Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications

Premi Extraordinari de Doctorat 2016 (promoció del curs 2013-2014)

Detalhes bibliográficos
Autor: Serra, Tiziano
Tipo de documento: tese
Data de publicação:2014
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositório:UPCommons. Portal del coneixement obert de la UPC
Idioma:catalão
OAI Identifier:oai:upcommons.upc.edu:2117/95344
Acesso em linha:https://hdl.handle.net/2117/95344
https://dx.doi.org/10.5821/dissertation-2117-95344
Access Level:Acceso aberto
Palavra-chave:Impressió 3D
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Teixits -- Bastides
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica
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oai_identifier_str oai:upcommons.upc.edu:2117/95344
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
title Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
spellingShingle Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
Serra, Tiziano
Impressió 3D
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Teixits -- Bastides
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica
title_short Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
title_full Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
title_fullStr Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
title_full_unstemmed Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
title_sort Development of 3D-printed biodegradable composite scaffolds for tissue engineering applications
dc.creator.none.fl_str_mv Serra, Tiziano
author Serra, Tiziano
author_facet Serra, Tiziano
author_role author
dc.contributor.none.fl_str_mv Navarro, Melba
dc.subject.none.fl_str_mv Impressió 3D
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Teixits -- Bastides
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica
topic Impressió 3D
Enginyeria de teixits
Medicina regenerativa
Materials biomèdics
Teixits -- Bastides
Ossos -- Regeneració
Àrees temàtiques de la UPC::Enginyeria biomèdica
description Premi Extraordinari de Doctorat 2016 (promoció del curs 2013-2014)
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-04-29
2014
2014-06-27
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/95344
https://dx.doi.org/10.5821/dissertation-2117-95344
url https://hdl.handle.net/2117/95344
https://dx.doi.org/10.5821/dissertation-2117-95344
dc.language.none.fl_str_mv Catalán
cat
language_invalid_str_mv Catalán
language cat
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de Catalunya
publisher.none.fl_str_mv Universitat Politècnica de Catalunya
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414217575038976
spelling Development of 3D-printed biodegradable composite scaffolds for tissue engineering applicationsSerra, TizianoImpressió 3DEnginyeria de teixitsMedicina regenerativaMaterials biomèdicsTeixits -- BastidesOssos -- RegeneracióÀrees temàtiques de la UPC::Enginyeria biomèdicaPremi Extraordinari de Doctorat 2016 (promoció del curs 2013-2014)The design of smart biodegradable scaffolds plays a crucial role in the regeneration of tissues and restoration of their functionality. Advances in material science and manufacturing and in the understanding on the effects of bio-chemical and bio-physical signals on cell behavior, are leading to a new generation of 3D scaffolds. Recent developments in additive manufacturing, also known as 3D-printing, open new exciting challenges in tissue/organ regeneration by means of the fabrication of complex and geometrically precise 3D structures. This thesis aimed the development and characterization of 3D scaffolds for tissue regeneration. For this, a nozzle-based rapid prototyping system was used to combine polylactic acid and a bioactive CaP glass (coded G5) to fabricate 3-D biodegradable scaffolds. Firstly, optimization of the printing conditions represents a key challenge for achieving high quality 3D-printed structures. Thus, we stress the importance of studying the outcome of the plasticizing effect of PEG on PLA-based blends used for the fabrication of 3D-printed scaffolds. Results indicated that the presence of PEG not only improves PLA processing but also leads to relevant surface, geometrical and structural changes including modulation of the degradation rate of PLA-based 3D printed scaffolds. Secondly, the obtained scaffolds were fully characterized from the physic-chemical point of view. Morphological and structural examinations showed that 3D scaffolds had completely interconnected porosity, uniform distribution of the glass particles, and a controlled and repetitive architecture. In addition, incorporation of G5 particles increased both roughness and hydrophilicity of the scaffolds. Compressive modulus was dependent on the scaffold geometry and the presence of glass. Cell study revealed that G5 glass improved mesenchymal stem cell adhesion after 4 h. Additional biological characterization in terms of the inflammatory response were also carried out. Novel studies have pointed towards a decisive role of inflammation in triggering tissue repair and regeneration, while at the same time it is accepted that an exacerbated inflammatory response may lead to rejection of an implant. Thus, understanding and having the capacity to regulate the inflammatory response elicited by 3D scaffolds aimed for tissue regeneration is crucial. In this context, cytokine secretion and cell morphology of human monocytes/macrophages in contact with biodegradable 3D-printed scaffolds (PLA, PLA/G5 and chitosan ones) with different surface properties, architecture and controlled pore geometry was reported. Results revealed that even though the material itself induced the biggest differences, scaffold geometry also affected on the secretion of cytokines. These findings strengthen the appropriateness of these 3D platforms to study modulation of macrophage responses by specific parameters (chemistry, topography, scaffold architecture). Finally, novel scaffolds composed by two phases (PLA and PLA/G5), for use in guided bone regeneration (GBR) were evaluated. Structural, morphological changes were observed during the in vitro degradation of both PLA and PLA/G5 structures. Although mechanical properties decreased, PLA/G5 scaffolds still showed higher compressive modulus than PLA ones, confirming the reinforcing effect of glass particles after immersion time. In vivo implantation was carried out subcutaneously in mice up to 30 days. Results showed that PLA scaffolds induced mononuclear cell without activating any relevant angiogenic process, while PLA/G5 induced higher presence of multinucleated giant cells and consequently stimulated the vascularization process and further tissue regeneration. The technique/materials combination used in this PhD thesis led to the fabrication of promising fully degradable, mechanically stable, bioactive and biocompatible composite scaffolds with well-defined architectures valuable for TE applications.Award-winningUniversitat Politècnica de CatalunyaNavarro, Melba20142014-04-2920142014-06-27doctoral thesishttp://purl.org/coar/resource_type/c_db06VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/2117/95344https://dx.doi.org/10.5821/dissertation-2117-95344reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Cataláncatopen accesshttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/953442026-05-27T15:37:01Z
score 15,300719

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