Gold Nanocluster-Promoted Interfacial Electron Transfer of Cytochrome c at an Aqueous–Organic Interface
Functional enzyme–gold nanoparticle bioconjugates are becoming increasingly important in bioelectrocatalysis since they facilitate and improve the efficiency of long-range protein interfacial electron transfer between enzymes and electrodes by enhancing conductivity. While much research has focused...
| Authors: | , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2025 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/413613 |
| Online Access: | http://hdl.handle.net/10261/413613 https://www.scopus.com/inward/record.uri?eid=2-s2.0-105025597624&doi=10.1021%2Facs.nanolett.5c05030&partnerID=40&md5=7fccb94ecd074ab1c7e07c302d65b5c4 |
| Access Level: | Open access |
| Keyword: | bioconjugate bioelectrochemistry cytochrome c Gold nanocluster liquid−liquid electrochemistry |
| Summary: | Functional enzyme–gold nanoparticle bioconjugates are becoming increasingly important in bioelectrocatalysis since they facilitate and improve the efficiency of long-range protein interfacial electron transfer between enzymes and electrodes by enhancing conductivity. While much research has focused on solid–liquid interfaces, there is still limited understanding of the key parameters and electrochemical conditions necessary for reliable and reproducible bioelectrochemistry at polarizable aqueous–organic interfaces under native conditions. Herein, we demonstrate how the size of gold-modified nanoparticles influences the interfacial electron transfer of cytochrome c at an aqueous–organic interface. We found that nanoclusters centered in a size of 1.2 nm, equivalent to the water-trifluorotoluene mixed solvent layer (ca. 1.5 nm), work in tandem with cytochrome c to facilitate oxygen reduction reactions. In contrast, bioconjugates comprising larger gold nanoparticles are less effective in enhancing cytochrome c electrochemistry, with the gold nanoparticles acting as independent catalysts at the interface. © 2025 The Authors. Published by American Chemical Society |
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