Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids

© 2019 Garcillán-Barcia, Cuartas-Lanza, Cuevas and de la Cruz.

Detalles Bibliográficos
Autores: Garcillán-Barcia, M. Pilar, Cuartas-Lanza, Raquel, Cuevas, Ana, Cruz, Fernando de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/204323
Acceso en línea:http://hdl.handle.net/10261/204323
Access Level:acceso abierto
Palabra clave:Mobilizable plasmids
Horizontal gene transfer
MOBQ relaxase
Cis-acting relaxase
Plasmid coexistence
Bacterial conjugation
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spelling Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 PlasmidsGarcillán-Barcia, M. PilarCuartas-Lanza, RaquelCuevas, AnaCruz, Fernando de laMobilizable plasmidsHorizontal gene transferMOBQ relaxaseCis-acting relaxasePlasmid coexistenceBacterial conjugation© 2019 Garcillán-Barcia, Cuartas-Lanza, Cuevas and de la Cruz.Plasmids are key vehicles of horizontal gene transfer and contribute greatly to bacterial genome plasticity. In this work, we studied a group of plasmids from enterobacteria that encode phylogenetically related mobilization functions that populate the previously non-described MOBQ4 relaxase family. These plasmids encode two transfer genes: mobA coding for the MOBQ4 relaxase; and mobC, which is non-essential but enhances the plasmid mobilization frequency. The origin of transfer is located between these two divergently transcribed mob genes. We found that MPFI conjugative plasmids were the most efficient helpers for MOBQ4 conjugative dissemination among clinically relevant enterobacteria. While highly similar in their mobilization module, two sub-groups with unrelated replicons (Rep_3 and ColE2) can be distinguished in this plasmid family. These subgroups can stably coexist (are compatible) and transfer independently, despite origin-of-transfer cross-recognition by their relaxases. Specific discrimination among their highly similar oriT sequences is guaranteed by the preferential cis activity of the MOBQ4 relaxases. Such a strategy would be biologically relevant in a scenario of co-residence of non-divergent elements to favor self-dissemination.This work was supported by the Spanish Ministry of Economy and Competitiveness (BFU2017-86378-P, AEI/FEDER, UE, to FC) and Consejo Superior de Investigaciones Científicas (201820I143 to MG-B). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Frontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas (España)CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/204323reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-86378-PBFU2017-86378-P/AEI/10.13039/501100011033http://dx.doi.org/10.3389/fmicb.2019.02557Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2043232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
title Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
spellingShingle Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
Garcillán-Barcia, M. Pilar
Mobilizable plasmids
Horizontal gene transfer
MOBQ relaxase
Cis-acting relaxase
Plasmid coexistence
Bacterial conjugation
title_short Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
title_full Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
title_fullStr Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
title_full_unstemmed Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
title_sort Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids
dc.creator.none.fl_str_mv Garcillán-Barcia, M. Pilar
Cuartas-Lanza, Raquel
Cuevas, Ana
Cruz, Fernando de la
author Garcillán-Barcia, M. Pilar
author_facet Garcillán-Barcia, M. Pilar
Cuartas-Lanza, Raquel
Cuevas, Ana
Cruz, Fernando de la
author_role author
author2 Cuartas-Lanza, Raquel
Cuevas, Ana
Cruz, Fernando de la
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mobilizable plasmids
Horizontal gene transfer
MOBQ relaxase
Cis-acting relaxase
Plasmid coexistence
Bacterial conjugation
topic Mobilizable plasmids
Horizontal gene transfer
MOBQ relaxase
Cis-acting relaxase
Plasmid coexistence
Bacterial conjugation
description © 2019 Garcillán-Barcia, Cuartas-Lanza, Cuevas and de la Cruz.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/204323
url http://hdl.handle.net/10261/204323
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-86378-P
BFU2017-86378-P/AEI/10.13039/501100011033
http://dx.doi.org/10.3389/fmicb.2019.02557
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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