Simvastatin and metformin inhibit cell growth in hepatitis C virus infected cells via mTOR increasing PTEN and autophagy

Hepatitis C virus (HCV) infection has been related to increased risk of development of hepa tocellular carcinoma (HCC) while metformin (M) and statins treatment seemed to protect against HCC development. In this work, we aim to identify the mechanisms by which metfor min and simvastatin (S) could pr...

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Detalles Bibliográficos
Autores: Campo, José Antonio del, García Valdecasas, Marta, Gil Gómez, Antonio, Rojas, Ángela, Gallego, Paloma, Ampuero Herrojo, Javier, Gallego Durán, Rocío, Pastor, Helena, Grande, Lourdes, Padillo Ruiz, Francisco Javier, Muntané Relat, Jordi, Romero Gómez, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/107436
Acceso en línea:https://hdl.handle.net/11441/107436
https://doi.org/10.1371/journal.pone.0191805
Access Level:acceso abierto
Palabra clave:Hepatitis C virus
HCV
Simvastatin
Metformin
mTOR
PTEN
Descripción
Sumario:Hepatitis C virus (HCV) infection has been related to increased risk of development of hepa tocellular carcinoma (HCC) while metformin (M) and statins treatment seemed to protect against HCC development. In this work, we aim to identify the mechanisms by which metfor min and simvastatin (S) could protect from liver cancer. Huh7.5 cells were infected with HCV particles and treated with M+S. Human primary hepatocytes were treated with M+S. Treatment with both drugs inhibited Huh7.5 cell growth and HCV infection. In non-infected cells S increased translational controlled tumor protein (TCTP) and phosphatase and tensin homolog (PTEN) proteins while M inhibited mammalian target of rapamycin (mTOR) and TCTP. Simvastatin and metformin co-administered down-regulated mTOR and TCTP, while PTEN was increased. In cells infected by HCV, mTOR, TCTP, p62 and light chain 3B II (LC3BII) were increased and PTEN was decreased. S+M treatment increased PTEN, p62 and LC3BII in Huh7.5 cells. In human primary hepatocytes, metformin treatment inhibited mTOR and PTEN, but up-regulated p62, LC3BII and Caspase 3. In conclusion, simvastatin and metformin inhibited cell growth and HCV infection in vitro. In human hepatocytes, met formin increased cell-death markers. These findings suggest that M+S treatment could be useful in therapeutic prevention of HCV-related hepatocellular carcinoma.

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