PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism

Herrerías González, Fernando; Yeramian Hakim, Andree; Baena-Fustegueras, Juan A; Bueno Díez, Marta; Fleitas Pérez, Catherine; de la Fuente, Maricruz; Serrano Casasola, José Carlos Enrique; Granado-Serrano, Ana Belén; Santamaría Gómez, Maite; Yeramian, Nadine; Zorzano-Martínez, Marta; Mora Giral, Concepció; Lecube Torelló, Albert

PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism

Adipocyte dysfunction is the driver of obesity and correlates with insulin resistance and the onset of type 2 diabetes. Protein kinase N1 (PKN1) is a serine/threonine kinase that has been shown to contribute to Glut4 translocation to the membrane and glucose transport. Here, we evaluated the role of...

Descripción completa

Detalles Bibliográficos
Autores:	Herrerías González, Fernando, Yeramian Hakim, Andree, Baena-Fustegueras, Juan A, Bueno Díez, Marta, Fleitas Pérez, Catherine, de la Fuente, Maricruz, Serrano Casasola, José Carlos Enrique, Granado-Serrano, Ana Belén, Santamaría Gómez, Maite, Yeramian, Nadine, Zorzano-Martínez, Marta, Mora Giral, Concepció, Lecube Torelló, Albert
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2023
País:	España
Institución:	Universitat de Lleida (UdL)
Repositorio:	Repositori Obert UdL
OAI Identifier:	oai:repositori.udl.cat:10459.1/463641
Acceso en línea:	https://doi.org/10.3390/nu15102414 https://hdl.handle.net/10459.1/463641
Access Level:	acceso abierto
Palabra clave:	PKN1 Visceral adipose tissue Insulin resistance Type 2 diabetes Adipocyte Glucose metabolism

id	ES_980ef09a71c7eec3f80ec9d6dfef7acc
oai_identifier_str	oai:repositori.udl.cat:10459.1/463641
network_acronym_str	ES
network_name_str	España
repository_id_str
spelling	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose MetabolismHerrerías González, FernandoYeramian Hakim, AndreeBaena-Fustegueras, Juan ABueno Díez, MartaFleitas Pérez, Catherinede la Fuente, MaricruzSerrano Casasola, José Carlos EnriqueGranado-Serrano, Ana BelénSantamaría Gómez, MaiteYeramian, NadineZorzano-Martínez, MartaMora Giral, ConcepcióLecube Torelló, AlbertPKN1Visceral adipose tissueInsulin resistanceType 2 diabetesAdipocyteGlucose metabolismAdipocyte dysfunction is the driver of obesity and correlates with insulin resistance and the onset of type 2 diabetes. Protein kinase N1 (PKN1) is a serine/threonine kinase that has been shown to contribute to Glut4 translocation to the membrane and glucose transport. Here, we evaluated the role of PKN1 in glucose metabolism under insulin-resistant conditions in primary visceral adipose tissue (VAT) from 31 patients with obesity and in murine 3T3-L1 adipocytes. In addition, in vitro studies in human VAT samples and mouse adipocytes were conducted to investigate the role of PKN1 in the adipogenic maturation process and glucose homeostasis control. We show that insulin-resistant adipocytes present a decrease in PKN1 activation levels compared to nondiabetic control counterparts. We further show that PKN1 controls the adipogenesis process and glucose metabolism. PKN1-silenced adipocytes present a decrease in both differentiation process and glucose uptake, with a concomitant decrease in the expression levels of adipogenic markers, such as PPARγ, FABP4, adiponectin and CEBPα. Altogether, these results point to PKN1 as a regulator of key signaling pathways involved in adipocyte differentiation and as an emerging player of adipocyte insulin responsiveness. These findings may provide new therapeutic approaches for the management of insulin resistance in type 2 diabetes.This research was funded by Plan Estratégico de Investigación e Innovación en Salud from the Generalitat de Catalunya, grant number PERIS SLT002/16/000276 and the Instituto de Salud Carlos III (PI18/00964), Fondos FEDER “Una manera de hacer Europa”. C.M. has received funding from Ministerio de industria, Economía y competitividad (Ref.SAF2017-82567-R)MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/nu15102414https://hdl.handle.net/10459.1/463641reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.3390/nu15102414Nutrients 2023, vol. 15, núm. 10cc-by (c)Authors, 2023Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4636412026-06-24T12:42:17Z
dc.title.none.fl_str_mv	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
title	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
spellingShingle	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism Herrerías González, Fernando PKN1 Visceral adipose tissue Insulin resistance Type 2 diabetes Adipocyte Glucose metabolism
title_short	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
title_full	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
title_fullStr	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
title_full_unstemmed	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
title_sort	PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism
dc.creator.none.fl_str_mv	Herrerías González, Fernando Yeramian Hakim, Andree Baena-Fustegueras, Juan A Bueno Díez, Marta Fleitas Pérez, Catherine de la Fuente, Maricruz Serrano Casasola, José Carlos Enrique Granado-Serrano, Ana Belén Santamaría Gómez, Maite Yeramian, Nadine Zorzano-Martínez, Marta Mora Giral, Concepció Lecube Torelló, Albert
author	Herrerías González, Fernando
author_facet	Herrerías González, Fernando Yeramian Hakim, Andree Baena-Fustegueras, Juan A Bueno Díez, Marta Fleitas Pérez, Catherine de la Fuente, Maricruz Serrano Casasola, José Carlos Enrique Granado-Serrano, Ana Belén Santamaría Gómez, Maite Yeramian, Nadine Zorzano-Martínez, Marta Mora Giral, Concepció Lecube Torelló, Albert
author_role	author
author2	Yeramian Hakim, Andree Baena-Fustegueras, Juan A Bueno Díez, Marta Fleitas Pérez, Catherine de la Fuente, Maricruz Serrano Casasola, José Carlos Enrique Granado-Serrano, Ana Belén Santamaría Gómez, Maite Yeramian, Nadine Zorzano-Martínez, Marta Mora Giral, Concepció Lecube Torelló, Albert
author2_role	author author author author author author author author author author author author
dc.subject.none.fl_str_mv	PKN1 Visceral adipose tissue Insulin resistance Type 2 diabetes Adipocyte Glucose metabolism
topic	PKN1 Visceral adipose tissue Insulin resistance Type 2 diabetes Adipocyte Glucose metabolism
description	Adipocyte dysfunction is the driver of obesity and correlates with insulin resistance and the onset of type 2 diabetes. Protein kinase N1 (PKN1) is a serine/threonine kinase that has been shown to contribute to Glut4 translocation to the membrane and glucose transport. Here, we evaluated the role of PKN1 in glucose metabolism under insulin-resistant conditions in primary visceral adipose tissue (VAT) from 31 patients with obesity and in murine 3T3-L1 adipocytes. In addition, in vitro studies in human VAT samples and mouse adipocytes were conducted to investigate the role of PKN1 in the adipogenic maturation process and glucose homeostasis control. We show that insulin-resistant adipocytes present a decrease in PKN1 activation levels compared to nondiabetic control counterparts. We further show that PKN1 controls the adipogenesis process and glucose metabolism. PKN1-silenced adipocytes present a decrease in both differentiation process and glucose uptake, with a concomitant decrease in the expression levels of adipogenic markers, such as PPARγ, FABP4, adiponectin and CEBPα. Altogether, these results point to PKN1 as a regulator of key signaling pathways involved in adipocyte differentiation and as an emerging player of adipocyte insulin responsiveness. These findings may provide new therapeutic approaches for the management of insulin resistance in type 2 diabetes.
publishDate	2023
dc.date.none.fl_str_mv	2023
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.3390/nu15102414 https://hdl.handle.net/10459.1/463641
url	https://doi.org/10.3390/nu15102414 https://hdl.handle.net/10459.1/463641
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.3390/nu15102414 Nutrients 2023, vol. 15, núm. 10
dc.rights.none.fl_str_mv	cc-by (c)Authors, 2023 Attribution 4.0 International info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv	cc-by (c)Authors, 2023 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)
instname_str	Universitat de Lleida (UdL)
reponame_str	Repositori Obert UdL
collection	Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869414162625462272
score	15,811543

PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism

Similares en LA Referencia