Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.

Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regula...

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Autores: Alen, Francisco, Crespo, Inmaculada, Ramírez-López, María Teresa, Jagerovic, Nadine, Goya, Pilar, Rodríguez de Fonseca, Fernando, Gómez de Heras, Raquel, Orio, Laura
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17048
Acceso en línea:http://hdl.handle.net/20.500.12105/17048
Access Level:acceso abierto
Palabra clave:Animales
Ghrelina
Masculino
Piperidinas
Ratas
Ratas Wistar
Ingestión de Alimentos
Receptor cannabinoide CB1
Eating
Ghrelin
Male
Piperidines
Pyrazoles
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1
Triazoles
Animals
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network_acronym_str ES
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repository_id_str
spelling Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.Alen, FranciscoCrespo, InmaculadaRamírez-López, María TeresaJagerovic, NadineGoya, PilarRodríguez de Fonseca, FernandoGómez de Heras, RaquelOrio, LauraAnimalesGhrelinaMasculinoPiperidinasRatasRatas WistarIngestión de AlimentosReceptor cannabinoide CB1EatingGhrelinMalePiperidinesPyrazolesRatsRats, WistarReceptor, Cannabinoid, CB1TriazolesAnimalsGhrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regulate CB1-mediated control of food intake and a functional relationship between hypothalamic ghrelin and cannabinoid CB1 receptor has been proposed. First of all, we investigated brain ghrelin actions on food intake in rats with different metabolic status (negative or equilibrate energy balance). Secondly, we tested a sub-anxiogenic ultra-low dose of the CB1 antagonist SR141716A (Rimonabant) and the peripheral-acting CB1 antagonist LH-21 on ghrelin orexigenic actions. We found that: 1) central administration of ghrelin promotes food intake in free feeding animals but not in 24 h food-deprived or chronically food-restricted animals; 2) an ultra-low dose of SR141716A (a subthreshold dose 75 folds lower than the EC50 for induction of anxiety) completely counteracts the orexigenic actions of central ghrelin in free feeding animals; 3) the peripheral-restricted CB1 antagonist LH-21 blocks ghrelin-induced hyperphagia in free feeding animals. Our study highlights the importance of the animaĺs metabolic status for the effectiveness of ghrelin in promoting feeding, and suggests that the peripheral endocannabinoid system may interact with ghrelińs signal in the control of food intake under equilibrate energy balance conditions.Public Library of Science (PLOS)[Alen,F; Crespo,I Ramírez-López,MT; Rodríguez de Fonseca,F; Gómez de Heras,R; Orio,L] Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, Spain. [Jagerovic,N; Goya,P] Instituto de Química Médica, Centro Superior de Investigaciones Científicas, Madrid, Spain. [Rodríguez de Fonseca,F] Hospital Carlos Haya, Fundación Pública Andaluza para la Investigación en Málaga en Biomedicina y Salud (FIMABIS), Málaga, Spain. Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.20242024-01-1520132013-04-0220132013-04-02research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/17048reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/170482026-06-12T12:43:37Z
dc.title.none.fl_str_mv Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
title Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
spellingShingle Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
Alen, Francisco
Animales
Ghrelina
Masculino
Piperidinas
Ratas
Ratas Wistar
Ingestión de Alimentos
Receptor cannabinoide CB1
Eating
Ghrelin
Male
Piperidines
Pyrazoles
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1
Triazoles
Animals
title_short Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
title_full Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
title_fullStr Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
title_full_unstemmed Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
title_sort Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
dc.creator.none.fl_str_mv Alen, Francisco
Crespo, Inmaculada
Ramírez-López, María Teresa
Jagerovic, Nadine
Goya, Pilar
Rodríguez de Fonseca, Fernando
Gómez de Heras, Raquel
Orio, Laura
author Alen, Francisco
author_facet Alen, Francisco
Crespo, Inmaculada
Ramírez-López, María Teresa
Jagerovic, Nadine
Goya, Pilar
Rodríguez de Fonseca, Fernando
Gómez de Heras, Raquel
Orio, Laura
author_role author
author2 Crespo, Inmaculada
Ramírez-López, María Teresa
Jagerovic, Nadine
Goya, Pilar
Rodríguez de Fonseca, Fernando
Gómez de Heras, Raquel
Orio, Laura
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv [Alen,F; Crespo,I Ramírez-López,MT; Rodríguez de Fonseca,F; Gómez de Heras,R; Orio,L] Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, Spain. [Jagerovic,N; Goya,P] Instituto de Química Médica, Centro Superior de Investigaciones Científicas, Madrid, Spain. [Rodríguez de Fonseca,F] Hospital Carlos Haya, Fundación Pública Andaluza para la Investigación en Málaga en Biomedicina y Salud (FIMABIS), Málaga, Spain. Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.
dc.subject.none.fl_str_mv Animales
Ghrelina
Masculino
Piperidinas
Ratas
Ratas Wistar
Ingestión de Alimentos
Receptor cannabinoide CB1
Eating
Ghrelin
Male
Piperidines
Pyrazoles
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1
Triazoles
Animals
topic Animales
Ghrelina
Masculino
Piperidinas
Ratas
Ratas Wistar
Ingestión de Alimentos
Receptor cannabinoide CB1
Eating
Ghrelin
Male
Piperidines
Pyrazoles
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1
Triazoles
Animals
description Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regulate CB1-mediated control of food intake and a functional relationship between hypothalamic ghrelin and cannabinoid CB1 receptor has been proposed. First of all, we investigated brain ghrelin actions on food intake in rats with different metabolic status (negative or equilibrate energy balance). Secondly, we tested a sub-anxiogenic ultra-low dose of the CB1 antagonist SR141716A (Rimonabant) and the peripheral-acting CB1 antagonist LH-21 on ghrelin orexigenic actions. We found that: 1) central administration of ghrelin promotes food intake in free feeding animals but not in 24 h food-deprived or chronically food-restricted animals; 2) an ultra-low dose of SR141716A (a subthreshold dose 75 folds lower than the EC50 for induction of anxiety) completely counteracts the orexigenic actions of central ghrelin in free feeding animals; 3) the peripheral-restricted CB1 antagonist LH-21 blocks ghrelin-induced hyperphagia in free feeding animals. Our study highlights the importance of the animaĺs metabolic status for the effectiveness of ghrelin in promoting feeding, and suggests that the peripheral endocannabinoid system may interact with ghrelińs signal in the control of food intake under equilibrate energy balance conditions.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-04-02
2013
2013-04-02
2024
2024-01-15
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/17048
url http://hdl.handle.net/20.500.12105/17048
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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