Determination of α-tocopherol in pork with high intramuscular fat content
Extraction of α-tocopherol from pork samples with low (3%) (LF) or high (9%) (HF) amount of intramuscular fat have been carried out by three different methods, two of them based in saponification plus extraction of α-tocopherol and the other one without saponification. All samples were spiked with f...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1996 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/102540 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/102540 |
| Access Level: | acceso abierto |
| Palabra clave: | 636.09 Animal tissue Comparison of methods Fat a-tocopherol (determination) Producción animal 3104 Producción Animal 2301 Química Analítica |
| Sumario: | Extraction of α-tocopherol from pork samples with low (3%) (LF) or high (9%) (HF) amount of intramuscular fat have been carried out by three different methods, two of them based in saponification plus extraction of α-tocopherol and the other one without saponification. All samples were spiked with five different amount of α-tocopherol prior to analysis. In LF samples, recovery was in the range 85-95% in all cases, with not significant differences between methods. Recovery was much lower in HF samples when using methods which involve prior saponification of muscle samples (50-60%). Changes in KOH concentration did not improve markedly the recovery. The method based on direct extraction provided much better recovery in HF samples (85-92%) and consequently is recommended for samples high in fat. |
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