Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system

Introduction: Brown rot is the most important fungal disease affecting stone fruit and it is mainly caused by Monilinia fructicola, M. laxa and M. fructigena. Monilinia spp. are necrotrophic plant pathogens with the ability to induce plant cell death by the secretion of different phytotoxic molecule...

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Authors: López , Anselmo, van Kan, Jan A. L., Beenen, Henriek G., Dolcet-Sanjuan, Ramon, Teixidó i Espasa, Neus, Torres, Rosario, Vilanova, Laura
Format: article
Status:Published version
Publication Date:2024
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/467126
Online Access:https://doi.org/10.3389/fpls.2024.1428613
https://hdl.handle.net/10459.1/467126
Access Level:Open access
Keyword:Monilinia fructicola
Prunus spp.
Transient expression
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spelling Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression systemLópez , Anselmovan Kan, Jan A. L.Beenen, Henriek G.Dolcet-Sanjuan, RamonTeixidó i Espasa, NeusTorres, RosarioVilanova, LauraMonilinia fructicolaPrunus spp.Transient expressionIntroduction: Brown rot is the most important fungal disease affecting stone fruit and it is mainly caused by Monilinia fructicola, M. laxa and M. fructigena. Monilinia spp. are necrotrophic plant pathogens with the ability to induce plant cell death by the secretion of different phytotoxic molecules, including proteins or metabolites that are collectively referred to as necrotrophic effectors (NEs). Methods: We exploited the genomes of M. fructicola, M. laxa and M. fructigena to identify their common group of secreted effector proteins and tested the ability of a selected set of effectors to induce cell death in Nicotiana benthamiana, Solanum lycopersicum and Prunus spp. leaves. Results: Fourteen candidate effector genes of M. fructicola, which displayed high expression during infection, were transiently expressed in plants by agroinfiltration using a modified Tobacco Rattle Virus (TRV)-based expression system. Some, but not all, effectors triggered leaf discoloration or cell death in N. benthamiana and S. lycopersicum, which are non-hosts for Monilinia and in Prunus spp., which are the natural hosts. The effector MFRU_030g00190 induced cell death in almost all Prunus genotypes tested, but not in the Solanaceous plants, while MFRU_014g02060, which is an ortholog to BcNep1, caused necrosis in all plant species tested. Conclusion: This method provides opportunities for screening Prunus germplasm with Monilinia effector proteins, to serve as a tool for identifying genetic loci that confer susceptibility to brown rot disease.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by national project PID2020-115702RB-C22/AEI/ 10.13039/501100011033 from the Spanish Government (MINECO) and by funding received from the CERCA Programme SGR-01477/ Generalitat de Catalunya. LV received funding from the postdoctoral fellowships programme Beatriu de Pinós, funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of the European Union (MSCA grant 801370) and AL is recipient of a IRTA Sponsored Fellowship 2022.Frontiers Media2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fpls.2024.1428613https://hdl.handle.net/10459.1/467126reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115702RB-C22Reproducció del document publicat a https://doi.org/10.3389/fpls.2024.1428613Frontiers in Plant Science, 2024, vol. 15, 1428613info:eu-repo/grantAgreement/EC/H2020/801370cc-by (c) Anselmo López et al., 2024Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/4671262026-05-29T05:05:01Z
dc.title.none.fl_str_mv Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
title Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
spellingShingle Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
López , Anselmo
Monilinia fructicola
Prunus spp.
Transient expression
title_short Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
title_full Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
title_fullStr Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
title_full_unstemmed Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
title_sort Evaluation of cell death-inducing activity of Monilinia spp. effectors in several plants using a modified TRV expression system
dc.creator.none.fl_str_mv López , Anselmo
van Kan, Jan A. L.
Beenen, Henriek G.
Dolcet-Sanjuan, Ramon
Teixidó i Espasa, Neus
Torres, Rosario
Vilanova, Laura
author López , Anselmo
author_facet López , Anselmo
van Kan, Jan A. L.
Beenen, Henriek G.
Dolcet-Sanjuan, Ramon
Teixidó i Espasa, Neus
Torres, Rosario
Vilanova, Laura
author_role author
author2 van Kan, Jan A. L.
Beenen, Henriek G.
Dolcet-Sanjuan, Ramon
Teixidó i Espasa, Neus
Torres, Rosario
Vilanova, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Monilinia fructicola
Prunus spp.
Transient expression
topic Monilinia fructicola
Prunus spp.
Transient expression
description Introduction: Brown rot is the most important fungal disease affecting stone fruit and it is mainly caused by Monilinia fructicola, M. laxa and M. fructigena. Monilinia spp. are necrotrophic plant pathogens with the ability to induce plant cell death by the secretion of different phytotoxic molecules, including proteins or metabolites that are collectively referred to as necrotrophic effectors (NEs). Methods: We exploited the genomes of M. fructicola, M. laxa and M. fructigena to identify their common group of secreted effector proteins and tested the ability of a selected set of effectors to induce cell death in Nicotiana benthamiana, Solanum lycopersicum and Prunus spp. leaves. Results: Fourteen candidate effector genes of M. fructicola, which displayed high expression during infection, were transiently expressed in plants by agroinfiltration using a modified Tobacco Rattle Virus (TRV)-based expression system. Some, but not all, effectors triggered leaf discoloration or cell death in N. benthamiana and S. lycopersicum, which are non-hosts for Monilinia and in Prunus spp., which are the natural hosts. The effector MFRU_030g00190 induced cell death in almost all Prunus genotypes tested, but not in the Solanaceous plants, while MFRU_014g02060, which is an ortholog to BcNep1, caused necrosis in all plant species tested. Conclusion: This method provides opportunities for screening Prunus germplasm with Monilinia effector proteins, to serve as a tool for identifying genetic loci that confer susceptibility to brown rot disease.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fpls.2024.1428613
https://hdl.handle.net/10459.1/467126
url https://doi.org/10.3389/fpls.2024.1428613
https://hdl.handle.net/10459.1/467126
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115702RB-C22
Reproducció del document publicat a https://doi.org/10.3389/fpls.2024.1428613
Frontiers in Plant Science, 2024, vol. 15, 1428613
info:eu-repo/grantAgreement/EC/H2020/801370
dc.rights.none.fl_str_mv cc-by (c) Anselmo López et al., 2024
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Anselmo López et al., 2024
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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