Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models
Mammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent chromatin remodelers whose subunits have emerged among the most frequently mutated genes in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities in SWI/SNF-mutant tumors that can lead...
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/65775 |
| Acesso em linha: | https://hdl.handle.net/10171/65775 |
| Access Level: | acceso abierto |
| Palavra-chave: | SWI/SNF complex Lung cancer Lung adenocarcinoma Epigenetics Cell models Multi-omics |
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Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell ModelsPeinado, P. (Paola)|||/items/1dc3ecb0-434d-4544-83e4-211962d2f4adAndrades, A. (Alvaro)|||/items/e2b658b8-c400-4cab-9860-914b423a5d54Cuadros, M. (Marta)|||/items/e8264bf7-3f94-4449-ad23-7baaba4fa9bcRodriguez-Lara, M.I. (Maria Isabel)|||/items/d4a12388-2ca2-473f-bc98-4c95baebea6dCoira, I.F. (Isabel F.)|||/items/1948f876-fb8d-446f-a52a-36f7aab16d66Garcia, D.J. (Daniel J.)|||/items/d73b79b5-31c7-4b3c-9176-d7fd81cd747dÁlvarez-Perez, J.C. (Juan Carlos)|||/items/03360ef7-e6fe-4791-b844-ddf2481b75eeBaliñas-Gavira, C. (Carlos)|||/items/e32bc8ba-c624-468e-bc51-88dd94357fdcArenas, A.M. (Alberto M.)|||/items/4793dbe3-ff0a-4d0a-ba68-8414fdc6ce48Patiño-Mercau, J.R. (Juan Rodrigo)|||/items/88987f7d-ffb1-4841-a829-3177239e1190Sanjuan-Hidalgo, J. (Juan)|||/items/a8165a36-8176-4a2b-a863-f690e6ffd3a6Romero, O.A. (Octavio A.)|||/items/946c1a42-0510-415f-9b6e-4ce94b463730Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596Carretero, J. (Julian)|||/items/c809e00c-b070-41aa-98a2-dfe39b3ff0f3Sanchez-Cespedes, M. (Montserrat)|||/items/aaf33cf5-6f66-47a3-9c7b-f14e57d3cc88Medina, P.P. (Pedro P.)|||/items/49f11e06-d63d-46b1-a642-bcff0f620dbdSWI/SNF complexLung cancerLung adenocarcinomaEpigeneticsCell modelsMulti-omicsMammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent chromatin remodelers whose subunits have emerged among the most frequently mutated genes in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities in SWI/SNF-mutant tumors that can lead to the discovery of new therapeutic drugs. However, choosing an appropriate cancer cell line model for SWI/SNF functional studies can be challenging because SWI/SNF subunits are frequently altered in cancer by various mechanisms, including genetic alterations and post-transcriptional mechanisms. In this work, we combined genomic, transcriptomic, and proteomic approaches to study the mutational status and the expression levels of the SWI/SNF subunits in a panel of 38 lung adenocarcinoma (LUAD) cell lines. We found that the SWI/SNF complex was mutated in more than 76% of our LUAD cell lines and there was a high variability in the expression of the different SWI/SNF subunits. These results underline the importance of the SWI/SNF complex as a tumor suppressor in LUAD and the difficulties in defining altered and unaltered cell models for the SWI/SNF complex. These findings will assist researchers in choosing the most suitable cellular models for their studies of SWI/SNF to bring all of its potential to the development of novel therapeutic applications.Dadun. Depósito Académico Digital Universidad de Navarra20232023-03-2320202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/65775reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/657752026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| title |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| spellingShingle |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models Peinado, P. (Paola)|||/items/1dc3ecb0-434d-4544-83e4-211962d2f4ad SWI/SNF complex Lung cancer Lung adenocarcinoma Epigenetics Cell models Multi-omics |
| title_short |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| title_full |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| title_fullStr |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| title_full_unstemmed |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| title_sort |
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models |
| dc.creator.none.fl_str_mv |
Peinado, P. (Paola)|||/items/1dc3ecb0-434d-4544-83e4-211962d2f4ad Andrades, A. (Alvaro)|||/items/e2b658b8-c400-4cab-9860-914b423a5d54 Cuadros, M. (Marta)|||/items/e8264bf7-3f94-4449-ad23-7baaba4fa9bc Rodriguez-Lara, M.I. (Maria Isabel)|||/items/d4a12388-2ca2-473f-bc98-4c95baebea6d Coira, I.F. (Isabel F.)|||/items/1948f876-fb8d-446f-a52a-36f7aab16d66 Garcia, D.J. (Daniel J.)|||/items/d73b79b5-31c7-4b3c-9176-d7fd81cd747d Álvarez-Perez, J.C. (Juan Carlos)|||/items/03360ef7-e6fe-4791-b844-ddf2481b75ee Baliñas-Gavira, C. (Carlos)|||/items/e32bc8ba-c624-468e-bc51-88dd94357fdc Arenas, A.M. (Alberto M.)|||/items/4793dbe3-ff0a-4d0a-ba68-8414fdc6ce48 Patiño-Mercau, J.R. (Juan Rodrigo)|||/items/88987f7d-ffb1-4841-a829-3177239e1190 Sanjuan-Hidalgo, J. (Juan)|||/items/a8165a36-8176-4a2b-a863-f690e6ffd3a6 Romero, O.A. (Octavio A.)|||/items/946c1a42-0510-415f-9b6e-4ce94b463730 Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Carretero, J. (Julian)|||/items/c809e00c-b070-41aa-98a2-dfe39b3ff0f3 Sanchez-Cespedes, M. (Montserrat)|||/items/aaf33cf5-6f66-47a3-9c7b-f14e57d3cc88 Medina, P.P. (Pedro P.)|||/items/49f11e06-d63d-46b1-a642-bcff0f620dbd |
| author |
Peinado, P. (Paola)|||/items/1dc3ecb0-434d-4544-83e4-211962d2f4ad |
| author_facet |
Peinado, P. (Paola)|||/items/1dc3ecb0-434d-4544-83e4-211962d2f4ad Andrades, A. (Alvaro)|||/items/e2b658b8-c400-4cab-9860-914b423a5d54 Cuadros, M. (Marta)|||/items/e8264bf7-3f94-4449-ad23-7baaba4fa9bc Rodriguez-Lara, M.I. (Maria Isabel)|||/items/d4a12388-2ca2-473f-bc98-4c95baebea6d Coira, I.F. (Isabel F.)|||/items/1948f876-fb8d-446f-a52a-36f7aab16d66 Garcia, D.J. (Daniel J.)|||/items/d73b79b5-31c7-4b3c-9176-d7fd81cd747d Álvarez-Perez, J.C. (Juan Carlos)|||/items/03360ef7-e6fe-4791-b844-ddf2481b75ee Baliñas-Gavira, C. (Carlos)|||/items/e32bc8ba-c624-468e-bc51-88dd94357fdc Arenas, A.M. (Alberto M.)|||/items/4793dbe3-ff0a-4d0a-ba68-8414fdc6ce48 Patiño-Mercau, J.R. (Juan Rodrigo)|||/items/88987f7d-ffb1-4841-a829-3177239e1190 Sanjuan-Hidalgo, J. (Juan)|||/items/a8165a36-8176-4a2b-a863-f690e6ffd3a6 Romero, O.A. (Octavio A.)|||/items/946c1a42-0510-415f-9b6e-4ce94b463730 Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Carretero, J. (Julian)|||/items/c809e00c-b070-41aa-98a2-dfe39b3ff0f3 Sanchez-Cespedes, M. (Montserrat)|||/items/aaf33cf5-6f66-47a3-9c7b-f14e57d3cc88 Medina, P.P. (Pedro P.)|||/items/49f11e06-d63d-46b1-a642-bcff0f620dbd |
| author_role |
author |
| author2 |
Andrades, A. (Alvaro)|||/items/e2b658b8-c400-4cab-9860-914b423a5d54 Cuadros, M. (Marta)|||/items/e8264bf7-3f94-4449-ad23-7baaba4fa9bc Rodriguez-Lara, M.I. (Maria Isabel)|||/items/d4a12388-2ca2-473f-bc98-4c95baebea6d Coira, I.F. (Isabel F.)|||/items/1948f876-fb8d-446f-a52a-36f7aab16d66 Garcia, D.J. (Daniel J.)|||/items/d73b79b5-31c7-4b3c-9176-d7fd81cd747d Álvarez-Perez, J.C. (Juan Carlos)|||/items/03360ef7-e6fe-4791-b844-ddf2481b75ee Baliñas-Gavira, C. (Carlos)|||/items/e32bc8ba-c624-468e-bc51-88dd94357fdc Arenas, A.M. (Alberto M.)|||/items/4793dbe3-ff0a-4d0a-ba68-8414fdc6ce48 Patiño-Mercau, J.R. (Juan Rodrigo)|||/items/88987f7d-ffb1-4841-a829-3177239e1190 Sanjuan-Hidalgo, J. (Juan)|||/items/a8165a36-8176-4a2b-a863-f690e6ffd3a6 Romero, O.A. (Octavio A.)|||/items/946c1a42-0510-415f-9b6e-4ce94b463730 Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Carretero, J. (Julian)|||/items/c809e00c-b070-41aa-98a2-dfe39b3ff0f3 Sanchez-Cespedes, M. (Montserrat)|||/items/aaf33cf5-6f66-47a3-9c7b-f14e57d3cc88 Medina, P.P. (Pedro P.)|||/items/49f11e06-d63d-46b1-a642-bcff0f620dbd |
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author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
SWI/SNF complex Lung cancer Lung adenocarcinoma Epigenetics Cell models Multi-omics |
| topic |
SWI/SNF complex Lung cancer Lung adenocarcinoma Epigenetics Cell models Multi-omics |
| description |
Mammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent chromatin remodelers whose subunits have emerged among the most frequently mutated genes in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities in SWI/SNF-mutant tumors that can lead to the discovery of new therapeutic drugs. However, choosing an appropriate cancer cell line model for SWI/SNF functional studies can be challenging because SWI/SNF subunits are frequently altered in cancer by various mechanisms, including genetic alterations and post-transcriptional mechanisms. In this work, we combined genomic, transcriptomic, and proteomic approaches to study the mutational status and the expression levels of the SWI/SNF subunits in a panel of 38 lung adenocarcinoma (LUAD) cell lines. We found that the SWI/SNF complex was mutated in more than 76% of our LUAD cell lines and there was a high variability in the expression of the different SWI/SNF subunits. These results underline the importance of the SWI/SNF complex as a tumor suppressor in LUAD and the difficulties in defining altered and unaltered cell models for the SWI/SNF complex. These findings will assist researchers in choosing the most suitable cellular models for their studies of SWI/SNF to bring all of its potential to the development of novel therapeutic applications. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01 2020 2020-01-01 2023 2023-03-23 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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article |
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https://hdl.handle.net/10171/65775 |
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https://hdl.handle.net/10171/65775 |
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Inglés eng |
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Inglés |
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eng |
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open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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