Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions

Designing an electrochemical biosensor with the required features is a complex process involving multiple factors. For instance, nanocomposite materials used ensure the adaptation of the sensor to specific parameters on demand. But beyond making more versatile sensors, these materials likely offer e...

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Detalles Bibliográficos
Autores: Fernández, Laia L., Bastos-Arrieta, Julio, Appelhans, Dietmar, Zhou, Yang, Moreno, Silvia, Palet, Cristina, Baeza, Mireia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/225071
Acceso en línea:https://hdl.handle.net/2445/225071
Access Level:acceso abierto
Palabra clave:Electroquímica
Biosensors
Enzims
Electrochemistry
Enzymes
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spelling Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactionsFernández, Laia L.Bastos-Arrieta, JulioAppelhans, DietmarZhou, YangMoreno, SilviaPalet, CristinaBaeza, MireiaElectroquímicaBiosensorsEnzimsElectrochemistryBiosensorsEnzymesDesigning an electrochemical biosensor with the required features is a complex process involving multiple factors. For instance, nanocomposite materials used ensure the adaptation of the sensor to specific parameters on demand. But beyond making more versatile sensors, these materials likely offer excellent sensing platforms to mimic biological processes (e.g. cell communication) on the electrode surface. For this, developed methods are needed to integrate non-conductive nanoreactors for molecular communication into a biocompatible matrix on electrode surfaces with the request of spatially separated and controlled enzyme localization. Here, we present a novel reduced graphene oxide hybrid material to immobilize polymeric nanoreactors supported by an alginate network as a matrix on the electrode surface. The possibility of introducing cascade reactions in an electrochemical biosensor has the advantage of broadening the target substrates as well as their selectivity using enzymes in different nanocompartments (=compartmentalization). The polymeric vesicles allow the loading of enzymes or artificial enzymes, offering active center retention and long-term enzyme stability. Firstly, the inclusion of spatially separated polymeric active nanocompartments into the matrix on the electrode surface is used to monitor a simple enzyme reaction. However, the study’s accomplishment lies in its successful ability to monitor an enzyme cascade reaction, one producing and the other consuming hydrogen peroxide, hosting natural and artificial enzymes. This proof-of-concept generates an important contribution to the design of analytical platforms capable of detecting complex environments or even monitoring communication between cell-like structures, extremely useful for fields such as synthetic biology, biomimetics and advanced biosensors.Elsevier B.V.2025202520242025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/225071Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.microc.2024.111071Microchemical Journal, 2024https://doi.org/10.1016/j.microc.2024.111071cc-by-nc-nd (c) Fernández, Laia L., et al., 2024http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2250712026-05-29T05:05:01Z
dc.title.none.fl_str_mv Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
title Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
spellingShingle Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
Fernández, Laia L.
Electroquímica
Biosensors
Enzims
Electrochemistry
Biosensors
Enzymes
title_short Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
title_full Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
title_fullStr Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
title_full_unstemmed Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
title_sort Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
dc.creator.none.fl_str_mv Fernández, Laia L.
Bastos-Arrieta, Julio
Appelhans, Dietmar
Zhou, Yang
Moreno, Silvia
Palet, Cristina
Baeza, Mireia
author Fernández, Laia L.
author_facet Fernández, Laia L.
Bastos-Arrieta, Julio
Appelhans, Dietmar
Zhou, Yang
Moreno, Silvia
Palet, Cristina
Baeza, Mireia
author_role author
author2 Bastos-Arrieta, Julio
Appelhans, Dietmar
Zhou, Yang
Moreno, Silvia
Palet, Cristina
Baeza, Mireia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Electroquímica
Biosensors
Enzims
Electrochemistry
Biosensors
Enzymes
topic Electroquímica
Biosensors
Enzims
Electrochemistry
Biosensors
Enzymes
description Designing an electrochemical biosensor with the required features is a complex process involving multiple factors. For instance, nanocomposite materials used ensure the adaptation of the sensor to specific parameters on demand. But beyond making more versatile sensors, these materials likely offer excellent sensing platforms to mimic biological processes (e.g. cell communication) on the electrode surface. For this, developed methods are needed to integrate non-conductive nanoreactors for molecular communication into a biocompatible matrix on electrode surfaces with the request of spatially separated and controlled enzyme localization. Here, we present a novel reduced graphene oxide hybrid material to immobilize polymeric nanoreactors supported by an alginate network as a matrix on the electrode surface. The possibility of introducing cascade reactions in an electrochemical biosensor has the advantage of broadening the target substrates as well as their selectivity using enzymes in different nanocompartments (=compartmentalization). The polymeric vesicles allow the loading of enzymes or artificial enzymes, offering active center retention and long-term enzyme stability. Firstly, the inclusion of spatially separated polymeric active nanocompartments into the matrix on the electrode surface is used to monitor a simple enzyme reaction. However, the study’s accomplishment lies in its successful ability to monitor an enzyme cascade reaction, one producing and the other consuming hydrogen peroxide, hosting natural and artificial enzymes. This proof-of-concept generates an important contribution to the design of analytical platforms capable of detecting complex environments or even monitoring communication between cell-like structures, extremely useful for fields such as synthetic biology, biomimetics and advanced biosensors.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225071
url https://hdl.handle.net/2445/225071
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.microc.2024.111071
Microchemical Journal, 2024
https://doi.org/10.1016/j.microc.2024.111071
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Fernández, Laia L., et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Fernández, Laia L., et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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