Beta-glucan enhances the response to SVCV infection in zebrafish

8 pages, 6 figures

Detalles Bibliográficos
Autores: Medina-Gali, Regla María, Ortega-Villaizán, M. M., Mercado, Luis, Novoa, Beatriz, Coll Morales, Julio, Pérez, Luis
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193246
Acceso en línea:http://hdl.handle.net/10261/193246
Access Level:acceso abierto
Palabra clave:Beta-glucans
Zebrafish
SVCV
Immunostimulant
Antiviral
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spelling Beta-glucan enhances the response to SVCV infection in zebrafishMedina-Gali, Regla MaríaOrtega-Villaizán, M. M.Mercado, LuisNovoa, BeatrizColl Morales, JulioPérez, LuisBeta-glucansZebrafishSVCVImmunostimulantAntiviral8 pages, 6 figuresThe antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression. In vivo, the SVCV-infected fish primed with β-glucan had a higher survival rate (≈73%) than the control SVCV-infected group (≈33%). Additionally, up-regulation of the expression of a set of genes involved in innate immune response was detected in zebrafish intraperitoneally injected of β-glucan: il1b, il6, il8, il10 and tnfa transcripts showed increased expression that appear to be rapid (2 days) but not long-lived (less than 2 weeks). The present study is, to our knowledge, the first to combine cell culture and in vivo approaches to describe host response to β-glucan stimulation and viral infection in zebrafishThis research was supported by Program “I+D+I Orientada a los Retos de la Sociedad” funded by Ministerio de Economía y Competitividad of Spain (Grant AGL2014-51773-C3). Dr. M.M. Ortega-Villaizan contract was supported by the European Research Council (ERC starting grant 2014 GA639249)Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/193246reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-51773-C3-2-Rhttps://doi.org/10.1016/j.dci.2018.02.019Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1932462026-05-22T06:33:51Z
dc.title.none.fl_str_mv Beta-glucan enhances the response to SVCV infection in zebrafish
title Beta-glucan enhances the response to SVCV infection in zebrafish
spellingShingle Beta-glucan enhances the response to SVCV infection in zebrafish
Medina-Gali, Regla María
Beta-glucans
Zebrafish
SVCV
Immunostimulant
Antiviral
title_short Beta-glucan enhances the response to SVCV infection in zebrafish
title_full Beta-glucan enhances the response to SVCV infection in zebrafish
title_fullStr Beta-glucan enhances the response to SVCV infection in zebrafish
title_full_unstemmed Beta-glucan enhances the response to SVCV infection in zebrafish
title_sort Beta-glucan enhances the response to SVCV infection in zebrafish
dc.creator.none.fl_str_mv Medina-Gali, Regla María
Ortega-Villaizán, M. M.
Mercado, Luis
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author Medina-Gali, Regla María
author_facet Medina-Gali, Regla María
Ortega-Villaizán, M. M.
Mercado, Luis
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author_role author
author2 Ortega-Villaizán, M. M.
Mercado, Luis
Novoa, Beatriz
Coll Morales, Julio
Pérez, Luis
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Beta-glucans
Zebrafish
SVCV
Immunostimulant
Antiviral
topic Beta-glucans
Zebrafish
SVCV
Immunostimulant
Antiviral
description 8 pages, 6 figures
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193246
url http://hdl.handle.net/10261/193246
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-51773-C3-2-R
https://doi.org/10.1016/j.dci.2018.02.019
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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