Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry

This study presents for the first time an in-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method for the enantiodetermination of drugs of abuse in urine samples. The enantioseparation of R,S-3,4-methylenedioxypyrovalerone (R,S-MDPV) was achieved with a 10 mM am...

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Authors: Pérez-Alcaraz, Albert, Borrull, Francesc, Aguilar, Carme, Calull, Marta, Benavente Moreno, Fernando J. (Julián)
Format: article
Status:Versión aceptada para publicación
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/173106
Online Access:https://hdl.handle.net/2445/173106
Access Level:Open access
Keyword:Espectrometria de masses
Electroforesi capil·lar
Anàlisi d'orina
Mass spectrometry
Capillary electrophoresis
Urine analysis
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spelling Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometryPérez-Alcaraz, AlbertBorrull, FrancescAguilar, CarmeCalull, MartaBenavente Moreno, Fernando J. (Julián)Espectrometria de massesElectroforesi capil·larAnàlisi d'orinaMass spectrometryCapillary electrophoresisUrine analysisThis study presents for the first time an in-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method for the enantiodetermination of drugs of abuse in urine samples. The enantioseparation of R,S-3,4-methylenedioxypyrovalerone (R,S-MDPV) was achieved with a 10 mM ammonium acetate BGE (pH 7) that contained 0.5% (m/v) of sulphated-α-CD as chiral selector. At these pH conditions, this CD was negatively charged, which prevented its entrance into the mass spectrometer since it migrates in the opposite direction. To improve sensitivity, an in-line SPE-CE-MS method using high pressure for sample introduction (i.e. 20 min at 3 bars) was developed. Furthermore, the conditioning procedure and the first part of the electrophoretic separation were performed by switching off the nebulizer gas and the ionization source voltage to avoid non-volatile contaminant arrival into the mass spectrometer. The developed methodology was validated by analyzing urine samples, which required a very simple liquid-liquid extraction (LLE) sample pretreatment. Linearity ranged from 30 to 250 ng/mL, limit of detection (LOD) was 10 ng/mL, relative standard deviation (RSD) values were below 10.5% in terms of intra-day and inter-day precision and the relative error values were below 9% for peak areas accuracy.Elsevier B.V.2021202220202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/173106Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2020.121994Talanta, 2020, vol. 225, p. 121994https://doi.org/10.1016/j.talanta.2020.121994cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1731062026-05-29T05:05:01Z
dc.title.none.fl_str_mv Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
title Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
spellingShingle Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
Pérez-Alcaraz, Albert
Espectrometria de masses
Electroforesi capil·lar
Anàlisi d'orina
Mass spectrometry
Capillary electrophoresis
Urine analysis
title_short Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
title_full Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
title_fullStr Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
title_full_unstemmed Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
title_sort Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry
dc.creator.none.fl_str_mv Pérez-Alcaraz, Albert
Borrull, Francesc
Aguilar, Carme
Calull, Marta
Benavente Moreno, Fernando J. (Julián)
author Pérez-Alcaraz, Albert
author_facet Pérez-Alcaraz, Albert
Borrull, Francesc
Aguilar, Carme
Calull, Marta
Benavente Moreno, Fernando J. (Julián)
author_role author
author2 Borrull, Francesc
Aguilar, Carme
Calull, Marta
Benavente Moreno, Fernando J. (Julián)
author2_role author
author
author
author
dc.subject.none.fl_str_mv Espectrometria de masses
Electroforesi capil·lar
Anàlisi d'orina
Mass spectrometry
Capillary electrophoresis
Urine analysis
topic Espectrometria de masses
Electroforesi capil·lar
Anàlisi d'orina
Mass spectrometry
Capillary electrophoresis
Urine analysis
description This study presents for the first time an in-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method for the enantiodetermination of drugs of abuse in urine samples. The enantioseparation of R,S-3,4-methylenedioxypyrovalerone (R,S-MDPV) was achieved with a 10 mM ammonium acetate BGE (pH 7) that contained 0.5% (m/v) of sulphated-α-CD as chiral selector. At these pH conditions, this CD was negatively charged, which prevented its entrance into the mass spectrometer since it migrates in the opposite direction. To improve sensitivity, an in-line SPE-CE-MS method using high pressure for sample introduction (i.e. 20 min at 3 bars) was developed. Furthermore, the conditioning procedure and the first part of the electrophoretic separation were performed by switching off the nebulizer gas and the ionization source voltage to avoid non-volatile contaminant arrival into the mass spectrometer. The developed methodology was validated by analyzing urine samples, which required a very simple liquid-liquid extraction (LLE) sample pretreatment. Linearity ranged from 30 to 250 ng/mL, limit of detection (LOD) was 10 ng/mL, relative standard deviation (RSD) values were below 10.5% in terms of intra-day and inter-day precision and the relative error values were below 9% for peak areas accuracy.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173106
url https://hdl.handle.net/2445/173106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2020.121994
Talanta, 2020, vol. 225, p. 121994
https://doi.org/10.1016/j.talanta.2020.121994
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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