Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head

The purpose was to quantify ocular dopamine in rabbits after stimulation of the optic nerve head with short-wavelength (blue) light to activate melanopsin expressed in the axons of intrinsically photosensitive retinal ganglion cells (ipRGCs). Dopamine levels in tears, aqueous humor, vitreous body, a...

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Detalhes bibliográficos
Autores: Carpena Torres, Carlos, Schilling, Tim, Huete Toral, Fernando, Bahmani, Hamed, Carracedo Rodríguez, Juan Gonzalo
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/92480
Acesso em linha:https://hdl.handle.net/20.500.14352/92480
Access Level:acceso abierto
Palavra-chave:617.7
Blue light
Dopamine
Dopaminergic amacrine cells
Intrinsically photosensitive retinal ganglion cells
Melanopsin
Myopia
Óptica y optometría
2209.15 Optometría
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oai_identifier_str oai:docta.ucm.es:20.500.14352/92480
network_acronym_str ES
network_name_str España
repository_id_str
spelling Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve headCarpena Torres, CarlosSchilling, TimHuete Toral, FernandoBahmani, HamedCarracedo Rodríguez, Juan Gonzalo617.7Blue lightDopamineDopaminergic amacrine cellsIntrinsically photosensitive retinal ganglion cellsMelanopsinMyopiaÓptica y optometría2209.15 OptometríaThe purpose was to quantify ocular dopamine in rabbits after stimulation of the optic nerve head with short-wavelength (blue) light to activate melanopsin expressed in the axons of intrinsically photosensitive retinal ganglion cells (ipRGCs). Dopamine levels in tears, aqueous humor, vitreous body, and retina (including choroid) were quantified after blue light stimulation of the optic nerve head of 15 rabbits with an optical fiber for 1 min, 10 min, or no stimulation (n = 5, each group). The left eye of all rabbits was operated on to introduce the optical fiber and stimulate the optic nerve, while the contralateral eye served as internal control. One minute of blue light stimulation significantly increased dopamine concentration in the vitreous body of the treated eyes compared to the contralateral ones (P = 0.015). Stimulation for 10 min significantly increased dopamine concentration in the vitreous body, as well as the aqueous humor (P < 0.05). Therefore, using an optical fiber approach to stimulate the optic nerve head with blue light significantly increased dopamine concentration in the aqueous humor and the vitreous body. This likely reflects an upregulation of retinal dopamine synthesis that could be attributed to ipRGC activation. However, the data provided in this study fell short of establishing a definitive link between dopamine release and ipRGC activation, mainly due to the absence of evidence supporting the expression of the melanopsin photopigment in the optic nerve.ElsevierUniversidad Complutense de Madrid20232023-07-2620232023-07-26journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/92480reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/924802026-06-02T12:44:21Z
dc.title.none.fl_str_mv Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
title Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
spellingShingle Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
Carpena Torres, Carlos
617.7
Blue light
Dopamine
Dopaminergic amacrine cells
Intrinsically photosensitive retinal ganglion cells
Melanopsin
Myopia
Óptica y optometría
2209.15 Optometría
title_short Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
title_full Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
title_fullStr Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
title_full_unstemmed Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
title_sort Increased ocular dopamine levels in rabbits after blue light stimulation of the optic nerve head
dc.creator.none.fl_str_mv Carpena Torres, Carlos
Schilling, Tim
Huete Toral, Fernando
Bahmani, Hamed
Carracedo Rodríguez, Juan Gonzalo
author Carpena Torres, Carlos
author_facet Carpena Torres, Carlos
Schilling, Tim
Huete Toral, Fernando
Bahmani, Hamed
Carracedo Rodríguez, Juan Gonzalo
author_role author
author2 Schilling, Tim
Huete Toral, Fernando
Bahmani, Hamed
Carracedo Rodríguez, Juan Gonzalo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 617.7
Blue light
Dopamine
Dopaminergic amacrine cells
Intrinsically photosensitive retinal ganglion cells
Melanopsin
Myopia
Óptica y optometría
2209.15 Optometría
topic 617.7
Blue light
Dopamine
Dopaminergic amacrine cells
Intrinsically photosensitive retinal ganglion cells
Melanopsin
Myopia
Óptica y optometría
2209.15 Optometría
description The purpose was to quantify ocular dopamine in rabbits after stimulation of the optic nerve head with short-wavelength (blue) light to activate melanopsin expressed in the axons of intrinsically photosensitive retinal ganglion cells (ipRGCs). Dopamine levels in tears, aqueous humor, vitreous body, and retina (including choroid) were quantified after blue light stimulation of the optic nerve head of 15 rabbits with an optical fiber for 1 min, 10 min, or no stimulation (n = 5, each group). The left eye of all rabbits was operated on to introduce the optical fiber and stimulate the optic nerve, while the contralateral eye served as internal control. One minute of blue light stimulation significantly increased dopamine concentration in the vitreous body of the treated eyes compared to the contralateral ones (P = 0.015). Stimulation for 10 min significantly increased dopamine concentration in the vitreous body, as well as the aqueous humor (P < 0.05). Therefore, using an optical fiber approach to stimulate the optic nerve head with blue light significantly increased dopamine concentration in the aqueous humor and the vitreous body. This likely reflects an upregulation of retinal dopamine synthesis that could be attributed to ipRGC activation. However, the data provided in this study fell short of establishing a definitive link between dopamine release and ipRGC activation, mainly due to the absence of evidence supporting the expression of the melanopsin photopigment in the optic nerve.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-07-26
2023
2023-07-26
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/92480
url https://hdl.handle.net/20.500.14352/92480
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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