Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes

For efficient transcription, RNA PolII must overcome the presence of nucleosomes. The p38-related MAPK Hog1 is an important regulator of transcription upon osmostress in yeast and thereby it is involved in initiation and elongation. However, the role of this protein kinase in elongation has remained...

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Detalhes bibliográficos
Autores: Mas, Glòria, Nadal, Eulàlia de, Reinhard Dechant, Rodríguez de la Concepción, María Luisa, Logie, Colin, Jimeno González, Silvia, Chávez de Diego, Sebastián
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/55593
Acesso em linha:http://hdl.handle.net/11441/55593
https://doi.org/10.1038/emboj.2008.299
Access Level:acceso abierto
Palavra-chave:chromatin remodelling
gene expression
osmostress
RSC
SAPK Hog1
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spelling Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genesMas, GlòriaNadal, Eulàlia deReinhard DechantRodríguez de la Concepción, María LuisaLogie, ColinJimeno González, SilviaChávez de Diego, Sebastiánchromatin remodellinggene expressionosmostressRSCSAPK Hog1For efficient transcription, RNA PolII must overcome the presence of nucleosomes. The p38-related MAPK Hog1 is an important regulator of transcription upon osmostress in yeast and thereby it is involved in initiation and elongation. However, the role of this protein kinase in elongation has remained unclear. Here, we show that during stress there is a dramatic change in the nucleosome organization of stress-responsive loci that depends on Hog1 and the RSC chromatin remodelling complex. Upon stress, the MAPK Hog1 physically interacts with RSC to direct its association with the ORF of osmo-responsive genes. In RSC mutants, PolII accumulates on stress promoters but not in coding regions. RSC mutants also display reduced stress gene expression and enhanced sensitivity to osmostress. Cell survival under acute osmostress might thus depend on a burst of transcription that in turn could occur only with efficient nucleosome eviction. Our results suggest that the selective targeting of the RSC complex by Hog1 provides the necessary mechanistic basis for this event.España Ministerio de Educacion y Ciencia BFU2006EMBO PressGenéticaMinisterio de Educación y Ciencia (MEC). España2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/55593https://doi.org/10.1038/emboj.2008.299reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésEmbo Journal, 28 (4), 326-336.BFU2006http://dx.doi.org/10.1038/emboj.2008.299info:eu-repo/semantics/openAccessoai:idus.us.es:11441/555932026-06-17T12:51:07Z
dc.title.none.fl_str_mv Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
title Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
spellingShingle Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
Mas, Glòria
chromatin remodelling
gene expression
osmostress
RSC
SAPK Hog1
title_short Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
title_full Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
title_fullStr Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
title_full_unstemmed Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
title_sort Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes
dc.creator.none.fl_str_mv Mas, Glòria
Nadal, Eulàlia de
Reinhard Dechant
Rodríguez de la Concepción, María Luisa
Logie, Colin
Jimeno González, Silvia
Chávez de Diego, Sebastián
author Mas, Glòria
author_facet Mas, Glòria
Nadal, Eulàlia de
Reinhard Dechant
Rodríguez de la Concepción, María Luisa
Logie, Colin
Jimeno González, Silvia
Chávez de Diego, Sebastián
author_role author
author2 Nadal, Eulàlia de
Reinhard Dechant
Rodríguez de la Concepción, María Luisa
Logie, Colin
Jimeno González, Silvia
Chávez de Diego, Sebastián
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
Ministerio de Educación y Ciencia (MEC). España
dc.subject.none.fl_str_mv chromatin remodelling
gene expression
osmostress
RSC
SAPK Hog1
topic chromatin remodelling
gene expression
osmostress
RSC
SAPK Hog1
description For efficient transcription, RNA PolII must overcome the presence of nucleosomes. The p38-related MAPK Hog1 is an important regulator of transcription upon osmostress in yeast and thereby it is involved in initiation and elongation. However, the role of this protein kinase in elongation has remained unclear. Here, we show that during stress there is a dramatic change in the nucleosome organization of stress-responsive loci that depends on Hog1 and the RSC chromatin remodelling complex. Upon stress, the MAPK Hog1 physically interacts with RSC to direct its association with the ORF of osmo-responsive genes. In RSC mutants, PolII accumulates on stress promoters but not in coding regions. RSC mutants also display reduced stress gene expression and enhanced sensitivity to osmostress. Cell survival under acute osmostress might thus depend on a burst of transcription that in turn could occur only with efficient nucleosome eviction. Our results suggest that the selective targeting of the RSC complex by Hog1 provides the necessary mechanistic basis for this event.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/55593
https://doi.org/10.1038/emboj.2008.299
url http://hdl.handle.net/11441/55593
https://doi.org/10.1038/emboj.2008.299
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Embo Journal, 28 (4), 326-336.
BFU2006
http://dx.doi.org/10.1038/emboj.2008.299
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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