First report of glyphosate resistance in an Amaranthus palmeri population from Europe

The invasive weed Amaranthus palmeri is spreading throughout Spain, with Catalonia being one of the most affected regions. For this species, acetolactate synthase-inhibiting herbicide-resistant populations have been reported, and now glyphosate resistance is also suspected. Glyphosate targets and in...

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Autores: Manicardi, Alfredo, Milani, Andrea, Scarabel, Laura, Mora, Germán, Recasens i Guinjuan, Jordi, Llenes, Josep María, Montull, José María, Torra Farré, Joel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/463322
Acceso en línea:https://doi.org/10.1111/wre.12579
https://hdl.handle.net/10459.1/463322
Access Level:acceso abierto
Palabra clave:EPSPS inhibitor
EPSPS overexpression
Palmer amaranth
Roadside
Spain
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spelling First report of glyphosate resistance in an Amaranthus palmeri population from EuropeManicardi, AlfredoMilani, AndreaScarabel, LauraMora, GermánRecasens i Guinjuan, JordiLlenes, Josep MaríaMontull, José María Torra Farré, Joel EPSPS inhibitorEPSPS overexpressionPalmer amaranthRoadsideSpainThe invasive weed Amaranthus palmeri is spreading throughout Spain, with Catalonia being one of the most affected regions. For this species, acetolactate synthase-inhibiting herbicide-resistant populations have been reported, and now glyphosate resistance is also suspected. Glyphosate targets and inhibits the enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS), but A. palmeri has evolved different resistant mechanisms leading to plant survival. One of the most effective is the EPSPS overexpression due to copy number variation (CNV). Gene copies accumulate within the EPSPS cassette that is an extrachromosomal circular DNA displaying unique structural polymorphisms. This study aims to determine the response to glyphosate of a suspected resistant population collected from a roadside and investigate the resistance mechanism involved. The herbicide bioassay confirmed that 40% of the plants survived glyphosate applied at 540 g a.i. ha 1 . No known mutations endowing glyphosate resistance were found at EPSPS amongst confirmed resistant plants, while in most of them (70%) specific molecular markers revealed the presence of the EPSPS cassette. All these results indicate that this population is glyphosate resistant and it is very likely that the EPSPS gene CNV is the main resistance mechanism. This is the first case of glyphosate resistance in A. palmeri in Europe whose introduction is likely due to importation of contaminated seed with glyphosate-resistant Palmer amaranth from the Americas. This introduction poses a significant danger to summer crops in our continent.This work has been supported by the Spanish State Research Agency,Spain (AEI) and the European Regional Development Fund, EU (ERDF)through the projects AGL2017-83325-C4-2-R and PID2020-113229RB-C42. 2021-2024. Joel Torra acknowledges support from the SpanishMinistry of Science, Innovation, and Universities (grant Ramon y CajalRYC2018-023866-I). This project has received funding from the European Union's H2020 research and innovation program under MarieSklodowska-Curie grant agreement No 801586. The authors are gratefulto Alison Garside for revising the English text. The authors also thank twoanonymous reviewers for their helpful comments on the draft of themanuscript.WILEY2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1111/wre.12579https://hdl.handle.net/10459.1/463322reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42Reproducció del document publicat a https://doi.org/10.1111/wre.12579Weed Research, 2025, vol. 65, num. 1, e12579info:eu-repo/grantAgreement/EC/H2020/801586cc-by-nc-nd (c) Manicardi et al., 2023Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:repositori.udl.cat:10459.1/4633222026-06-24T12:42:17Z
dc.title.none.fl_str_mv First report of glyphosate resistance in an Amaranthus palmeri population from Europe
title First report of glyphosate resistance in an Amaranthus palmeri population from Europe
spellingShingle First report of glyphosate resistance in an Amaranthus palmeri population from Europe
Manicardi, Alfredo
EPSPS inhibitor
EPSPS overexpression
Palmer amaranth
Roadside
Spain
title_short First report of glyphosate resistance in an Amaranthus palmeri population from Europe
title_full First report of glyphosate resistance in an Amaranthus palmeri population from Europe
title_fullStr First report of glyphosate resistance in an Amaranthus palmeri population from Europe
title_full_unstemmed First report of glyphosate resistance in an Amaranthus palmeri population from Europe
title_sort First report of glyphosate resistance in an Amaranthus palmeri population from Europe
dc.creator.none.fl_str_mv Manicardi, Alfredo
Milani, Andrea
Scarabel, Laura
Mora, Germán
Recasens i Guinjuan, Jordi
Llenes, Josep María
Montull, José María
Torra Farré, Joel
author Manicardi, Alfredo
author_facet Manicardi, Alfredo
Milani, Andrea
Scarabel, Laura
Mora, Germán
Recasens i Guinjuan, Jordi
Llenes, Josep María
Montull, José María
Torra Farré, Joel
author_role author
author2 Milani, Andrea
Scarabel, Laura
Mora, Germán
Recasens i Guinjuan, Jordi
Llenes, Josep María
Montull, José María
Torra Farré, Joel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EPSPS inhibitor
EPSPS overexpression
Palmer amaranth
Roadside
Spain
topic EPSPS inhibitor
EPSPS overexpression
Palmer amaranth
Roadside
Spain
description The invasive weed Amaranthus palmeri is spreading throughout Spain, with Catalonia being one of the most affected regions. For this species, acetolactate synthase-inhibiting herbicide-resistant populations have been reported, and now glyphosate resistance is also suspected. Glyphosate targets and inhibits the enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS), but A. palmeri has evolved different resistant mechanisms leading to plant survival. One of the most effective is the EPSPS overexpression due to copy number variation (CNV). Gene copies accumulate within the EPSPS cassette that is an extrachromosomal circular DNA displaying unique structural polymorphisms. This study aims to determine the response to glyphosate of a suspected resistant population collected from a roadside and investigate the resistance mechanism involved. The herbicide bioassay confirmed that 40% of the plants survived glyphosate applied at 540 g a.i. ha 1 . No known mutations endowing glyphosate resistance were found at EPSPS amongst confirmed resistant plants, while in most of them (70%) specific molecular markers revealed the presence of the EPSPS cassette. All these results indicate that this population is glyphosate resistant and it is very likely that the EPSPS gene CNV is the main resistance mechanism. This is the first case of glyphosate resistance in A. palmeri in Europe whose introduction is likely due to importation of contaminated seed with glyphosate-resistant Palmer amaranth from the Americas. This introduction poses a significant danger to summer crops in our continent.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1111/wre.12579
https://hdl.handle.net/10459.1/463322
url https://doi.org/10.1111/wre.12579
https://hdl.handle.net/10459.1/463322
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42
Reproducció del document publicat a https://doi.org/10.1111/wre.12579
Weed Research, 2025, vol. 65, num. 1, e12579
info:eu-repo/grantAgreement/EC/H2020/801586
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Manicardi et al., 2023
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd (c) Manicardi et al., 2023
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
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