Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan

The interaction of multiple stressors in freshwater ecosystems may lead to adverse effects on aquatic communities and their ecological functions. Microplastics (MPs) are a class of contaminants of emerging concern that can exert both direct and indirect ecotoxicological effects. A growing number of...

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Autores: Castaño-Ortiz, Jose Maria, Romero Blanch, Ferran, Cojoc, Lorena Cristina, Barceló i Cullerés, Damià, Balcázar, José Luis, Rodríguez Mozaz, Sara, Santos, Lúcia Helena
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/24058
Acceso en línea:http://hdl.handle.net/10256/24058
Access Level:acceso embargado
Palabra clave:Biotransformació (Metabolisme)
Biotransformation (Metabolism)
Contaminants emergents en l'aigua
Emerging contaminants in water
Microplàstics
Microplastics
Toxicologia ambiental
Environmental toxicology
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spelling Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosanCastaño-Ortiz, Jose MariaRomero Blanch, FerranCojoc, Lorena CristinaBarceló i Cullerés, DamiàBalcázar, José LuisRodríguez Mozaz, SaraSantos, Lúcia HelenaBiotransformació (Metabolisme)Biotransformation (Metabolism)Contaminants emergents en l'aiguaEmerging contaminants in waterMicroplàsticsMicroplasticsToxicologia ambientalEnvironmental toxicologyThe interaction of multiple stressors in freshwater ecosystems may lead to adverse effects on aquatic communities and their ecological functions. Microplastics (MPs) are a class of contaminants of emerging concern that can exert both direct and indirect ecotoxicological effects. A growing number of studies have investigated MPs-attached microbial communities, but the interaction between MPs and substrate-associated biofilm (i.e., on natural river substrates, such as stones and sediments) remains poorly studied. In this work, the combined effects of polyethylene MPs (PE-MPs) with a particle size of 10–45 μm (2 mg/L) and the antimicrobial triclosan (TCS) (20 μg/L) were investigated on river biofilms through a short-term exposure experiment (72 h). To the best of authors’ knowledge, this is the first time that the combined effects of MPs and chemical contaminants in substrate-associated river biofilms were assessed. Different response parameters were evaluated, including (i) exposure assessment and ii) contaminants effects at different levels: bacterial community composition, antibiotic resistance, extracellular polymeric substances (EPS), photosynthetic efficiency (Yeff), and leucine aminopeptidase activity (LAPA). Triclosan was accumulated in river biofilms (1189–1513 ng/g dw) alongside its biotransformation product methyl-triclosan (20−29 ng/g dw). Also, PE-MPs were detected on biofilms (168−292 MP/cm2), but they had no significant influence on the bioaccumulation and biotransformation of TCS. A moderate shift in bacterial community composition was driven by TCS, regardless of PE-MPs co-exposure (e.g., increased relative abundance of Sphingomonadaceae family). Additionally, Yeff and EPS content were significantly disrupted in TCS-exposed biofilms. Therefore, the most remarkable effects on river biofilms were related to the antimicrobial TCS, whereas single PE-MPs exposure did not alter any of the evaluated parameters. These results demonstrate that biofilms might act as environmental sink of MPs. Although no interaction between PE-MPs and TCS was observed, the possible indirect impact of other MPs-adsorbed contaminants on biofilms should be further assessedThis work was supported by the Spanish Inter-Ministerial Science and Technology Commission through the ‘PLAS-MED’ project (CICYT, CTM2017-89701-C3). JM Castaño-Ortiz acknowledges the predoctoral grant from AGAUR (2019 FI_B 00881REF). The authors acknowledge the support of the Economy and Knowledge Department of the Catalan Government through a Consolidated Research Group (ICRA-ENV-2021 SGR 01282)Elsevierinfo2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/24058© Environmental Pollution, 2024, art. núm.123369Articles publicats (ICRA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.123369info:eu-repo/semantics/altIdentifier/issn/0269-7491info:eu-repo/semantics/altIdentifier/eissn/1873-6424Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessoai:recercat.cat:10256/240582026-05-29T05:05:01Z
dc.title.none.fl_str_mv Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
title Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
spellingShingle Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
Castaño-Ortiz, Jose Maria
Biotransformació (Metabolisme)
Biotransformation (Metabolism)
Contaminants emergents en l'aigua
Emerging contaminants in water
Microplàstics
Microplastics
Toxicologia ambiental
Environmental toxicology
title_short Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
title_full Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
title_fullStr Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
title_full_unstemmed Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
title_sort Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan
dc.creator.none.fl_str_mv Castaño-Ortiz, Jose Maria
Romero Blanch, Ferran
Cojoc, Lorena Cristina
Barceló i Cullerés, Damià
Balcázar, José Luis
Rodríguez Mozaz, Sara
Santos, Lúcia Helena
author Castaño-Ortiz, Jose Maria
author_facet Castaño-Ortiz, Jose Maria
Romero Blanch, Ferran
Cojoc, Lorena Cristina
Barceló i Cullerés, Damià
Balcázar, José Luis
Rodríguez Mozaz, Sara
Santos, Lúcia Helena
author_role author
author2 Romero Blanch, Ferran
Cojoc, Lorena Cristina
Barceló i Cullerés, Damià
Balcázar, José Luis
Rodríguez Mozaz, Sara
Santos, Lúcia Helena
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biotransformació (Metabolisme)
Biotransformation (Metabolism)
Contaminants emergents en l'aigua
Emerging contaminants in water
Microplàstics
Microplastics
Toxicologia ambiental
Environmental toxicology
topic Biotransformació (Metabolisme)
Biotransformation (Metabolism)
Contaminants emergents en l'aigua
Emerging contaminants in water
Microplàstics
Microplastics
Toxicologia ambiental
Environmental toxicology
description The interaction of multiple stressors in freshwater ecosystems may lead to adverse effects on aquatic communities and their ecological functions. Microplastics (MPs) are a class of contaminants of emerging concern that can exert both direct and indirect ecotoxicological effects. A growing number of studies have investigated MPs-attached microbial communities, but the interaction between MPs and substrate-associated biofilm (i.e., on natural river substrates, such as stones and sediments) remains poorly studied. In this work, the combined effects of polyethylene MPs (PE-MPs) with a particle size of 10–45 μm (2 mg/L) and the antimicrobial triclosan (TCS) (20 μg/L) were investigated on river biofilms through a short-term exposure experiment (72 h). To the best of authors’ knowledge, this is the first time that the combined effects of MPs and chemical contaminants in substrate-associated river biofilms were assessed. Different response parameters were evaluated, including (i) exposure assessment and ii) contaminants effects at different levels: bacterial community composition, antibiotic resistance, extracellular polymeric substances (EPS), photosynthetic efficiency (Yeff), and leucine aminopeptidase activity (LAPA). Triclosan was accumulated in river biofilms (1189–1513 ng/g dw) alongside its biotransformation product methyl-triclosan (20−29 ng/g dw). Also, PE-MPs were detected on biofilms (168−292 MP/cm2), but they had no significant influence on the bioaccumulation and biotransformation of TCS. A moderate shift in bacterial community composition was driven by TCS, regardless of PE-MPs co-exposure (e.g., increased relative abundance of Sphingomonadaceae family). Additionally, Yeff and EPS content were significantly disrupted in TCS-exposed biofilms. Therefore, the most remarkable effects on river biofilms were related to the antimicrobial TCS, whereas single PE-MPs exposure did not alter any of the evaluated parameters. These results demonstrate that biofilms might act as environmental sink of MPs. Although no interaction between PE-MPs and TCS was observed, the possible indirect impact of other MPs-adsorbed contaminants on biofilms should be further assessed
publishDate 2024
dc.date.none.fl_str_mv 2024
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/24058
url http://hdl.handle.net/10256/24058
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.123369
info:eu-repo/semantics/altIdentifier/issn/0269-7491
info:eu-repo/semantics/altIdentifier/eissn/1873-6424
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Environmental Pollution, 2024, art. núm.123369
Articles publicats (ICRA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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repository.mail.fl_str_mv
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