variation in the levels of gene flux and/or selection acting in these regions. Conclusions Gene flow appears to have homogenized the genetic content within-arrangement at a wide geographical scale, despite the expected diverse selective pressures in the specific natural environments of the different populations sampled. It is thus likely that the inversion frequency clines in this species are being maintained by local adaptation in face of gene flow. The differences between arrangements at non-coding regions might be associated with the previously observed differential gene expression in different thermal regimes. Higher resolution genomic scans for individual chromosomal arrangements performed over a large environmental gradient are needed to find the targets of selection and further elucidate the adaptive mechanisms maintaining chromosomal inversion polymorphisms.BioMed Central2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/133113Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1186/s12862-018-1178-1BMC Evolutionary Biology, 2018, vol. 18, p. 60https://doi.org/10.1186/s12862-018-1178-1cc-by (c) Simões, Pedro et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1331132026-05-27T06:46:51Z
dc.title.none.fl_str_mv Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
title Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
spellingShingle Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
Simões, Pedro
Drosòfila subobscura
Cromosomes sexuals
Drosophila subobscura
Sex chromosomes
title_short Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
title_full Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
title_fullStr Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
title_full_unstemmed Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
title_sort Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements
dc.creator.none.fl_str_mv Simões, Pedro
Pascual Berniola, Marta
author Simões, Pedro
author_facet Simões, Pedro
Pascual Berniola, Marta
author_role author
author2 Pascual Berniola, Marta
author2_role author
dc.subject.none.fl_str_mv Drosòfila subobscura
Cromosomes sexuals
Drosophila subobscura
Sex chromosomes
topic Drosòfila subobscura
Cromosomes sexuals
Drosophila subobscura
Sex chromosomes
description Background The role of chromosomal arrangements in adaptation is supported by the repeatable clinal variation in inversion frequencies across continents in colonizing species such as Drosophila subobscura. However, there is a lack of knowledge on the genetic variation in genes within inversions, possibly targets of climatic selection, across a geographic latitudinal gradient. In the present study we analysed four candidate loci for thermal adaptation, located close to the breakpoints, in two chromosomal arrangements of the sex (A) chromosome of Drosophila subobscura with different thermal preferences. Individual chromosomes with A2 (the inverted arrangement considered warm adapted) or AST (the standard ancestral arrangement considered cold adapted) were sequenced across four European localities at varying latitudes, up to ~ 2500 Kms apart. Results Importantly, we found very low differentiation for each specific arrangement across populations as well as no clinal patterns of genomic variation. This suggests wide gene exchange along the cline. Differentiation between the sex chromosome arrangements was significant in the two more proximal regions relative to the AST orientation but not in the distal ones, independently of their location inside or outside the inversion. This can be possibly due to variation in the levels of gene flux and/or selection acting in these regions. Conclusions Gene flow appears to have homogenized the genetic content within-arrangement at a wide geographical scale, despite the expected diverse selective pressures in the specific natural environments of the different populations sampled. It is thus likely that the inversion frequency clines in this species are being maintained by local adaptation in face of gene flow. The differences between arrangements at non-coding regions might be associated with the previously observed differential gene expression in different thermal regimes. Higher resolution genomic scans for individual chromosomal arrangements performed over a large environmental gradient are needed to find the targets of selection and further elucidate the adaptive mechanisms maintaining chromosomal inversion polymorphisms.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/133113
url https://hdl.handle.net/2445/133113
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s12862-018-1178-1
BMC Evolutionary Biology, 2018, vol. 18, p. 60
https://doi.org/10.1186/s12862-018-1178-1
dc.rights.none.fl_str_mv cc-by (c) Simões, Pedro et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Simões, Pedro et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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