Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner
All data relating to this manuscript can be found within the manuscript and its supplementary files. Data that support the findings of this study are available from the corresponding authors upon reasonable request. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Con...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/409846 |
| Acceso en línea: | http://hdl.handle.net/10261/409846 |
| Access Level: | acceso abierto |
| Palabra clave: | AGO1 TST AP-MS miRNA Arabidopsis thaliana FeSOD1 Copper |
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Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| spellingShingle |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner Tomassi, Ariel H. AGO1 TST AP-MS miRNA Arabidopsis thaliana FeSOD1 Copper |
| title_short |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_full |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_fullStr |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_full_unstemmed |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_sort |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| dc.creator.none.fl_str_mv |
Tomassi, Ariel H. Perea García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Daròs Arnau, José Antonio Peñarrubia, Lola Carbonell, Alberto |
| author |
Tomassi, Ariel H. |
| author_facet |
Tomassi, Ariel H. Perea García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Daròs Arnau, José Antonio Peñarrubia, Lola Carbonell, Alberto |
| author_role |
author |
| author2 |
Perea García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Daròs Arnau, José Antonio Peñarrubia, Lola Carbonell, Alberto |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) European Commission Generalitat Valenciana Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
AGO1 TST AP-MS miRNA Arabidopsis thaliana FeSOD1 Copper |
| topic |
AGO1 TST AP-MS miRNA Arabidopsis thaliana FeSOD1 Copper |
| description |
All data relating to this manuscript can be found within the manuscript and its supplementary files. Data that support the findings of this study are available from the corresponding authors upon reasonable request. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al., 2022) partner repository with the dataset identifier PXD057108. |
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2025 |
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2025 2025 2025 |
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info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/409846 |
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http://hdl.handle.net/10261/409846 |
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Inglés |
| language_invalid_str_mv |
Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095118-A-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122186OB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC-2017-21648 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127671NB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116940RB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114691RB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2019-088439 Tomassi, Ariel H.; Perea García, Ana; Rodrigo, Guillermo; Sánchez-Vicente, Javier; Cisneros, Adriana E.; Olmos, Marta; Andrés-Bordería, Amparo; López-Dolz, Lucio; Daròs Arnau, José Antonio; Peñarrubia, Lola; Carbonell, Alberto; 2025; Supplementary data for Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner [Dataset]; Oxford University Press; https://doi.org/10.1093/jxb/eraf270 https://doi.org/10.1093/jxb/eraf270 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Oxford University Press |
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Oxford University Press |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869414288450387968 |
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Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent mannerTomassi, Ariel H.Perea García, AnaRodrigo, GuillermoSánchez-Vicente, JavierCisneros, Adriana E.Olmos, MartaAndrés-Bordería, AmparoLópez-Dolz, LucioDaròs Arnau, José AntonioPeñarrubia, LolaCarbonell, AlbertoAGO1TSTAP-MSmiRNAArabidopsis thalianaFeSOD1CopperAll data relating to this manuscript can be found within the manuscript and its supplementary files. Data that support the findings of this study are available from the corresponding authors upon reasonable request. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al., 2022) partner repository with the dataset identifier PXD057108.Copper (Cu) deficiency compromises plant growth and limits crop productivity. Plants respond to Cu scarcity by activating the expression of several miRNAs, known as Cu-miRNAs, which degrade mRNAs from various cuproproteins to conserve Cu. Cu-miRNAs, like most plant miRNAs, associate with ARGONAUTE 1 (AGO1), the primary effector protein of miRNA-mediated gene silencing pathways, whose function is typically modulated by interacting proteins acting as cofactors. However, how AGO1 is regulated and functions under Cu deficiency remains unknown. Here, we searched for AGO1 interactors in Arabidopsis thaliana plants expressing a functional AGO1 protein tagged with the Twin-Strep-tag (TST) polypeptide, grown under Cu-sufficient or Cu-deficient conditions. TST-AGO1 complexes were affinity purified, and proteins were identified using tandem MS. Interestingly, the iron superoxide dismutase 1 (FeSOD1) encoded by FSD1 was enriched in TST-AGO1 complexes purified from plants grown under Cu deficiency. Moreover, fsd1-2 mutant plants showed reduced levels of AGO1 compared with wild-type plants under Cu sufficiency, while both Cu-miRNA-specific and general AGO1 target mRNAs accumulated to higher levels in fsd1-2 plants under both Cu-deficient and Cu-sufficient conditions compared with wild-type plants. These findings suggest that FeSOD1 is essential for proper AGO1 function, and that its superoxide dismutase activity, which mitigates oxidative stress, enhances AGO1 stability, particularly under Cu deficiency.This work was supported by grants or fellowships from MCIN/AEI/10.13039/501100011033 (co-financed by the European Regional Development Fund) [RTI2018-095118-A-I00, PID2021-122186OB-I00 and RYC-2017-21648 to AC; PID2021-127671NB-I00 to GR; PID2020-116940RB-I00 to LP and APG; BIO2017-83184-R and PID2020-114691RB-I00 to JAD; PRE2019-088439 to AEC], and from the Generalitat Valenciana (CIPROM/2022/21 to GR and JAD).Peer reviewedOxford University PressAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionGeneralitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/409846reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095118-A-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122186OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC-2017-21648info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127671NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116940RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114691RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2019-088439Tomassi, Ariel H.; Perea García, Ana; Rodrigo, Guillermo; Sánchez-Vicente, Javier; Cisneros, Adriana E.; Olmos, Marta; Andrés-Bordería, Amparo; López-Dolz, Lucio; Daròs Arnau, José Antonio; Peñarrubia, Lola; Carbonell, Alberto; 2025; Supplementary data for Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner [Dataset]; Oxford University Press; https://doi.org/10.1093/jxb/eraf270https://doi.org/10.1093/jxb/eraf270Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4098462026-05-22T06:33:51Z |
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15,81155 |