Double-stranded RNA-mediated interference with plant virus infection

10 p.-1 tab.-5 fig.

Detalles Bibliográficos
Autores: Tenllado, Francisco, Díaz-Ruíz, José Ramón
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/97226
Acceso en línea:http://hdl.handle.net/10261/97226
Access Level:acceso abierto
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spelling Double-stranded RNA-mediated interference with plant virus infectionTenllado, FranciscoDíaz-Ruíz, José Ramón10 p.-1 tab.-5 fig.Double-stranded RNA (dsRNA) has been shown to play a key role as an inducer of different interference phenomena occurring in both the plant and animal kingdoms. Here, we show that dsRNA derived from viral sequences can interfere with virus infection in a sequence-specific manner by directly delivering dsRNA to leaf cells either by mechanical inoculation or via an Agrobacterium-mediated transient-expression assay. We have successfully interfered with the infection of plants by three viruses belonging to the tobamovirus, potyvirus, and alfamovirus groups, demonstrating the reliability of the approach. We suggest that the effect mediated by dsRNA in plant virus infection resembles the analogous phenomenon of RNA interference observed in animals. The interference observed is sequence specific, is dose dependent, and is triggered by dsRNA but not single-stranded RNA. Our results support the view that a dsRNA intermediate in virus replication acts as efficient initiator of posttranscriptional gene silencing (PTGS) in natural virus infections, triggering the initiation step of PTGS that targets viral RNA for degradation.This work was supported by grants BIO97-0615-C02-01, BIO98-0849, BIO2000-1605-C02-02, and BIO2000-0914 from Comisión Interministerial de Ciencia y Tecnología (CICYT) and by grant 07M/0123/2000 from the Dirección General de Investigación de la Comunidad de Madrid. F.T. is a recipient of a contract from Consejo Superior de Investigaciones CientíficasPeer ReviewedAmerican Society for Microbiology2014201420012014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/97226reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/JVI.75.24.12288-12297.2001info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/972262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Double-stranded RNA-mediated interference with plant virus infection
title Double-stranded RNA-mediated interference with plant virus infection
spellingShingle Double-stranded RNA-mediated interference with plant virus infection
Tenllado, Francisco
title_short Double-stranded RNA-mediated interference with plant virus infection
title_full Double-stranded RNA-mediated interference with plant virus infection
title_fullStr Double-stranded RNA-mediated interference with plant virus infection
title_full_unstemmed Double-stranded RNA-mediated interference with plant virus infection
title_sort Double-stranded RNA-mediated interference with plant virus infection
dc.creator.none.fl_str_mv Tenllado, Francisco
Díaz-Ruíz, José Ramón
author Tenllado, Francisco
author_facet Tenllado, Francisco
Díaz-Ruíz, José Ramón
author_role author
author2 Díaz-Ruíz, José Ramón
author2_role author
description 10 p.-1 tab.-5 fig.
publishDate 2001
dc.date.none.fl_str_mv 2001
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/97226
url http://hdl.handle.net/10261/97226
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/JVI.75.24.12288-12297.2001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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