Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome

Diplodia sapinea (Fr.) Fuckelis is an opportunistic pathogen of Scots pine (Pinus sylvestris (L.) that causes Diplodia tip blight following host stress. The factors driving its shift from endophyte to pathogen are not well understood, particularly in relation to the surrounding fungal community. The...

Descripción completa

Detalles Bibliográficos
Autores: Brodde, Laura, Miñana-Posada, Silvia, Tudoran, Amelia, Angel Redondo, Miguel, Elfstrand, Malin, Oliva Palau, Jonàs, Stenlid, Jan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/468190
Acceso en línea:https://doi.org/10.1016/j.foreco.2025.122781
https://hdl.handle.net/10459.1/468190
Access Level:acceso abierto
Palabra clave:Crown dieback
Endophytic fungal community
Metabarcoding
id ES_99efe79ef67d3b5f36afe0ec61ca4e32
oai_identifier_str oai:repositori.udl.cat:10459.1/468190
network_acronym_str ES
network_name_str España
repository_id_str
spelling Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiomeBrodde, LauraMiñana-Posada, SilviaTudoran, AmeliaAngel Redondo, MiguelElfstrand, MalinOliva Palau, JonàsStenlid, JanCrown diebackEndophytic fungal communityMetabarcodingDiplodia sapinea (Fr.) Fuckelis is an opportunistic pathogen of Scots pine (Pinus sylvestris (L.) that causes Diplodia tip blight following host stress. The factors driving its shift from endophyte to pathogen are not well understood, particularly in relation to the surrounding fungal community. The objective of the current study was to determine the association of D. sapinea and the composition of the general endophyte community with symptomatic and asymptomatic sites, trees, twigs and tissues in an ongoing outbreak of Diplodia tip blight. The endophytic fungal community was characterized using metabarcoding of the ribosomal ITS2 region. We found that variation in fungal community composition was most influenced by differences between sites, highlighting the importance of site-specific environmental conditions such as previous drought impact and associated crown dieback. However, the fungal communities also varied between symptomatic and asymptomatic trees indicating the significance of tree health. The fungal communities of symptomatic trees, especially in twigs with tip blight symptoms, included D. sapinea, Therrya pini, and Lophodermium arboricola. These results are consistent with the balanced antagonism hypothesis, suggesting that shifts in community composition under stress may facilitate the transition of D. sapinea from a latent endophyte to a pathogen. D. sapinea was found in both healthy and symptomatic twigs, with a ∼60-fold increase in symptomatic tissues. Site differences accounted for 42.6 % of fungal community variation. In contrast, taxa from the order Phaothecales were more abundant in asymptomatic twigs and in healthy tissues of of symptomatic twigs - suggesting potential antagonism. Our findings provide insights into early disease detection and underscore the importance of monitoring endophyte community shifts to support Scots pine forest resilience under climate stress.The authors are grateful for field assistance from Maria Jonsson and Hern\u00E1n Dario Capador-Barreto, logistic support from Karin W\u00E5gstr\u00F6m (Swedish Forest Agency) and SciLifeLab for the sequencing. Funding was provided by a grant from The Swedish research council FORMAS (grant number 2017\u201300626 ) to JS and T4F to ME. JO contribution was partly funded by Ministry of Science, Innovation and Universities MICIU of Spain (grant number PID2021\u2013127328OB-I00).Elsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.foreco.2025.122781https://hdl.handle.net/10459.1/468190reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI//PID2021-127328OB-I00Reproducció del document publicat a https://doi.org/10.1016/j.foreco.2025.122781Forest Ecology and Management, 2025, vol. 589, núm. 122781, p. 1-12cc-by (c) Brodde et al., 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4681902026-06-24T12:42:17Z
dc.title.none.fl_str_mv Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
title Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
spellingShingle Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
Brodde, Laura
Crown dieback
Endophytic fungal community
Metabarcoding
title_short Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
title_full Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
title_fullStr Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
title_full_unstemmed Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
title_sort Diplodia tip blight (Diplodia sapinea) and site conditions shape Scots pine (Pinus sylvestris) endophytic mycobiome
dc.creator.none.fl_str_mv Brodde, Laura
Miñana-Posada, Silvia
Tudoran, Amelia
Angel Redondo, Miguel
Elfstrand, Malin
Oliva Palau, Jonàs
Stenlid, Jan
author Brodde, Laura
author_facet Brodde, Laura
Miñana-Posada, Silvia
Tudoran, Amelia
Angel Redondo, Miguel
Elfstrand, Malin
Oliva Palau, Jonàs
Stenlid, Jan
author_role author
author2 Miñana-Posada, Silvia
Tudoran, Amelia
Angel Redondo, Miguel
Elfstrand, Malin
Oliva Palau, Jonàs
Stenlid, Jan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Crown dieback
Endophytic fungal community
Metabarcoding
topic Crown dieback
Endophytic fungal community
Metabarcoding
description Diplodia sapinea (Fr.) Fuckelis is an opportunistic pathogen of Scots pine (Pinus sylvestris (L.) that causes Diplodia tip blight following host stress. The factors driving its shift from endophyte to pathogen are not well understood, particularly in relation to the surrounding fungal community. The objective of the current study was to determine the association of D. sapinea and the composition of the general endophyte community with symptomatic and asymptomatic sites, trees, twigs and tissues in an ongoing outbreak of Diplodia tip blight. The endophytic fungal community was characterized using metabarcoding of the ribosomal ITS2 region. We found that variation in fungal community composition was most influenced by differences between sites, highlighting the importance of site-specific environmental conditions such as previous drought impact and associated crown dieback. However, the fungal communities also varied between symptomatic and asymptomatic trees indicating the significance of tree health. The fungal communities of symptomatic trees, especially in twigs with tip blight symptoms, included D. sapinea, Therrya pini, and Lophodermium arboricola. These results are consistent with the balanced antagonism hypothesis, suggesting that shifts in community composition under stress may facilitate the transition of D. sapinea from a latent endophyte to a pathogen. D. sapinea was found in both healthy and symptomatic twigs, with a ∼60-fold increase in symptomatic tissues. Site differences accounted for 42.6 % of fungal community variation. In contrast, taxa from the order Phaothecales were more abundant in asymptomatic twigs and in healthy tissues of of symptomatic twigs - suggesting potential antagonism. Our findings provide insights into early disease detection and underscore the importance of monitoring endophyte community shifts to support Scots pine forest resilience under climate stress.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.foreco.2025.122781
https://hdl.handle.net/10459.1/468190
url https://doi.org/10.1016/j.foreco.2025.122781
https://hdl.handle.net/10459.1/468190
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI//PID2021-127328OB-I00
Reproducció del document publicat a https://doi.org/10.1016/j.foreco.2025.122781
Forest Ecology and Management, 2025, vol. 589, núm. 122781, p. 1-12
dc.rights.none.fl_str_mv cc-by (c) Brodde et al., 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Brodde et al., 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414330157498368
score 15,811543

Similares en LA Referencia