Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis

16 páginas, 6 figuras, 3 tablas.

Detalles Bibliográficos
Autores: Arteche-Villasol, Noive, Gutiérrez-Expósito, Daniel, Elguezabal, N., Sevilla, I.A., Vallejo, R., Espinosa Cerrato, José, Ferreras, Mª del Carmen, Benavides, Julio, Pérez Pérez, Valentín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/271708
Acceso en línea:http://hdl.handle.net/10261/271708
Access Level:acceso abierto
Palabra clave:Vaccination
Paratuberculosis
Tuberculosis
HIMB
Adjuvant
Cross-protection
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oai_identifier_str oai:digital.csic.es:10261/271708
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
title Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
spellingShingle Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
Arteche-Villasol, Noive
Vaccination
Paratuberculosis
Tuberculosis
HIMB
Adjuvant
Cross-protection
title_short Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
title_full Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
title_fullStr Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
title_full_unstemmed Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
title_sort Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
dc.creator.none.fl_str_mv Arteche-Villasol, Noive
Gutiérrez-Expósito, Daniel
Elguezabal, N.
Sevilla, I.A.
Vallejo, R.
Espinosa Cerrato, José
Ferreras, Mª del Carmen
Benavides, Julio
Pérez Pérez, Valentín
author Arteche-Villasol, Noive
author_facet Arteche-Villasol, Noive
Gutiérrez-Expósito, Daniel
Elguezabal, N.
Sevilla, I.A.
Vallejo, R.
Espinosa Cerrato, José
Ferreras, Mª del Carmen
Benavides, Julio
Pérez Pérez, Valentín
author_role author
author2 Gutiérrez-Expósito, Daniel
Elguezabal, N.
Sevilla, I.A.
Vallejo, R.
Espinosa Cerrato, José
Ferreras, Mª del Carmen
Benavides, Julio
Pérez Pérez, Valentín
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Junta de Castilla y León
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Arteche Villasol, Noive [0000-0001-5793-9578]
Gutiérrez Expósito, Daniel [0000-0002-7683-623X]
Espinosa Cerrato, José [0000-0002-9036-1402]
Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]
Benavides, Julio [0000-0001-9706-100X]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Vaccination
Paratuberculosis
Tuberculosis
HIMB
Adjuvant
Cross-protection
topic Vaccination
Paratuberculosis
Tuberculosis
HIMB
Adjuvant
Cross-protection
description 16 páginas, 6 figuras, 3 tablas.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/271708
url http://hdl.handle.net/10261/271708
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MINECO//AGL2015-66540-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00
info:eu-repo/grantAgreement/INIA//RTA 2017-00089-00-00
info:eu-repo/grantAgreement/MCINN//FJCI-2017-32020
info:eu-repo/grantAgreement/MCINN//FJC2019-042422-I
http://dx.doi.org/10.3389/fvets.2021.744568
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosisArteche-Villasol, NoiveGutiérrez-Expósito, DanielElguezabal, N.Sevilla, I.A.Vallejo, R.Espinosa Cerrato, JoséFerreras, Mª del CarmenBenavides, JulioPérez Pérez, ValentínVaccinationParatuberculosisTuberculosisHIMBAdjuvantCross-protection16 páginas, 6 figuras, 3 tablas.Vaccination against paratuberculosis, a chronic disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map), has been considered as the most effective control method. However, protection is incomplete, and the mechanisms operating in the response of the animals to vaccination are not fully understood. Therefore, this study analyzed the immune response and the effects on protection against Map infection, elicited by paratuberculosis (Silirum®) and tuberculosis (heat-inactivated M. bovis [HIMB]) vaccines and their components in a caprine experimental model. Fifty goat kids were divided into 10 groups (n = 5) according to their vaccination (Silirum®, HIMB and nonvaccinated), immunization (inactivated bacteria or adjuvant), and/or infection. Oral challenge with Map was performed 45 days postvaccination/immunization (dpv), and animals were euthanized at 190 dpv. Peripheral immune response and proportion of lymphocyte subpopulations were assessed monthly by enzyme-linked immunosorbent assay and flow cytometry analysis, respectively. Local immune response, proportion of tissue lymphocyte subpopulations, Map detection (polymerase chain reaction), and histological examination were conducted in gut-associated lymphoid tissues. All infected groups developed paratuberculosis granulomatous lesions despite vaccination or immunization. The Silirum® and HIMB-vaccinated groups showed a considerable lesion reduction consistent with a significant peripheral cellular and humoral immune response. Besides, a lower number of granulomas were observed in groups immunized with inactivated bacteria and adjuvants in comparison to nonvaccinated and infected group. However, despite not being significant, this reduction was even higher in adjuvant immunized groups, which developed milder granulomatous lesion with no detectable peripheral immune responses associated with immunization. No changes in the peripheral and local proportion of lymphocyte subsets or local immune response were detected in relation to either vaccination/immunization or infection. Despite that paratuberculosis and tuberculosis vaccination showed a partial and cross-protection against Map infection, respectively, only histological examination could assess the progression of infection in these animals. In addition, the pattern observed in the reduction of the lesions in adjuvant immunized groups suggests the possible involvement of a nonspecific immune response that reduces the development of granulomatous lesions.This work was financially supported by the Spanish Ministry of Science and Innovation (projects AGL2015- 66540-C2-1-R and RTI2018-099496-B-I00), Junta de Castilla y León (project LE259P18), and National Institute for Agronomic Research (project RTA 2017- 00089-00-00). NA-V was the recipient of a predoctoral contract (BES-2016-076513) from the Spanish Ministry of Science and Innovation and DG-E and JE of a postdoctoral contract from the Ministry of Science and Innovation (grants nos. FJCI-2017-32020 and FJC2019-042422-I respectively).Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Junta de Castilla y LeónCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Arteche Villasol, Noive [0000-0001-5793-9578]Gutiérrez Expósito, Daniel [0000-0002-7683-623X]Espinosa Cerrato, José [0000-0002-9036-1402]Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]Benavides, Julio [0000-0001-9706-100X]Pérez Pérez, Valentín [0000-0003-0075-1587]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/271708reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-66540-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00info:eu-repo/grantAgreement/INIA//RTA 2017-00089-00-00info:eu-repo/grantAgreement/MCINN//FJCI-2017-32020info:eu-repo/grantAgreement/MCINN//FJC2019-042422-Ihttp://dx.doi.org/10.3389/fvets.2021.744568Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2717082026-05-22T06:33:51Z
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