Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability

Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates fro...

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Autores: González Díaz, Aida, Carrera Salinas, Anna, Pinto, Miguel, Cubero, Meritxell, Ende, Arie van der, Langereis, Jeroen D., Domínguez, M. Ángeles, Ardanuy, Carmen, Bajanca Lavado, Paula, Marti, Sara
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/184448
Acceso en línea:https://hdl.handle.net/2445/184448
Access Level:acceso abierto
Palabra clave:Bacils
Genòmica
Bacillus (Bacteria)
Genomics
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spelling Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stabilityGonzález Díaz, AidaCarrera Salinas, AnnaPinto, MiguelCubero, MeritxellEnde, Arie van derLangereis, Jeroen D.Domínguez, M. ÁngelesArdanuy, CarmenBajanca Lavado, PaulaMarti, SaraBacilsGenòmicaBacillus (Bacteria)GenomicsHaemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation.Springer Science and Business Media LLC2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/184448Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-022-07185-5Scientific Reports, 2022, vol 12, num 1https://doi.org/10.1038/s41598-022-07185-5cc by (c) Gonzalez Diaz, Aida et al, 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1844482026-05-29T05:05:01Z
dc.title.none.fl_str_mv Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
title Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
spellingShingle Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
González Díaz, Aida
Bacils
Genòmica
Bacillus (Bacteria)
Genomics
title_short Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
title_full Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
title_fullStr Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
title_full_unstemmed Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
title_sort Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
dc.creator.none.fl_str_mv González Díaz, Aida
Carrera Salinas, Anna
Pinto, Miguel
Cubero, Meritxell
Ende, Arie van der
Langereis, Jeroen D.
Domínguez, M. Ángeles
Ardanuy, Carmen
Bajanca Lavado, Paula
Marti, Sara
author González Díaz, Aida
author_facet González Díaz, Aida
Carrera Salinas, Anna
Pinto, Miguel
Cubero, Meritxell
Ende, Arie van der
Langereis, Jeroen D.
Domínguez, M. Ángeles
Ardanuy, Carmen
Bajanca Lavado, Paula
Marti, Sara
author_role author
author2 Carrera Salinas, Anna
Pinto, Miguel
Cubero, Meritxell
Ende, Arie van der
Langereis, Jeroen D.
Domínguez, M. Ángeles
Ardanuy, Carmen
Bajanca Lavado, Paula
Marti, Sara
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacils
Genòmica
Bacillus (Bacteria)
Genomics
topic Bacils
Genòmica
Bacillus (Bacteria)
Genomics
description Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184448
url https://hdl.handle.net/2445/184448
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-07185-5
Scientific Reports, 2022, vol 12, num 1
https://doi.org/10.1038/s41598-022-07185-5
dc.rights.none.fl_str_mv cc by (c) Gonzalez Diaz, Aida et al, 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Gonzalez Diaz, Aida et al, 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Springer Science and Business Media LLC
publisher.none.fl_str_mv Springer Science and Business Media LLC
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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