Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures

Brominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in...

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Authors: Palau, Jordi|||0000-0001-9492-7306, Trueba-Santiso, Alba|||0000-0001-9730-7321, Yu, Rong, Mortan, Siti Hatijah|||0000-0003-4968-1508, Shouakar-Stash, Orfan, Freedman, David L.|||0000-0001-6778-3706, Wasmund, Kenneth|||0000-0001-6706-7291, Hunkeler, Daniel, Marco Urrea, Ernest|||0000-0002-8033-6553, Rosell, Mònica|||0000-0003-1563-8595
Format: article
Publication Date:2023
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:273363
Online Access:https://ddd.uab.cat/record/273363
https://dx.doi.org/urn:doi:10.1021/acs.est.2c07137
Access Level:Open access
Keyword:Brominated organic compounds
Groundwater contamination
Biodegradation
Organohalide-respiring bacteria
Compound-specific isotope analysis
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spelling Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing culturesPalau, Jordi|||0000-0001-9492-7306Trueba-Santiso, Alba|||0000-0001-9730-7321Yu, RongMortan, Siti Hatijah|||0000-0003-4968-1508Shouakar-Stash, OrfanFreedman, David L.|||0000-0001-6778-3706Wasmund, Kenneth|||0000-0001-6706-7291Hunkeler, DanielMarco Urrea, Ernest|||0000-0002-8033-6553Rosell, Mònica|||0000-0003-1563-8595Brominated organic compoundsGroundwater contaminationBiodegradationOrganohalide-respiring bacteriaCompound-specific isotope analysisBrominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in contaminated sites. This study investigates for the first time dual C-Br isotope fractionation during in vivo biodegradation of 1,2-DBA by two anaerobic enrichment cultures containing organohalide-respiring bacteria (i.e., either Dehalococcoides or Dehalogenimonas). Different ε C values (-1.8 ± 0.2 and -19.2 ± 3.5‰, respectively) were obtained, whereas their respective ε Br values were lower and similar to each other (-1.22 ± 0.08 and -1.2 ± 0.5‰), leading to distinctly different trends (Λ = Δδ 13 C/Δδ 81 Br ≈ ε C /ε Br) in a dual C-Br isotope plot (1.4 ± 0.2 and 12 ± 4, respectively). These results suggest the occurrence of different underlying reaction mechanisms during enzymatic 1,2-DBA transformation, that is, concerted dihaloelimination and nucleophilic substitution (S2-reaction). The strongly pathway-dependent Λ values illustrate the potential of this approach to elucidate the reaction mechanism of 1,2-DBA in the field and to select appropriate ε C values for quantification of biodegradation. The results of this study provide valuable information for future biodegradation studies of 1,2-DBA in contaminated sites. New insights into the reaction mechanisms of microbial reductive dehalogenation of brominated ethanes and information relevant to the application of CSIA in field studies. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/273363https://dx.doi.org/urn:doi:10.1021/acs.est.2c07137reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PID2019-103989RB-100Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 CGL2017-82331-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-070817Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1733open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2733632026-06-06T12:50:31Z
dc.title.none.fl_str_mv Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
title Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
spellingShingle Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
Palau, Jordi|||0000-0001-9492-7306
Brominated organic compounds
Groundwater contamination
Biodegradation
Organohalide-respiring bacteria
Compound-specific isotope analysis
title_short Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
title_full Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
title_fullStr Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
title_full_unstemmed Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
title_sort Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in dehalococcoides - and dehalogenimonas -containing cultures
dc.creator.none.fl_str_mv Palau, Jordi|||0000-0001-9492-7306
Trueba-Santiso, Alba|||0000-0001-9730-7321
Yu, Rong
Mortan, Siti Hatijah|||0000-0003-4968-1508
Shouakar-Stash, Orfan
Freedman, David L.|||0000-0001-6778-3706
Wasmund, Kenneth|||0000-0001-6706-7291
Hunkeler, Daniel
Marco Urrea, Ernest|||0000-0002-8033-6553
Rosell, Mònica|||0000-0003-1563-8595
author Palau, Jordi|||0000-0001-9492-7306
author_facet Palau, Jordi|||0000-0001-9492-7306
Trueba-Santiso, Alba|||0000-0001-9730-7321
Yu, Rong
Mortan, Siti Hatijah|||0000-0003-4968-1508
Shouakar-Stash, Orfan
Freedman, David L.|||0000-0001-6778-3706
Wasmund, Kenneth|||0000-0001-6706-7291
Hunkeler, Daniel
Marco Urrea, Ernest|||0000-0002-8033-6553
Rosell, Mònica|||0000-0003-1563-8595
author_role author
author2 Trueba-Santiso, Alba|||0000-0001-9730-7321
Yu, Rong
Mortan, Siti Hatijah|||0000-0003-4968-1508
Shouakar-Stash, Orfan
Freedman, David L.|||0000-0001-6778-3706
Wasmund, Kenneth|||0000-0001-6706-7291
Hunkeler, Daniel
Marco Urrea, Ernest|||0000-0002-8033-6553
Rosell, Mònica|||0000-0003-1563-8595
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Brominated organic compounds
Groundwater contamination
Biodegradation
Organohalide-respiring bacteria
Compound-specific isotope analysis
topic Brominated organic compounds
Groundwater contamination
Biodegradation
Organohalide-respiring bacteria
Compound-specific isotope analysis
description Brominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in contaminated sites. This study investigates for the first time dual C-Br isotope fractionation during in vivo biodegradation of 1,2-DBA by two anaerobic enrichment cultures containing organohalide-respiring bacteria (i.e., either Dehalococcoides or Dehalogenimonas). Different ε C values (-1.8 ± 0.2 and -19.2 ± 3.5‰, respectively) were obtained, whereas their respective ε Br values were lower and similar to each other (-1.22 ± 0.08 and -1.2 ± 0.5‰), leading to distinctly different trends (Λ = Δδ 13 C/Δδ 81 Br ≈ ε C /ε Br) in a dual C-Br isotope plot (1.4 ± 0.2 and 12 ± 4, respectively). These results suggest the occurrence of different underlying reaction mechanisms during enzymatic 1,2-DBA transformation, that is, concerted dihaloelimination and nucleophilic substitution (S2-reaction). The strongly pathway-dependent Λ values illustrate the potential of this approach to elucidate the reaction mechanism of 1,2-DBA in the field and to select appropriate ε C values for quantification of biodegradation. The results of this study provide valuable information for future biodegradation studies of 1,2-DBA in contaminated sites. New insights into the reaction mechanisms of microbial reductive dehalogenation of brominated ethanes and information relevant to the application of CSIA in field studies.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/273363
https://dx.doi.org/urn:doi:10.1021/acs.est.2c07137
url https://ddd.uab.cat/record/273363
https://dx.doi.org/urn:doi:10.1021/acs.est.2c07137
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PID2019-103989RB-100
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 CGL2017-82331-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-070817
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1733
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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