T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1

Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma p...

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Authors: Maiques, Oscar, Barceló, Carla, Panosa, Anaïs, Pijuan, Jordi, Orgaz, Jose L., Rodríguez Hernández, Irene, Matas Nadal, Clara, Tell Martí, Gemma, Vilella, Ramón, Fabra Fres, Àngels, Puig i Sardà, Susana, Sanz Moreno, Victoria, Matias-Guiu, Xavier, 1958-, Canti, Carles, Herreros, Judit, Marti, Rosa M., Macià, Anna
Format: article
Status:Published version
Publication Date:2018
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/172474
Online Access:https://hdl.handle.net/2445/172474
Access Level:Open access
Keyword:Melanoma
Quimioteràpia
Chemotherapy
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spelling T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1Maiques, OscarBarceló, CarlaPanosa, AnaïsPijuan, JordiOrgaz, Jose L.Rodríguez Hernández, IreneMatas Nadal, ClaraTell Martí, GemmaVilella, RamónFabra Fres, ÀngelsPuig i Sardà, SusanaSanz Moreno, VictoriaMatias-Guiu, Xavier, 1958-Canti, CarlesHerreros, JuditMarti, Rosa M.Macià, AnnaMelanomaQuimioteràpiaMelanomaChemotherapyMelanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3.1 and LC3-II proteins are highly expressed in BRAFV600E compared with NRAS mutant melanomas, both in cell lines and biopsies. Chloroquine, pharmacological blockade, or gene silencing of TTCCs inhibit the autophagic flux and impair the migration and invasion capabilities, specifically in BRAFV600E melanoma cells. Snail1 plays an important role in motility and invasion of melanoma cells. We show that Snail1 is strongly expressed in BRAFV600E melanoma cells and patient biopsies, and its expression decreases when autophagy is blocked. These results demonstrate a role of Snail1 during BRAFV600E melanoma progression and strongly suggest that targeting macroautophagy and, particularly TTCCs, might be a good therapeutic strategy to inhibit metastasis of the most common melanoma type (BRAFV600E).Wiley2020202020182020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/172474Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/pcmr.12690Pigment Cell & Melanoma Research, 2018, vol. 31, num. 4, p. 484-495https://doi.org/10.1111/pcmr.12690(c) John Wiley & Sons, 2018info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1724742026-05-29T05:05:01Z
dc.title.none.fl_str_mv T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
title T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
spellingShingle T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
Maiques, Oscar
Melanoma
Quimioteràpia
Melanoma
Chemotherapy
title_short T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
title_full T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
title_fullStr T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
title_full_unstemmed T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
title_sort T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1
dc.creator.none.fl_str_mv Maiques, Oscar
Barceló, Carla
Panosa, Anaïs
Pijuan, Jordi
Orgaz, Jose L.
Rodríguez Hernández, Irene
Matas Nadal, Clara
Tell Martí, Gemma
Vilella, Ramón
Fabra Fres, Àngels
Puig i Sardà, Susana
Sanz Moreno, Victoria
Matias-Guiu, Xavier, 1958-
Canti, Carles
Herreros, Judit
Marti, Rosa M.
Macià, Anna
author Maiques, Oscar
author_facet Maiques, Oscar
Barceló, Carla
Panosa, Anaïs
Pijuan, Jordi
Orgaz, Jose L.
Rodríguez Hernández, Irene
Matas Nadal, Clara
Tell Martí, Gemma
Vilella, Ramón
Fabra Fres, Àngels
Puig i Sardà, Susana
Sanz Moreno, Victoria
Matias-Guiu, Xavier, 1958-
Canti, Carles
Herreros, Judit
Marti, Rosa M.
Macià, Anna
author_role author
author2 Barceló, Carla
Panosa, Anaïs
Pijuan, Jordi
Orgaz, Jose L.
Rodríguez Hernández, Irene
Matas Nadal, Clara
Tell Martí, Gemma
Vilella, Ramón
Fabra Fres, Àngels
Puig i Sardà, Susana
Sanz Moreno, Victoria
Matias-Guiu, Xavier, 1958-
Canti, Carles
Herreros, Judit
Marti, Rosa M.
Macià, Anna
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Melanoma
Quimioteràpia
Melanoma
Chemotherapy
topic Melanoma
Quimioteràpia
Melanoma
Chemotherapy
description Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3.1 and LC3-II proteins are highly expressed in BRAFV600E compared with NRAS mutant melanomas, both in cell lines and biopsies. Chloroquine, pharmacological blockade, or gene silencing of TTCCs inhibit the autophagic flux and impair the migration and invasion capabilities, specifically in BRAFV600E melanoma cells. Snail1 plays an important role in motility and invasion of melanoma cells. We show that Snail1 is strongly expressed in BRAFV600E melanoma cells and patient biopsies, and its expression decreases when autophagy is blocked. These results demonstrate a role of Snail1 during BRAFV600E melanoma progression and strongly suggest that targeting macroautophagy and, particularly TTCCs, might be a good therapeutic strategy to inhibit metastasis of the most common melanoma type (BRAFV600E).
publishDate 2018
dc.date.none.fl_str_mv 2018
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/172474
url https://hdl.handle.net/2445/172474
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/pcmr.12690
Pigment Cell & Melanoma Research, 2018, vol. 31, num. 4, p. 484-495
https://doi.org/10.1111/pcmr.12690
dc.rights.none.fl_str_mv (c) John Wiley & Sons, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) John Wiley & Sons, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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