Proteolysis of SNAP-25 by types E and A botulinal neurotoxins

Clostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that the light chains of clostridial neurotoxins act as zinc-depend...

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Autores: Binz, Thomas, Blasi Cabús, Joan, Yamasaki, Shinji, Baumeister, Anja, Link, Egenhard, Südhof, Thomas C., Jahn, Reinhard, Niemann, Heiner
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1994
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177645
Acceso en línea:https://hdl.handle.net/2445/177645
Access Level:acceso abierto
Palabra clave:Toxina botulínica
Proteïnes de membrana
Teixit nerviós
Botulinum toxin
Membrane proteins
Nerve tissue
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spelling Proteolysis of SNAP-25 by types E and A botulinal neurotoxinsBinz, ThomasBlasi Cabús, JoanYamasaki, ShinjiBaumeister, AnjaLink, EgenhardSüdhof, Thomas C.Jahn, ReinhardNiemann, HeinerToxina botulínicaProteïnes de membranaTeixit nerviósBotulinum toxinMembrane proteinsNerve tissueClostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that the light chains of clostridial neurotoxins act as zinc-dependent metalloproteases which specifically cleave synaptic target proteins such as synaptobrevin/VAMPs, HPC-1/syntaxin (BoNT/C1), and SNAP-25 (BoNT/A). We show here that BoNT/E, like BoNT/A, cleaves SNAP-25, as generated by in vitro translation or by expression in Escherichia coli. BoNT/E cleaves the Arg180-Ile181 bond. This site is different from that of BoNT/A, which cleaves SNAP-25 between the amino acid residues Gln197 and Arg198. These findings further support the view that clostridial neurotoxins have evolved from an ancestral protease recognizing the exocytotic fusion machinery of synaptic vesicles whereby individual toxins target different members of the membrane fusion complex.American Society for Biochemistry and Molecular Biology1994info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177645Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://www.jbc.org/issue/S0021-9258(17)X4038-6Journal of Biological Chemistry, 1994, vol. 269, num. 3, p. 1617-1620(c) American Society for Biochemistry and Molecular Biology, 1994info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1776452026-05-27T06:46:51Z
dc.title.none.fl_str_mv Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
title Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
spellingShingle Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
Binz, Thomas
Toxina botulínica
Proteïnes de membrana
Teixit nerviós
Botulinum toxin
Membrane proteins
Nerve tissue
title_short Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
title_full Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
title_fullStr Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
title_full_unstemmed Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
title_sort Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
dc.creator.none.fl_str_mv Binz, Thomas
Blasi Cabús, Joan
Yamasaki, Shinji
Baumeister, Anja
Link, Egenhard
Südhof, Thomas C.
Jahn, Reinhard
Niemann, Heiner
author Binz, Thomas
author_facet Binz, Thomas
Blasi Cabús, Joan
Yamasaki, Shinji
Baumeister, Anja
Link, Egenhard
Südhof, Thomas C.
Jahn, Reinhard
Niemann, Heiner
author_role author
author2 Blasi Cabús, Joan
Yamasaki, Shinji
Baumeister, Anja
Link, Egenhard
Südhof, Thomas C.
Jahn, Reinhard
Niemann, Heiner
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxina botulínica
Proteïnes de membrana
Teixit nerviós
Botulinum toxin
Membrane proteins
Nerve tissue
topic Toxina botulínica
Proteïnes de membrana
Teixit nerviós
Botulinum toxin
Membrane proteins
Nerve tissue
description Clostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that the light chains of clostridial neurotoxins act as zinc-dependent metalloproteases which specifically cleave synaptic target proteins such as synaptobrevin/VAMPs, HPC-1/syntaxin (BoNT/C1), and SNAP-25 (BoNT/A). We show here that BoNT/E, like BoNT/A, cleaves SNAP-25, as generated by in vitro translation or by expression in Escherichia coli. BoNT/E cleaves the Arg180-Ile181 bond. This site is different from that of BoNT/A, which cleaves SNAP-25 between the amino acid residues Gln197 and Arg198. These findings further support the view that clostridial neurotoxins have evolved from an ancestral protease recognizing the exocytotic fusion machinery of synaptic vesicles whereby individual toxins target different members of the membrane fusion complex.
publishDate 1994
dc.date.none.fl_str_mv 1994
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177645
url https://hdl.handle.net/2445/177645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://www.jbc.org/issue/S0021-9258(17)X4038-6
Journal of Biological Chemistry, 1994, vol. 269, num. 3, p. 1617-1620
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 1994
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 1994
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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