Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex

Most data are available in the main text or the Supplementary Materials. The datasets used and analyzed during the current study are published in the EBRAINS Knowledge Graph (DOI: 10.25493/5F04-N97): Domínguez-Álvaro M, Montero M, Alonso-Nanclares L, Blazquez-Llorca L, Rodríguez J-R, DeFelipe J. (20...

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Autores: Cano-Astorga, Nicolás, DeFelipe, Javier, Alonso-Nanclares, Lidia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/248785
Acceso en línea:http://hdl.handle.net/10261/248785
Access Level:acceso abierto
Palabra clave:Cerebral cortex
Electron microscopy
FIB-SEM
Autopsy
Biopsy
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spelling Three-Dimensional Synaptic Organization of Layer III of the Human Temporal NeocortexCano-Astorga, NicolásDeFelipe, JavierAlonso-Nanclares, LidiaCerebral cortexElectron microscopyFIB-SEMAutopsyBiopsyMost data are available in the main text or the Supplementary Materials. The datasets used and analyzed during the current study are published in the EBRAINS Knowledge Graph (DOI: 10.25493/5F04-N97): Domínguez-Álvaro M, Montero M, Alonso-Nanclares L, Blazquez-Llorca L, Rodríguez J-R, DeFelipe J. (2020). Densities and 3D distributions of synapses using FIB/SEM imaging in the human neocortex (Temporal cortex, T2) [Data set]. Human Brain Project Neuroinformatics Platform.In the present study, we have used Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) to perform a study of the synaptic organization of layer III of Brodmann’s area 21 in human tissue samples obtained from autopsies and biopsies. We analyzed the synaptic density, 3D spatial distribution, and type (asymmetric/symmetric), as well as the size and shape of each synaptic junction of 4945 synapses that were fully reconstructed in 3D. Significant differences in the mean synaptic density between autopsy and biopsy samples were found (0.49 and 0.66 synapses/μm3 , respectively). However, in both types of samples (autopsy and biopsy), the asymmetric:symmetric ratio was similar (93:7) and most asymmetric synapses were established on dendritic spines (75%), while most symmetric synapses were established on dendritic shafts (85%). We also compared several electron microscopy methods and analysis tools to estimate the synaptic density in the same brain tissue. We have shown that FIB/SEM is much more reliable and robust than the majority of the other commonly used EM techniques. The present work constitutes a detailed description of the synaptic organization of cortical layer III. Further studies on the rest of the cortical layers are necessary to better understand the functional organization of this temporal cortical region.This study was funded by grants from the following entities: Spanish “Ministerio de Ciencia e Innovación” grant PGC2018-094307-B-I00, and the Cajal Blue Brain Project made available under aCC-BY-NC 4.0 International license. (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is bioRxiv preprint doi: https://doi.org/10.1101/2021.01.18.427131; this version posted April 8, 2021. The copyright holder for this preprint 59 (the Spanish partner of the Blue Brain Project initiative from EPFL [Switzerland]); Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED, Spain, CB06/05/0066); and the European Union’s Horizon 2020 Framework Programme for Research and Innovation under Specific Grant Agreement No. 945539 (Human Brain Project SGA3). N C-A was awarded a research fellowship from the Spanish “Ministerio de Ciencia e Innovación” (PRE2019-089228).Peer reviewedOxford University PressMinisterio de Ciencia e Innovación (España)Cajal Blue BrainCentro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España)0000-0003-2649-70970000-0001-5484-06600000-0003-3724-0481Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/248785reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grant/PGC2018-094307-B-I00info:eu-repo/grantAgreement/EC/H2020/945539https://academic.oup.com/cercor/advance-article/doi/10.1093/cercor/bhab120/6276652#247808002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2487852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
title Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
spellingShingle Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
Cano-Astorga, Nicolás
Cerebral cortex
Electron microscopy
FIB-SEM
Autopsy
Biopsy
title_short Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
title_full Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
title_fullStr Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
title_full_unstemmed Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
title_sort Three-Dimensional Synaptic Organization of Layer III of the Human Temporal Neocortex
dc.creator.none.fl_str_mv Cano-Astorga, Nicolás
DeFelipe, Javier
Alonso-Nanclares, Lidia
author Cano-Astorga, Nicolás
author_facet Cano-Astorga, Nicolás
DeFelipe, Javier
Alonso-Nanclares, Lidia
author_role author
author2 DeFelipe, Javier
Alonso-Nanclares, Lidia
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Cajal Blue Brain
Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España)
0000-0003-2649-7097
0000-0001-5484-0660
0000-0003-3724-0481
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cerebral cortex
Electron microscopy
FIB-SEM
Autopsy
Biopsy
topic Cerebral cortex
Electron microscopy
FIB-SEM
Autopsy
Biopsy
description Most data are available in the main text or the Supplementary Materials. The datasets used and analyzed during the current study are published in the EBRAINS Knowledge Graph (DOI: 10.25493/5F04-N97): Domínguez-Álvaro M, Montero M, Alonso-Nanclares L, Blazquez-Llorca L, Rodríguez J-R, DeFelipe J. (2020). Densities and 3D distributions of synapses using FIB/SEM imaging in the human neocortex (Temporal cortex, T2) [Data set]. Human Brain Project Neuroinformatics Platform.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/248785
url http://hdl.handle.net/10261/248785
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grant/PGC2018-094307-B-I00
info:eu-repo/grantAgreement/EC/H2020/945539
https://academic.oup.com/cercor/advance-article/doi/10.1093/cercor/bhab120/6276652#247808002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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