Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS

Adrenomedullin (ADM) is upregulated independently by hypoxia and LPS, two key factors in the pathogenesis of acute lung injury (ALI). This study evaluates the expression of ADM in ALI using experimental models combining both stimuli: an in vivo model of rats treated with LPS and acute normobaric hyp...

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Autores: Agorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7, Zulueta, J. (Javier)|||/items/1979cb86-b86c-41b7-8494-1f00acb7eb1f, Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596, Garayoa, M. (Mercedes)|||/items/a54f545f-343f-4c7d-98b6-4b7986d3ff2c
Tipo de recurso: artículo
Fecha de publicación:2005
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/16769
Acceso en línea:https://hdl.handle.net/10171/16769
Access Level:acceso abierto
Palabra clave:Acute respiratory distress syndrome
Endotoxin
Alveolar macrophages
Endothelial cells
Type II pneumocytes
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spelling Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPSAgorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7Zulueta, J. (Javier)|||/items/1979cb86-b86c-41b7-8494-1f00acb7eb1fMontuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596Garayoa, M. (Mercedes)|||/items/a54f545f-343f-4c7d-98b6-4b7986d3ff2cAcute respiratory distress syndromeEndotoxinAlveolar macrophagesEndothelial cellsType II pneumocytesAdrenomedullin (ADM) is upregulated independently by hypoxia and LPS, two key factors in the pathogenesis of acute lung injury (ALI). This study evaluates the expression of ADM in ALI using experimental models combining both stimuli: an in vivo model of rats treated with LPS and acute normobaric hypoxia (9% O2) and an in vitro model of rat lung cell lines cultured with LPS and exposed to hypoxia (1% O2). ADM expression was analyzed by in situ hybridization, Northern blot, Western blot, and RIA analyses. In the rat lung, combination of hypoxia and LPS treatments overcomes ADM induction occurring after each treatment alone. With in situ techniques, the synergistic effect of both stimuli mainly correlates with ADM expression in inflammatory cells within blood vessels and, to a lesser extent, to cells in the lung parenchyma and bronchiolar epithelial cells. In the in vitro model, hypoxia and hypoxia LPS treatments caused a similar strong induction of ADM expression and secretion in epithelial and endothelial cell lines. In alveolar macrophages, however, LPS-induced ADM expression and secretion were further increased by the concomitant exposure to hypoxia, thus paralleling the in vivo response. In conclusion, ADM expression is highly induced in a variety of key lung cell types in this rat model of ALI by combination of hypoxia and LPS, suggesting an essential role for this mediator in this syndromeAmerican Physiological SocietyDadun. Depósito Académico Digital Universidad de Navarra20112011-03-0120052005-01-0120052005-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/16769reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/167692026-06-21T12:47:57Z
dc.title.none.fl_str_mv Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
title Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
spellingShingle Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
Agorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7
Acute respiratory distress syndrome
Endotoxin
Alveolar macrophages
Endothelial cells
Type II pneumocytes
title_short Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
title_full Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
title_fullStr Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
title_full_unstemmed Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
title_sort Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
dc.creator.none.fl_str_mv Agorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7
Zulueta, J. (Javier)|||/items/1979cb86-b86c-41b7-8494-1f00acb7eb1f
Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596
Garayoa, M. (Mercedes)|||/items/a54f545f-343f-4c7d-98b6-4b7986d3ff2c
author Agorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7
author_facet Agorreta, J. (Jackeline)|||/items/885eb615-5802-4d49-9542-91de9bb95ff7
Zulueta, J. (Javier)|||/items/1979cb86-b86c-41b7-8494-1f00acb7eb1f
Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596
Garayoa, M. (Mercedes)|||/items/a54f545f-343f-4c7d-98b6-4b7986d3ff2c
author_role author
author2 Zulueta, J. (Javier)|||/items/1979cb86-b86c-41b7-8494-1f00acb7eb1f
Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596
Garayoa, M. (Mercedes)|||/items/a54f545f-343f-4c7d-98b6-4b7986d3ff2c
author2_role author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Acute respiratory distress syndrome
Endotoxin
Alveolar macrophages
Endothelial cells
Type II pneumocytes
topic Acute respiratory distress syndrome
Endotoxin
Alveolar macrophages
Endothelial cells
Type II pneumocytes
description Adrenomedullin (ADM) is upregulated independently by hypoxia and LPS, two key factors in the pathogenesis of acute lung injury (ALI). This study evaluates the expression of ADM in ALI using experimental models combining both stimuli: an in vivo model of rats treated with LPS and acute normobaric hypoxia (9% O2) and an in vitro model of rat lung cell lines cultured with LPS and exposed to hypoxia (1% O2). ADM expression was analyzed by in situ hybridization, Northern blot, Western blot, and RIA analyses. In the rat lung, combination of hypoxia and LPS treatments overcomes ADM induction occurring after each treatment alone. With in situ techniques, the synergistic effect of both stimuli mainly correlates with ADM expression in inflammatory cells within blood vessels and, to a lesser extent, to cells in the lung parenchyma and bronchiolar epithelial cells. In the in vitro model, hypoxia and hypoxia LPS treatments caused a similar strong induction of ADM expression and secretion in epithelial and endothelial cell lines. In alveolar macrophages, however, LPS-induced ADM expression and secretion were further increased by the concomitant exposure to hypoxia, thus paralleling the in vivo response. In conclusion, ADM expression is highly induced in a variety of key lung cell types in this rat model of ALI by combination of hypoxia and LPS, suggesting an essential role for this mediator in this syndrome
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01
2005
2005-01-01
2011
2011-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/16769
url https://hdl.handle.net/10171/16769
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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