The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons

Producción Científica

Detalhes bibliográficos
Autores: Núñez Llorente, Lucía, Villalobos Jorge, Carlos, Boockfor, Fredric R., Frawley, L. Stephen
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2000
País:España
Recursos:Universidad de Valladolid
Repositório:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/45076
Acesso em linha:https://doi.org/10.1210/endo.141.6.7491
http://uvadoc.uva.es/handle/10324/45076
Access Level:Acceso aberto
Palavra-chave:Neuroendocrinology
Neuroendocrinología
id ES_9b00ffe6d052d1eb6c153d1b6bfccc0c
oai_identifier_str oai:uvadoc.uva.es:10324/45076
network_acronym_str ES
network_name_str España
repository_id_str
spelling The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neuronsNúñez Llorente, LucíaVillalobos Jorge, CarlosBoockfor, Fredric R.Frawley, L. StephenNeuroendocrinologyNeuroendocrinologíaProducción CientíficaIt is well established that pulsatile release of GnRH regulates the reproductive axis, but little is known about the mechanisms underlying this pulsatility. Recent findings that GT1 cells, a line derived from the mouse embryonic hypothalamus, release GnRH in a pulsatile manner indicates that this rhythmic activity is an intrinsic property of GnRH neurons. In several attempts to uncover the intracellular basis for this pulsatile phenomenon, it was revealed that intracellular calcium concentrations change in a rhythmic fashion in GnRH neurons and that cellular depolarization, which triggers a secretory event, is associated with profound calcium changes in the cells. These findings raised the intriguing possibility that periodic alterations in intracellular calcium concentrations may underlie the phenomenon of pulsatile secretion in GnRH neurons. To address this, we first adapted the use of FM1–43 fluorescence to monitor changes of secretion in individual GT1–7 cells and then combined this approach with simultaneous measurement of intracellular free calcium ([Ca2+]i, fura 2 method). In initial validation experiments, we found that stimulation of exocytosis with K+ (75 mM) or N-methyl-D-aspartate (NMDA, 100μ M) predictably evoked dynamic increases of both FM1–43 and fura 2 fluorescence. Later measurement of calcium dynamics and exocytotic activity in unstimulated cells revealed that[ Ca2+]i underwent transitions from quiescence to high oscillatory behavior, and that these shifts were frequently associated with exocytotic events. Moreover, these calcium oscillatory transitions and associated changes in secretory activity occurred synchronously among most adjacent cells and at a frequency similar to that reported for pulsatile release of GnRH by entire cultures of GnRH neurons. Taken together, these results indicate that the intrinsic secretory pulsatility of GnRH neurons appears to be a consequence of coordinated, periodic changes in the pattern of calcium oscillations within individual cells.National Institutes of Health (grants DK-38215 and HD-37657)Oxford University Press2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1210/endo.141.6.7491http://uvadoc.uva.es/handle/10324/45076reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://academic.oup.com/endo/article/141/6/2012/2988424info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/oai:uvadoc.uva.es:10324/450762026-06-13T12:44:47Z
dc.title.none.fl_str_mv The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
title The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
spellingShingle The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
Núñez Llorente, Lucía
Neuroendocrinology
Neuroendocrinología
title_short The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
title_full The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
title_fullStr The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
title_full_unstemmed The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
title_sort The relationship between pulsatile secretion and calcium dynamics in single, living gonadotropin-releasing hormone neurons
dc.creator.none.fl_str_mv Núñez Llorente, Lucía
Villalobos Jorge, Carlos
Boockfor, Fredric R.
Frawley, L. Stephen
author Núñez Llorente, Lucía
author_facet Núñez Llorente, Lucía
Villalobos Jorge, Carlos
Boockfor, Fredric R.
Frawley, L. Stephen
author_role author
author2 Villalobos Jorge, Carlos
Boockfor, Fredric R.
Frawley, L. Stephen
author2_role author
author
author
dc.subject.none.fl_str_mv Neuroendocrinology
Neuroendocrinología
topic Neuroendocrinology
Neuroendocrinología
description Producción Científica
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1210/endo.141.6.7491
http://uvadoc.uva.es/handle/10324/45076
url https://doi.org/10.1210/endo.141.6.7491
http://uvadoc.uva.es/handle/10324/45076
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://academic.oup.com/endo/article/141/6/2012/2988424
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414468254957568
score 15,301629

Registros relacionados