Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults

The inflammatory process plays a crucial role in frailty syndrome, which can appear in middle age and is associated with a poor health outcome. Consequently, gerontologists recommend screening inflammatory biomarkers in middle-aged adults to detect frailty and, therefore, prevent chronic diseases an...

Descripción completa

Detalles Bibliográficos
Autores: Crooke Álvarez, Almudena, Martínez Alberquilla, Irene, García Montero, María, Rico Del Viejo, Laura, Ruiz Alcocer, Javier, Madrid Costa, David
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7536
Acceso en línea:https://hdl.handle.net/20.500.14352/7536
Access Level:acceso abierto
Palabra clave:617.7-089.243
617.77-002
Inflammaging
Inflammasome
Mucin
Ectonucleotidase
Ocular frailty
Bioquímica (Medicina)
Oftalmología
Lentes de contacto
3201.09 Oftalmología
3311.11 Instrumentos ópticos
id ES_9bbb8318c30e439aaeeedceafe7293fe
oai_identifier_str oai:docta.ucm.es:20.500.14352/7536
network_acronym_str ES
network_name_str España
repository_id_str
spelling Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adultsCrooke Álvarez, AlmudenaMartínez Alberquilla, IreneGarcía Montero, MaríaRico Del Viejo, LauraRuiz Alcocer, JavierMadrid Costa, David617.7-089.243617.77-002InflammagingInflammasomeMucinEctonucleotidaseOcular frailtyBioquímica (Medicina)OftalmologíaLentes de contacto3201.09 Oftalmología3311.11 Instrumentos ópticosThe inflammatory process plays a crucial role in frailty syndrome, which can appear in middle age and is associated with a poor health outcome. Consequently, gerontologists recommend screening inflammatory biomarkers in middle-aged adults to detect frailty and, therefore, prevent chronic diseases and mortality. External factors could be a risk factor for frailty because they can generate and extend the inflammatory process. For these reasons, we analysed the effect of long-term contact lens wear on mRNA level of genes linked to inflammation (IL-6, NLRP3, NK1R, CD73, MUC16 and TRPV1 genes) in conjunctival cells of middle-aged individuals, by quantitative PCR. Middle-aged contact lens wearers presented a significant increase of NLRP3 and MUC16 mRNA level as well as a decrease of CD73 mRNA level, in comparison with non-contact lens wearers. Additionally, we checked for a potential correlation between these transcript levels and clinical changes of the participants’ ocular surface. Unlike molecular analysis, clinical examination fails to detect inflammation in contact lens wearers. These data suggest that long-term contact lens wear could trigger an inflammatory response in middle age orchestrated by NLRP3 inflammasome and modulated by CD73 and MUC16 proteins. Further studies are needed to confirm our gene expression findings at the protein level as well as to investigate the potential role of long-term CL wear in the onset of ocular frailty.ElsevierUniversidad Complutense de Madrid20202020-11-1320202020-11-13journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7536reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/75362026-06-02T12:44:21Z
dc.title.none.fl_str_mv Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
title Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
spellingShingle Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
Crooke Álvarez, Almudena
617.7-089.243
617.77-002
Inflammaging
Inflammasome
Mucin
Ectonucleotidase
Ocular frailty
Bioquímica (Medicina)
Oftalmología
Lentes de contacto
3201.09 Oftalmología
3311.11 Instrumentos ópticos
title_short Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
title_full Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
title_fullStr Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
title_full_unstemmed Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
title_sort Impact of contact lens wear on NLRP3 gene expression: Implications for ocular frailty in middle-aged adults
dc.creator.none.fl_str_mv Crooke Álvarez, Almudena
Martínez Alberquilla, Irene
García Montero, María
Rico Del Viejo, Laura
Ruiz Alcocer, Javier
Madrid Costa, David
author Crooke Álvarez, Almudena
author_facet Crooke Álvarez, Almudena
Martínez Alberquilla, Irene
García Montero, María
Rico Del Viejo, Laura
Ruiz Alcocer, Javier
Madrid Costa, David
author_role author
author2 Martínez Alberquilla, Irene
García Montero, María
Rico Del Viejo, Laura
Ruiz Alcocer, Javier
Madrid Costa, David
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 617.7-089.243
617.77-002
Inflammaging
Inflammasome
Mucin
Ectonucleotidase
Ocular frailty
Bioquímica (Medicina)
Oftalmología
Lentes de contacto
3201.09 Oftalmología
3311.11 Instrumentos ópticos
topic 617.7-089.243
617.77-002
Inflammaging
Inflammasome
Mucin
Ectonucleotidase
Ocular frailty
Bioquímica (Medicina)
Oftalmología
Lentes de contacto
3201.09 Oftalmología
3311.11 Instrumentos ópticos
description The inflammatory process plays a crucial role in frailty syndrome, which can appear in middle age and is associated with a poor health outcome. Consequently, gerontologists recommend screening inflammatory biomarkers in middle-aged adults to detect frailty and, therefore, prevent chronic diseases and mortality. External factors could be a risk factor for frailty because they can generate and extend the inflammatory process. For these reasons, we analysed the effect of long-term contact lens wear on mRNA level of genes linked to inflammation (IL-6, NLRP3, NK1R, CD73, MUC16 and TRPV1 genes) in conjunctival cells of middle-aged individuals, by quantitative PCR. Middle-aged contact lens wearers presented a significant increase of NLRP3 and MUC16 mRNA level as well as a decrease of CD73 mRNA level, in comparison with non-contact lens wearers. Additionally, we checked for a potential correlation between these transcript levels and clinical changes of the participants’ ocular surface. Unlike molecular analysis, clinical examination fails to detect inflammation in contact lens wearers. These data suggest that long-term contact lens wear could trigger an inflammatory response in middle age orchestrated by NLRP3 inflammasome and modulated by CD73 and MUC16 proteins. Further studies are needed to confirm our gene expression findings at the protein level as well as to investigate the potential role of long-term CL wear in the onset of ocular frailty.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-11-13
2020
2020-11-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7536
url https://hdl.handle.net/20.500.14352/7536
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414579664060416
score 15,300719

Similares en LA Referencia