Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms

10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019J

Detalles Bibliográficos
Autores: Medina-Pérez, Noemí Inmaculada, Santos, Francisco Javier, Berdalet, Elisa, Moyano, Encarnación
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330512
Acceso en línea:http://hdl.handle.net/10261/330512
Access Level:acceso abierto
Palabra clave:http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
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spelling Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata bloomsMedina-Pérez, Noemí InmaculadaSantos, Francisco JavierBerdalet, ElisaMoyano, Encarnaciónhttp://metadata.un.org/sdg/14Conserve and sustainably use the oceans, seas and marine resources for sustainable development10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019JAnalogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus Ostreopsis. The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced toxins can be transferred through marine food webs. Thus, the determination of the concentration of PLTX analogues (ovatoxins—OVTXs, ostreocins—OSTs and isobaric PLTX) in different matrices (seawater, marine fauna, etc.) is necessary to protect human health. This study is addressed to overcome some of the challenges that the chemical complexity of these molecules poses to their quantification by ultra-high-performance liquid chromatography high-resolution mass spectrometry-based techniques (UHPLC-HRMS). In particular, the mass spectra of the palytoxin analogues show the presence of a large number of ions (including mono- and multiply charged ions) whose nature, relative abundances and behavior can lead to quantitation errors if the correct ions are not selected. In this work, the variability of the PLTX and OVTX profiles under different instrument conditions, including the use of diverse electrospray generation sources and different quantitation methods, is studied. Moreover, the extraction protocol in seawater containing Ostreopsis sp. ovata cells is also evaluated. The use of a heated electrospray operating at 350 °C and a quantitative method including ions from different multiply charged species provides a more robust and reliable method for overcoming the problems due to the variability in the toxin's mass spectrum profile. A single MeOH : H2O (80 : 20, v/v) extraction is proposed as the best and reliable procedure. The overall method proposed was applied to quantify OVTXs (-a to -g) and iso-PLTX along the 2019 Ostreopsis cf. ovata bloom. The cells contained a total toxin concentration of up to 20.39 pg per cellThe authors thank the different financing agencies that supported the study. N. I. M.-P. was granted the fellowship FISDUR2020-00254 (AGAUR, Government of Catalonia). Financial support: MICIU (Spain, project PGC2018-095013-B-I00), AGAUR (Generalitat de Catalunya, project 2017SGR-310) and the Water Research Institute (IdRA, University of Barcelona). Support was provided also by the CoCliME project, an ERA4CS network (ERA-NET) initiated by JPI Climate and co-financed by FORMAS (SE) and the European Union (Grant no. 690462), ERC-2019-CoG-863934-GlycoSpec (European Research Council Consolidator Grant)With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewedRoyal Society of Chemistry (UK)Generalitat de CatalunyaMinisterio de Ciencia, Innovación y Universidades (España)European CommissionEuropean Research CouncilAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/330512reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095013-B-I00info:eu-repo/grantAgreement/EC/H2020/690462https://doi.org/10.1039/D2AY02019JSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3305122026-05-22T06:33:51Z
dc.title.none.fl_str_mv Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
spellingShingle Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
Medina-Pérez, Noemí Inmaculada
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
title_short Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_fullStr Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full_unstemmed Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_sort Multiply charged ion profiles in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
dc.creator.none.fl_str_mv Medina-Pérez, Noemí Inmaculada
Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
author Medina-Pérez, Noemí Inmaculada
author_facet Medina-Pérez, Noemí Inmaculada
Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
author_role author
author2 Santos, Francisco Javier
Berdalet, Elisa
Moyano, Encarnación
author2_role author
author
author
dc.contributor.none.fl_str_mv Generalitat de Catalunya
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
European Research Council
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
topic http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
description 10 pages, 5 figures, 1 table, supplementary information https://doi.org/10.1039/D2AY02019J
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330512
url http://hdl.handle.net/10261/330512
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095013-B-I00
info:eu-repo/grantAgreement/EC/H2020/690462
https://doi.org/10.1039/D2AY02019J
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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