Drug delivery system based on an antibacterial layer-by-layer coating on urinary catheters: an experimental and simulation approach

Urinary catheters (UCs) are critical in biomedical applications, but prolonged use increases the risk of catheter-associated urinary tract infections (CAUTIs), a leading cause of healthcare-associated infections (HAIs). The present study presents a dual strategy to create an antibacterial surface on...

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Detalhes bibliográficos
Autores: Pulido, Ruth, Naveas, Nelson, Fernández-Alonso, Francisco Javier, Manso Silván, Miguel, Soriano, Leonardo, Torres-Ulloa, Carlos, Mena-Ulecia, Karel, Recio-Sánchez, Gonzalo, Garcia-Sandoval, Juan Paulo, Hernández-Montelongo, Jacobo
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:dnet:biblosearchi::b70bec6bf22a82ffbcdd854493619bb3
Acesso em linha:https://hdl.handle.net/10486/767140
https://dx.doi.org/10.3389/fbioe.2025.1614509
Access Level:acceso abierto
Palavra-chave:Drug delivery
layer-by-layer (LbL)
antibacterial coating
urinary catheters
mathematical model
molecular dynamics
Física
Descrição
Resumo:Urinary catheters (UCs) are critical in biomedical applications, but prolonged use increases the risk of catheter-associated urinary tract infections (CAUTIs), a leading cause of healthcare-associated infections (HAIs). The present study presents a dual strategy to create an antibacterial surface on commercial Foley silicone UCs by combining a contact-killing effect with the controlled release of antimicrobial compounds. We designed a drug delivery system using a layer-by-layer (LbL) antibacterial coating of carboxymethylcellulose (CMC) and chitosan-silver (CHI-Ag) complexes, with ciprofloxacin (CFX) as the model drug. The resulting LbL coating, about 1 μm thick, incorporated Ag0 and demonstrated a high capacity for CFX loading, releasing over twice the amount (70 μg/cm2) compared to uncoated UCs (30 μg/cm2). The antibacterial efficacy was significantly higher in the LbL-coated samples, particularly against S. aureus compared to E. coli. Drug release experiments, modeled using Fick’s second law, indicated a diffusivity of 1.744 × 10−5 cm2/h. Our mathematical model predicts how variations in drug loading and rest times impact release profiles. Finally, molecular dynamics simulations suggested strong compatibility between CFX and the LbL layers, though with relatively low stability. This dual strategy holds promise for reducing CAUTIs effectively